Prediction of reversible IgG1 aggregation occurring in a size exclusion chromatography column is enabled through a model based approach
Ojala, Frida LU ; Sellberg, Anton LU ; Budde Hansen, Thomas ; Broberg Hansen, Ernst and Nilsson, Bernt LU
(2015)
In Biotechnology Journal
10(11).
p.1814-1821
- Abstract
- One important aspect of antibody separation being studied today is aggregation, as this not only leads to a loss in yield, but aggregates can also be hazardous if injected in to the body. The aim of the study was to determine whether the methodology applied in the previous study could be used to predict the aggregation of a different batch of IgG1, and to model the aggregation occurring in a SEC column. Aggregation was found to be reversible. The equilibrium parameter was found to be 272 M(-1) and the reaction kinetic parameter 1.33·10(-5) s(-1) , both within the 95%percnt; confidence interval of the results obtained in the previous work. The effective diffusivities were estimated to be 1.45·10(-13) and 1.90·10(-14) m(2) /s for the... (More)
- One important aspect of antibody separation being studied today is aggregation, as this not only leads to a loss in yield, but aggregates can also be hazardous if injected in to the body. The aim of the study was to determine whether the methodology applied in the previous study could be used to predict the aggregation of a different batch of IgG1, and to model the aggregation occurring in a SEC column. Aggregation was found to be reversible. The equilibrium parameter was found to be 272 M(-1) and the reaction kinetic parameter 1.33·10(-5) s(-1) , both within the 95%percnt; confidence interval of the results obtained in the previous work. The effective diffusivities were estimated to be 1.45·10(-13) and 1.90·10(-14) m(2) /s for the monomers and dimers, respectively. Good agreement was found between the new model and the chromatograms obtained in the SEC experiments. The model was also able to predict the decrease of dimers due to the dilution and separation in the SEC column during long retention times. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7720666
- author
- Ojala, Frida
LU
; Sellberg, Anton
LU
; Budde Hansen, Thomas
; Broberg Hansen, Ernst
and Nilsson, Bernt
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Journal
- volume
- 10
- issue
- 11
- pages
- 1814 - 1821
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:26212800
- wos:000365129600017
- scopus:84947025358
- pmid:26212800
- ISSN
- 1860-6768
- DOI
- 10.1002/biot.201500160
- language
- English
- LU publication?
- yes
- id
- c5b8f31c-4a61-450c-9c0a-8929fffe903b (old id 7720666)
- date added to LUP
- 2016-04-01 10:51:25
- date last changed
- 2024-10-07 15:00:55
@article{c5b8f31c-4a61-450c-9c0a-8929fffe903b,
abstract = {{One important aspect of antibody separation being studied today is aggregation, as this not only leads to a loss in yield, but aggregates can also be hazardous if injected in to the body. The aim of the study was to determine whether the methodology applied in the previous study could be used to predict the aggregation of a different batch of IgG1, and to model the aggregation occurring in a SEC column. Aggregation was found to be reversible. The equilibrium parameter was found to be 272 M(-1) and the reaction kinetic parameter 1.33·10(-5) s(-1) , both within the 95%percnt; confidence interval of the results obtained in the previous work. The effective diffusivities were estimated to be 1.45·10(-13) and 1.90·10(-14) m(2) /s for the monomers and dimers, respectively. Good agreement was found between the new model and the chromatograms obtained in the SEC experiments. The model was also able to predict the decrease of dimers due to the dilution and separation in the SEC column during long retention times.}},
author = {{Ojala, Frida and Sellberg, Anton and Budde Hansen, Thomas and Broberg Hansen, Ernst and Nilsson, Bernt}},
issn = {{1860-6768}},
language = {{eng}},
number = {{11}},
pages = {{1814--1821}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Biotechnology Journal}},
title = {{Prediction of reversible IgG1 aggregation occurring in a size exclusion chromatography column is enabled through a model based approach}},
url = {{http://dx.doi.org/10.1002/biot.201500160}},
doi = {{10.1002/biot.201500160}},
volume = {{10}},
year = {{2015}},
}