In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry.
(2015) In Cell Reports 12(3). p.474-481- Abstract
- The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are... (More)
- The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7749788
- author
- Torper, Olof
LU
; Rylander, Daniella
LU
; Pereira, Maria J M LU ; Lau, Shong LU ; Cardoso, Tiago LU ; Grealish, Shane LU and Parmar, Malin LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Reports
- volume
- 12
- issue
- 3
- pages
- 474 - 481
- publisher
- Cell Press
- external identifiers
-
- pmid:26166567
- wos:000358324900013
- scopus:84937523716
- pmid:26166567
- ISSN
- 2211-1247
- DOI
- 10.1016/j.celrep.2015.06.040
- project
- Cell reprogramming: a new method for generating functional neurons
- language
- English
- LU publication?
- yes
- id
- 84e9e24b-294d-4ece-8146-1aa0e4f33c32 (old id 7749788)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26166567?dopt=Abstract
- date added to LUP
- 2016-04-01 12:59:42
- date last changed
- 2024-06-05 06:09:18
@article{84e9e24b-294d-4ece-8146-1aa0e4f33c32, abstract = {{The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain.}}, author = {{Torper, Olof and Rylander, Daniella and Pereira, Maria J M and Lau, Shong and Cardoso, Tiago and Grealish, Shane and Parmar, Malin}}, issn = {{2211-1247}}, language = {{eng}}, number = {{3}}, pages = {{474--481}}, publisher = {{Cell Press}}, series = {{Cell Reports}}, title = {{In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry.}}, url = {{http://dx.doi.org/10.1016/j.celrep.2015.06.040}}, doi = {{10.1016/j.celrep.2015.06.040}}, volume = {{12}}, year = {{2015}}, }