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Low Rates of Transmitted Drug Resistance Among Newly Identified HIV-1 Seroconverters in Rural Rakai, Uganda

Reynolds, Steven J ; Ssempijja, Victor ; Galiwango, Ronald ; Ndyanabo, Anthony ; Nakigozi, Gertrude ; Lyagoba, Fred ; Nazziwa, Jamirah LU orcid ; Redd, Andrew ; Lamers, Susanna L and Gray, Ron , et al. (2017) In AIDS Research and Human Retroviruses 33(5). p.448-451
Abstract

We investigated the rate of transmitted drug resistance (TDR) among HIV-1 seroconverters identified from the Rakai Community Cohort Study (RCCS) survey, a population-based cohort in Rakai District, Uganda. Participants aged 15-49 are interviewed at study visits approximately every 12-18 months and provided a serological sample. Antiretroviral therapy (ART) has been provided free of charge since 2004. RCCS participants with documented negative HIV-1 serology between January 2011 and August 2012 and confirmed seroconversion between November 2012 and October 2013 were included in this analysis. Serum was genotyped for HIV drug resistance mutations in reverse transcriptase and protease genes. Mutations were classified according to the 2009... (More)

We investigated the rate of transmitted drug resistance (TDR) among HIV-1 seroconverters identified from the Rakai Community Cohort Study (RCCS) survey, a population-based cohort in Rakai District, Uganda. Participants aged 15-49 are interviewed at study visits approximately every 12-18 months and provided a serological sample. Antiretroviral therapy (ART) has been provided free of charge since 2004. RCCS participants with documented negative HIV-1 serology between January 2011 and August 2012 and confirmed seroconversion between November 2012 and October 2013 were included in this analysis. Serum was genotyped for HIV drug resistance mutations in reverse transcriptase and protease genes. Mutations were classified according to the 2009 World Health Organization surveillance of transmitted HIV-1 drug resistance update. Seventy-five (75) seroconverters were identified and genotyped. The mean age was 28 years (range 18-49) and the majority were male, n = 44 (58%). The HIV-1 subtype frequencies were A = 19 (25%), D = 44 (59%), C = 4 (5%), A/D recombinant = 5 (7%), and C/D recombinant = 3 (4%). The majority (72/75, 96%) of individuals were infected with wild-type virus with no evidence of TDR. Two individuals had a single non-nucleoside reverse transcriptase inhibitor mutation each, K101E and K103N, and one had a single protease inhibitor mutation, M46I. No mutations were identified involving nucleoside reverse transcriptase inhibitors. In conclusion, almost 10 years after the introduction of ART in rural Uganda, rates of TDR remain low. Ongoing surveillance for TDR remains an important public health priority and should be conducted among known seroconverters to estimate TDR.

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publishing date
type
Contribution to journal
publication status
published
keywords
Adolescent, Adult, Cohort Studies, Drug Resistance, Viral, Female, Genotyping Techniques, HIV Infections, HIV Protease, HIV Reverse Transcriptase, HIV-1, Humans, Male, Middle Aged, Prevalence, Rural Population, Uganda, Young Adult, Journal Article, Research Support, N.I.H., Extramural
in
AIDS Research and Human Retroviruses
volume
33
issue
5
pages
4 pages
publisher
Mary Ann Liebert, Inc.
external identifiers
  • pmid:27798967
  • scopus:85019162548
ISSN
1931-8405
DOI
10.1089/AID.2015.0370
language
English
LU publication?
no
id
77384d2d-bf49-43ac-a688-0d159440a202
date added to LUP
2018-03-22 14:04:36
date last changed
2024-02-13 17:28:37
@article{77384d2d-bf49-43ac-a688-0d159440a202,
  abstract     = {{<p>We investigated the rate of transmitted drug resistance (TDR) among HIV-1 seroconverters identified from the Rakai Community Cohort Study (RCCS) survey, a population-based cohort in Rakai District, Uganda. Participants aged 15-49 are interviewed at study visits approximately every 12-18 months and provided a serological sample. Antiretroviral therapy (ART) has been provided free of charge since 2004. RCCS participants with documented negative HIV-1 serology between January 2011 and August 2012 and confirmed seroconversion between November 2012 and October 2013 were included in this analysis. Serum was genotyped for HIV drug resistance mutations in reverse transcriptase and protease genes. Mutations were classified according to the 2009 World Health Organization surveillance of transmitted HIV-1 drug resistance update. Seventy-five (75) seroconverters were identified and genotyped. The mean age was 28 years (range 18-49) and the majority were male, n = 44 (58%). The HIV-1 subtype frequencies were A = 19 (25%), D = 44 (59%), C = 4 (5%), A/D recombinant = 5 (7%), and C/D recombinant = 3 (4%). The majority (72/75, 96%) of individuals were infected with wild-type virus with no evidence of TDR. Two individuals had a single non-nucleoside reverse transcriptase inhibitor mutation each, K101E and K103N, and one had a single protease inhibitor mutation, M46I. No mutations were identified involving nucleoside reverse transcriptase inhibitors. In conclusion, almost 10 years after the introduction of ART in rural Uganda, rates of TDR remain low. Ongoing surveillance for TDR remains an important public health priority and should be conducted among known seroconverters to estimate TDR.</p>}},
  author       = {{Reynolds, Steven J and Ssempijja, Victor and Galiwango, Ronald and Ndyanabo, Anthony and Nakigozi, Gertrude and Lyagoba, Fred and Nazziwa, Jamirah and Redd, Andrew and Lamers, Susanna L and Gray, Ron and Wawer, Maria and Serwadda, David and Quinn, Thomas C}},
  issn         = {{1931-8405}},
  keywords     = {{Adolescent; Adult; Cohort Studies; Drug Resistance, Viral; Female; Genotyping Techniques; HIV Infections; HIV Protease; HIV Reverse Transcriptase; HIV-1; Humans; Male; Middle Aged; Prevalence; Rural Population; Uganda; Young Adult; Journal Article; Research Support, N.I.H., Extramural}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{448--451}},
  publisher    = {{Mary Ann Liebert, Inc.}},
  series       = {{AIDS Research and Human Retroviruses}},
  title        = {{Low Rates of Transmitted Drug Resistance Among Newly Identified HIV-1 Seroconverters in Rural Rakai, Uganda}},
  url          = {{http://dx.doi.org/10.1089/AID.2015.0370}},
  doi          = {{10.1089/AID.2015.0370}},
  volume       = {{33}},
  year         = {{2017}},
}