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Identification of a strongly activating human anti-CD40 antibody that suppresses HIV-1 infection

Ellmark, Peter LU ; Andersson, Henrik; Abayneh, Sisay; Fenyö, Eva Maria LU and Borrebaeck, Carl LU (2008) In AIDS Research and Human Retroviruses 24(3). p.367-373
Abstract
We characterized the functional properties of a novel set of human anti-CD40 monoclonal antibodies originating from a human phage display library and identified an antibody that strongly activates cells via the CD40 receptor for potential use in HIV therapy. The anti-CD40 antibodies were converted from a single chain antibody fragment format (scFv) to an IgG format and produced in HEK293 cells, and the binding characteristics were evaluated. Next, their ability to (1) rescue a human B cell line from induced apoptosis, (2) stimulate B cell proliferation, and (3) block the CD40-CD40L interaction was determined. Finally, the most activating anti-CD40 antibody was tested for its ability to block HIV-1 infection in a monocyte-derived cell... (More)
We characterized the functional properties of a novel set of human anti-CD40 monoclonal antibodies originating from a human phage display library and identified an antibody that strongly activates cells via the CD40 receptor for potential use in HIV therapy. The anti-CD40 antibodies were converted from a single chain antibody fragment format (scFv) to an IgG format and produced in HEK293 cells, and the binding characteristics were evaluated. Next, their ability to (1) rescue a human B cell line from induced apoptosis, (2) stimulate B cell proliferation, and (3) block the CD40-CD40L interaction was determined. Finally, the most activating anti-CD40 antibody was tested for its ability to block HIV-1 infection in a monocyte-derived cell line.The different anti-CD40 antibodies, A24, B44, E30, F33, and A2-54, displayed a wide variety of binding and functional properties. In particular, B44 showed a very strong ability to activate normal human B cells and, in addition, did not block the CD40-CD40L interaction. This antibody was able to suppress HIV-1 infection in a human cell line (MonoMac 1) and may be a potential therapeutic candidate in HIV infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
AIDS Research and Human Retroviruses
volume
24
issue
3
pages
367 - 373
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:18327984
  • wos:000254735200004
  • scopus:41449108853
ISSN
1931-8405
DOI
10.1089/aid.2007.0215
language
English
LU publication?
yes
id
87b24ec5-819a-424e-a1a5-6bc8c510cc80 (old id 773591)
date added to LUP
2007-12-19 14:06:52
date last changed
2017-01-01 04:26:47
@article{87b24ec5-819a-424e-a1a5-6bc8c510cc80,
  abstract     = {We characterized the functional properties of a novel set of human anti-CD40 monoclonal antibodies originating from a human phage display library and identified an antibody that strongly activates cells via the CD40 receptor for potential use in HIV therapy. The anti-CD40 antibodies were converted from a single chain antibody fragment format (scFv) to an IgG format and produced in HEK293 cells, and the binding characteristics were evaluated. Next, their ability to (1) rescue a human B cell line from induced apoptosis, (2) stimulate B cell proliferation, and (3) block the CD40-CD40L interaction was determined. Finally, the most activating anti-CD40 antibody was tested for its ability to block HIV-1 infection in a monocyte-derived cell line.The different anti-CD40 antibodies, A24, B44, E30, F33, and A2-54, displayed a wide variety of binding and functional properties. In particular, B44 showed a very strong ability to activate normal human B cells and, in addition, did not block the CD40-CD40L interaction. This antibody was able to suppress HIV-1 infection in a human cell line (MonoMac 1) and may be a potential therapeutic candidate in HIV infection.},
  author       = {Ellmark, Peter and Andersson, Henrik and Abayneh, Sisay and Fenyö, Eva Maria and Borrebaeck, Carl},
  issn         = {1931-8405},
  language     = {eng},
  number       = {3},
  pages        = {367--373},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {AIDS Research and Human Retroviruses},
  title        = {Identification of a strongly activating human anti-CD40 antibody that suppresses HIV-1 infection},
  url          = {http://dx.doi.org/10.1089/aid.2007.0215},
  volume       = {24},
  year         = {2008},
}