The molecular mechanism for the genetic disorder familial defective apolipoprotein B100
(2001) In Journal of Biological Chemistry 276(12). p.9214-9218- Abstract
- Familial defective apolipoprotein B100 (FDB) is a genetic disorder in which low density lipoproteins (LDL) bind defectively to the LDL receptor, resulting in hypercholesterolemia and premature atherosclerosis. FDB is caused by a mutation (R3500Q) that changes the conformation of apolipoprotein (apo) B100 near the receptor-binding site. We previously showed that arginine, not simply a positive charge, at residue 3500 is essential for normal receptor binding and that the carboxyl terminus of apoB100 is necessary for mutations affecting arginine 3500 to disrupt LDL receptor binding. Thus, normal receptor binding involves an interaction between arginine 3500 and tryptophan 4369 in the carboxyl tail of apoB100. W4369Y LDL and R3500Q LDL... (More)
- Familial defective apolipoprotein B100 (FDB) is a genetic disorder in which low density lipoproteins (LDL) bind defectively to the LDL receptor, resulting in hypercholesterolemia and premature atherosclerosis. FDB is caused by a mutation (R3500Q) that changes the conformation of apolipoprotein (apo) B100 near the receptor-binding site. We previously showed that arginine, not simply a positive charge, at residue 3500 is essential for normal receptor binding and that the carboxyl terminus of apoB100 is necessary for mutations affecting arginine 3500 to disrupt LDL receptor binding. Thus, normal receptor binding involves an interaction between arginine 3500 and tryptophan 4369 in the carboxyl tail of apoB100. W4369Y LDL and R3500Q LDL isolated from transgenic mice had identically defective LDL binding and a higher affinity for the monoclonal antibody MB47, which has an epitope flanking residue 3500. We conclude that arginine 3500 interacts with tryptophan 4369 and facilitates the conformation of apoB100 required for normal receptor binding of LDL. From our findings, we developed a model that explains how the carboxyl terminus of apoB100 interacts with the backbone of apoB100 that enwraps the LDL particle. Our model also explains how all known ligand-defective mutations in apoB100, including a newly discovered R3480W mutation in apoB100, cause defective receptor binding. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1120817
- author
- Boren, Jan ; Ekström, Ulf LU ; Ågren, Bo ; Nilsson-Ehle, Peter LU and Innerarity, Thomas L
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 276
- issue
- 12
- pages
- 9214 - 9218
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:11115503
- scopus:0035937832
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M008890200
- language
- English
- LU publication?
- yes
- id
- 771a431d-fd36-4ea1-b393-877ed8710a31 (old id 1120817)
- date added to LUP
- 2016-04-01 11:55:01
- date last changed
- 2022-04-13 03:14:01
@article{771a431d-fd36-4ea1-b393-877ed8710a31, abstract = {{Familial defective apolipoprotein B100 (FDB) is a genetic disorder in which low density lipoproteins (LDL) bind defectively to the LDL receptor, resulting in hypercholesterolemia and premature atherosclerosis. FDB is caused by a mutation (R3500Q) that changes the conformation of apolipoprotein (apo) B100 near the receptor-binding site. We previously showed that arginine, not simply a positive charge, at residue 3500 is essential for normal receptor binding and that the carboxyl terminus of apoB100 is necessary for mutations affecting arginine 3500 to disrupt LDL receptor binding. Thus, normal receptor binding involves an interaction between arginine 3500 and tryptophan 4369 in the carboxyl tail of apoB100. W4369Y LDL and R3500Q LDL isolated from transgenic mice had identically defective LDL binding and a higher affinity for the monoclonal antibody MB47, which has an epitope flanking residue 3500. We conclude that arginine 3500 interacts with tryptophan 4369 and facilitates the conformation of apoB100 required for normal receptor binding of LDL. From our findings, we developed a model that explains how the carboxyl terminus of apoB100 interacts with the backbone of apoB100 that enwraps the LDL particle. Our model also explains how all known ligand-defective mutations in apoB100, including a newly discovered R3480W mutation in apoB100, cause defective receptor binding.}}, author = {{Boren, Jan and Ekström, Ulf and Ågren, Bo and Nilsson-Ehle, Peter and Innerarity, Thomas L}}, issn = {{1083-351X}}, language = {{eng}}, number = {{12}}, pages = {{9214--9218}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{The molecular mechanism for the genetic disorder familial defective apolipoprotein B100}}, url = {{http://dx.doi.org/10.1074/jbc.M008890200}}, doi = {{10.1074/jbc.M008890200}}, volume = {{276}}, year = {{2001}}, }