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T cells from BB-DP rats show a unique cytokine mRNA profile associated with the IDDMI susceptibility gene, LYP

Gold, Daniel P. ; Shaikewitz, Samuel T. ; Mueller, Deanna ; Redd, Joan R. ; Sellins, Karen S. ; Pettersson, Anna ; Lernmark, Ake LU orcid and Bellgrau, Donald (1995) In Autoimmunity 22(3). p.149-161
Abstract

Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4+ and CD8+ peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that... (More)

Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4+ and CD8+ peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that previously described for mouse medullary thymocytes. In addition, cell surface staining of peripheral T cells from diabetes prone animals revealed a high frequency of Thyl+ cells, which is characteristic of both thymocytes and recent thymic emigrants. Following thymectomy, T cell responsiveness to a number of different stimuli is greatly reduced on a cell for cell basis as is the absolute number of surviving T cells. Taken collectively, our results suggest that the majority of the peripheral T cell pool in these diabetic prone rats consists of short lived, recent thymic emigrants which most likely also contain the effector cells required for initiation of diabetes.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
BB rats, Lymphopenia, T cell development
in
Autoimmunity
volume
22
issue
3
pages
149 - 161
publisher
Taylor & Francis
external identifiers
  • scopus:0029553280
  • pmid:8734569
ISSN
0891-6934
DOI
10.3109/08916939508995312
language
English
LU publication?
no
id
76deba47-4d2e-4865-a011-9fc088e048f2
date added to LUP
2019-08-08 11:37:26
date last changed
2024-03-13 08:02:57
@article{76deba47-4d2e-4865-a011-9fc088e048f2,
  abstract     = {{<p>Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4<sup>+</sup> and CD8<sup>+</sup> peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that previously described for mouse medullary thymocytes. In addition, cell surface staining of peripheral T cells from diabetes prone animals revealed a high frequency of Thyl<sup>+</sup> cells, which is characteristic of both thymocytes and recent thymic emigrants. Following thymectomy, T cell responsiveness to a number of different stimuli is greatly reduced on a cell for cell basis as is the absolute number of surviving T cells. Taken collectively, our results suggest that the majority of the peripheral T cell pool in these diabetic prone rats consists of short lived, recent thymic emigrants which most likely also contain the effector cells required for initiation of diabetes.</p>}},
  author       = {{Gold, Daniel P. and Shaikewitz, Samuel T. and Mueller, Deanna and Redd, Joan R. and Sellins, Karen S. and Pettersson, Anna and Lernmark, Ake and Bellgrau, Donald}},
  issn         = {{0891-6934}},
  keywords     = {{BB rats; Lymphopenia; T cell development}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{3}},
  pages        = {{149--161}},
  publisher    = {{Taylor & Francis}},
  series       = {{Autoimmunity}},
  title        = {{T cells from BB-DP rats show a unique cytokine mRNA profile associated with the IDDMI susceptibility gene, LYP}},
  url          = {{http://dx.doi.org/10.3109/08916939508995312}},
  doi          = {{10.3109/08916939508995312}},
  volume       = {{22}},
  year         = {{1995}},
}