Bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase
(2003) Seventh World Congress on Biosensors In Biosensors & Bioelectronics 18(5-6). p.705-714- Abstract
- Amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. The H<sub>2</sub>O<sub>2</sub> produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. All developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (SPP) cross-linked to a redox polymer. The developed electrodes were characterized (sensitivity, stability, and performances in organic medium) and compared with similarly built ones using the 'classical' horseradish peroxidase... (More)
- Amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. The H<sub>2</sub>O<sub>2</sub> produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. All developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (SPP) cross-linked to a redox polymer. The developed electrodes were characterized (sensitivity, stability, and performances in organic medium) and compared with similarly built ones using the 'classical' horseradish peroxidase (HRP). The SPP-based electrodes displayed higher sensitivity and better detection limit for putrescine than those using HRP and were also shown to retain their activity in organic phase much better than the HPR based ones. The importance of attractive or repulsive electrostatic interactions between the peroxidases and oxidases (determined by their isoelectric points) were found to play an important role in the sensitivity of the obtained sensors (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/907627
- author
- Castillo Leon, Jaime LU ; Gaspar, Szilveszter LU ; Sakharov, I and Csöregi, Elisabeth LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- amperometric biosensor, bienzyme electrode, redox hydrogel, sweet, potato peroxidase
- in
- Biosensors & Bioelectronics
- volume
- 18
- issue
- 5-6
- pages
- 10 pages
- publisher
- Elsevier
- conference name
- Seventh World Congress on Biosensors
- conference location
- Kyoto, Japan
- conference dates
- 2002-05-15 - 2002-05-17
- external identifiers
-
- wos:000182790500028
- pmid:12706582
- scopus:0037403202
- ISSN
- 1873-4235
- DOI
- 10.1016/S0956-5663(03)00011-3
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 76e40448-c503-474c-a3f5-2723d23bfa46 (old id 907627)
- date added to LUP
- 2016-04-01 16:05:31
- date last changed
- 2022-01-28 17:11:50
@article{76e40448-c503-474c-a3f5-2723d23bfa46,
abstract = {{Amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. The H<sub>2</sub>O<sub>2</sub> produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. All developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (SPP) cross-linked to a redox polymer. The developed electrodes were characterized (sensitivity, stability, and performances in organic medium) and compared with similarly built ones using the 'classical' horseradish peroxidase (HRP). The SPP-based electrodes displayed higher sensitivity and better detection limit for putrescine than those using HRP and were also shown to retain their activity in organic phase much better than the HPR based ones. The importance of attractive or repulsive electrostatic interactions between the peroxidases and oxidases (determined by their isoelectric points) were found to play an important role in the sensitivity of the obtained sensors}},
author = {{Castillo Leon, Jaime and Gaspar, Szilveszter and Sakharov, I and Csöregi, Elisabeth}},
issn = {{1873-4235}},
keywords = {{amperometric biosensor; bienzyme electrode; redox hydrogel; sweet; potato peroxidase}},
language = {{eng}},
number = {{5-6}},
pages = {{705--714}},
publisher = {{Elsevier}},
series = {{Biosensors & Bioelectronics}},
title = {{Bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase}},
url = {{http://dx.doi.org/10.1016/S0956-5663(03)00011-3}},
doi = {{10.1016/S0956-5663(03)00011-3}},
volume = {{18}},
year = {{2003}},
}