Evaluation of quantitative thin layer chromatography using staining reagents
(2007) In Journal of Chromatography A 1164(1-2). p.298-305- Abstract
- Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/656570
- author
- Johnsson, Richard LU ; Traff, Gustav ; Sundén, Martin LU and Ellervik, Ulf LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- quantification, TLC, image analysis software, staining reagents
- in
- Journal of Chromatography A
- volume
- 1164
- issue
- 1-2
- pages
- 298 - 305
- publisher
- Elsevier
- external identifiers
-
- wos:000249471900037
- scopus:34548037947
- ISSN
- 0021-9673
- DOI
- 10.1016/j.chroma.2007.07.029
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)
- id
- 771476c7-1dcb-42de-bac8-b2a7cfe708ef (old id 656570)
- date added to LUP
- 2016-04-01 15:27:24
- date last changed
- 2024-01-10 15:25:09
@article{771476c7-1dcb-42de-bac8-b2a7cfe708ef, abstract = {{Thin layer chromatography (TLC) using staining reagents is a superior method for analyzing organic compounds without chromophores. It is fast, versatile and sometimes the only viable method. We have investigated quantitative TLC using staining reagents, in combination with modem image analysis software. Our results show that it is possible to get reliable measurements, suitable for high-throughput screening or physical organic investigations. The range of detection and the errors for the different parts of the process are illustrated. We show that the errors are largely due to the staining process and can be diminished by measuring ratios of compounds. (c) 2007 Elsevier B.V. All rights reserved.}}, author = {{Johnsson, Richard and Traff, Gustav and Sundén, Martin and Ellervik, Ulf}}, issn = {{0021-9673}}, keywords = {{quantification; TLC; image analysis software; staining reagents}}, language = {{eng}}, number = {{1-2}}, pages = {{298--305}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{Evaluation of quantitative thin layer chromatography using staining reagents}}, url = {{http://dx.doi.org/10.1016/j.chroma.2007.07.029}}, doi = {{10.1016/j.chroma.2007.07.029}}, volume = {{1164}}, year = {{2007}}, }