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A method for time-resolved calorespirometry of terrestrial samples.

Wadsö, Lars LU (2015) In Methods 76(Online 23 October 2014). p.20-26
Abstract
A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide... (More)
A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (<1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide production - tend to be quite high, improvements to the technique are also discussed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods
volume
76
issue
Online 23 October 2014
pages
20 - 26
publisher
Elsevier
external identifiers
  • pmid:25449900
  • wos:000352332000004
  • scopus:84925397250
  • pmid:25449900
ISSN
1095-9130
DOI
10.1016/j.ymeth.2014.10.001
language
English
LU publication?
yes
id
76fa10a7-6c3e-4b5f-878f-481d2b954cb4 (old id 4913294)
date added to LUP
2016-04-01 10:37:17
date last changed
2022-01-26 00:56:27
@article{76fa10a7-6c3e-4b5f-878f-481d2b954cb4,
  abstract     = {{A new vessel for simultaneous isothermal calorimetry and respirometry (calorespirometry) on terrestrial (non-aqueous) samples has been developed. All types of small (&lt;1g) biological samples (insects, soil, leaves, fungi, etc.) can be studied. The respirometric measurements are made by opening and closing a valve to a vial inside the sample ampoule containing a carbon dioxide absorbent. Typically a 7h measurement results in seven measurements of heat production rate, oxygen consumption and carbon dioxide production, which can be used to evaluate how the metabolic activity in a sample changes over time. Results from three experiments on leaves, a cut vegetable, and mold are given. As uncertainties - especially in the carbon dioxide production - tend to be quite high, improvements to the technique are also discussed.}},
  author       = {{Wadsö, Lars}},
  issn         = {{1095-9130}},
  language     = {{eng}},
  number       = {{Online 23 October 2014}},
  pages        = {{20--26}},
  publisher    = {{Elsevier}},
  series       = {{Methods}},
  title        = {{A method for time-resolved calorespirometry of terrestrial samples.}},
  url          = {{http://dx.doi.org/10.1016/j.ymeth.2014.10.001}},
  doi          = {{10.1016/j.ymeth.2014.10.001}},
  volume       = {{76}},
  year         = {{2015}},
}