Sensitivity of HIV-1 primary isolates to human anti-CD40 antibody-mediated suppression is related to co-receptor use
(2008) In AIDS Research and Human Retroviruses 24(3). p.447-452- Abstract
- The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric... (More)
- The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric receptors. In comparison, HIV-1 isolates with broader use of CCR5/CXCR4 chimeric receptors or with CXCR4 use were less sensitive to anti-CD40-induced suppression. The results indicate that HIV-1 replication is inhibited by human anti-CD40 monoclonal antibodies through the mechanism of CC-chemokine induction. This effect is thus restricted to HIV-1 isolates sensitive to inhibition by CC-chemokines. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/773857
- author
- Abayneh, Sisay A ; Ellmark, Peter LU ; Karlsson, Ulf LU ; Andersson, Henrik ; Borrebaeck, Carl LU ; Karlsson, Ingrid LU and Fenyö, Eva Maria LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- AIDS Research and Human Retroviruses
- volume
- 24
- issue
- 3
- pages
- 447 - 452
- publisher
- Mary Ann Liebert, Inc.
- external identifiers
-
- wos:000254735200014
- pmid:18373433
- scopus:41449100085
- pmid:18373433
- ISSN
- 1931-8405
- DOI
- 10.1089/aid.2007.0216
- language
- English
- LU publication?
- yes
- id
- 445aeff0-5d77-4f11-8294-52d320d8b1ed (old id 773857)
- date added to LUP
- 2016-04-01 12:34:39
- date last changed
- 2022-01-27 07:00:42
@article{445aeff0-5d77-4f11-8294-52d320d8b1ed, abstract = {{The effect of CD40 ligation on infection by HIV-1 primary isolates with different R5 phenotypes was evaluated with a novel set of anti-CD40 monoclonal antibodies originating from a human phage display library. Five human monoclonal anti-CD40 antibodies of IgG1 subtype characterized by the ability to activate B cells via CD40 were tested for induction of the CC-chemokines RANTES and MIP-1alpha and inhibition of HIV-1 replication in primary monocyte-derived macrophages (MDM). All activating anti-CD40 antibodies were able to induce CC-chemokines in MDM. We chose the most potent antibody, clone B44, for further experiments. This antibody had a suppressive effect on HIV-1 isolates of the R5 phenotype with limited use of CCR5/CXCR4 chimeric receptors. In comparison, HIV-1 isolates with broader use of CCR5/CXCR4 chimeric receptors or with CXCR4 use were less sensitive to anti-CD40-induced suppression. The results indicate that HIV-1 replication is inhibited by human anti-CD40 monoclonal antibodies through the mechanism of CC-chemokine induction. This effect is thus restricted to HIV-1 isolates sensitive to inhibition by CC-chemokines.}}, author = {{Abayneh, Sisay A and Ellmark, Peter and Karlsson, Ulf and Andersson, Henrik and Borrebaeck, Carl and Karlsson, Ingrid and Fenyö, Eva Maria}}, issn = {{1931-8405}}, language = {{eng}}, number = {{3}}, pages = {{447--452}}, publisher = {{Mary Ann Liebert, Inc.}}, series = {{AIDS Research and Human Retroviruses}}, title = {{Sensitivity of HIV-1 primary isolates to human anti-CD40 antibody-mediated suppression is related to co-receptor use}}, url = {{http://dx.doi.org/10.1089/aid.2007.0216}}, doi = {{10.1089/aid.2007.0216}}, volume = {{24}}, year = {{2008}}, }