T cells from BB-DP rats show a unique cytokine mRNA profile associated with the IDDMI susceptibility gene, LYP
(1995) In Autoimmunity 22(3). p.149-161- Abstract
Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4+ and CD8+ peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that... (More)
Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4+ and CD8+ peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that previously described for mouse medullary thymocytes. In addition, cell surface staining of peripheral T cells from diabetes prone animals revealed a high frequency of Thyl+ cells, which is characteristic of both thymocytes and recent thymic emigrants. Following thymectomy, T cell responsiveness to a number of different stimuli is greatly reduced on a cell for cell basis as is the absolute number of surviving T cells. Taken collectively, our results suggest that the majority of the peripheral T cell pool in these diabetic prone rats consists of short lived, recent thymic emigrants which most likely also contain the effector cells required for initiation of diabetes.
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- author
- Gold, Daniel P. ; Shaikewitz, Samuel T. ; Mueller, Deanna ; Redd, Joan R. ; Sellins, Karen S. ; Pettersson, Anna ; Lernmark, Ake LU and Bellgrau, Donald
- publishing date
- 1995-01-01
- type
- Contribution to journal
- publication status
- published
- keywords
- BB rats, Lymphopenia, T cell development
- in
- Autoimmunity
- volume
- 22
- issue
- 3
- pages
- 149 - 161
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0029553280
- pmid:8734569
- ISSN
- 0891-6934
- DOI
- 10.3109/08916939508995312
- language
- English
- LU publication?
- no
- id
- 76deba47-4d2e-4865-a011-9fc088e048f2
- date added to LUP
- 2019-08-08 11:37:26
- date last changed
- 2024-03-13 08:02:57
@article{76deba47-4d2e-4865-a011-9fc088e048f2, abstract = {{<p>Diabetes prone biobreeding rats display several abnormalites in T cell numbers, T cell function and T cell surface phenotype which are associated with the onset of spontaneous disease. One of the most pronounced abnormalities in these animals is a marked T cell lymphopenia which is evident in both CD4<sup>+</sup> and CD8<sup>+</sup> peripheral T cell subsets. To gain a better understanding as to the nature of T cell responses in these animals, we have utilized RT-PCR to analyze the cytokine mRNA profiles of mitogen activated peripheral T cells derived from lymphopenic and non-lymphopenic animals. Our results suggest that inheritance of the lymphopenia gene, Lyp, is associated with a unique cytokine profile most similar to that previously described for mouse medullary thymocytes. In addition, cell surface staining of peripheral T cells from diabetes prone animals revealed a high frequency of Thyl<sup>+</sup> cells, which is characteristic of both thymocytes and recent thymic emigrants. Following thymectomy, T cell responsiveness to a number of different stimuli is greatly reduced on a cell for cell basis as is the absolute number of surviving T cells. Taken collectively, our results suggest that the majority of the peripheral T cell pool in these diabetic prone rats consists of short lived, recent thymic emigrants which most likely also contain the effector cells required for initiation of diabetes.</p>}}, author = {{Gold, Daniel P. and Shaikewitz, Samuel T. and Mueller, Deanna and Redd, Joan R. and Sellins, Karen S. and Pettersson, Anna and Lernmark, Ake and Bellgrau, Donald}}, issn = {{0891-6934}}, keywords = {{BB rats; Lymphopenia; T cell development}}, language = {{eng}}, month = {{01}}, number = {{3}}, pages = {{149--161}}, publisher = {{Taylor & Francis}}, series = {{Autoimmunity}}, title = {{T cells from BB-DP rats show a unique cytokine mRNA profile associated with the IDDMI susceptibility gene, LYP}}, url = {{http://dx.doi.org/10.3109/08916939508995312}}, doi = {{10.3109/08916939508995312}}, volume = {{22}}, year = {{1995}}, }