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In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry.

Torper, Olof LU ; Rylander, Daniella LU ; Pereira, Maria J M LU ; Lau, Shong LU ; Cardoso, Tiago LU ; Grealish, Shane LU and Parmar, Malin LU (2015) In Cell Reports 12(3). p.474-481
Abstract
The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are... (More)
The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Reports
volume
12
issue
3
pages
474 - 481
publisher
Cell Press
external identifiers
  • pmid:26166567
  • wos:000358324900013
  • scopus:84937523716
ISSN
2211-1247
DOI
10.1016/j.celrep.2015.06.040
language
English
LU publication?
yes
id
84e9e24b-294d-4ece-8146-1aa0e4f33c32 (old id 7749788)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26166567?dopt=Abstract
date added to LUP
2015-08-08 00:39:39
date last changed
2017-11-19 03:42:14
@article{84e9e24b-294d-4ece-8146-1aa0e4f33c32,
  abstract     = {The possibility of directly converting non-neuronal cells into neurons in situ in the brain would open therapeutic avenues aimed at repairing the brain after injury or degenerative disease. We have developed an adeno-associated virus (AAV)-based reporter system that allows selective GFP labeling of reprogrammed neurons. In this system, GFP is turned on only in reprogrammed neurons where it is stable and maintained for long time periods, allowing for histological and functional characterization of mature neurons. When combined with a modified rabies virus-based trans-synaptic tracing methodology, the system allows mapping of 3D circuitry integration into local and distal brain regions and shows that the newly reprogrammed neurons are integrated into host brain.},
  author       = {Torper, Olof and Rylander, Daniella and Pereira, Maria J M and Lau, Shong and Cardoso, Tiago and Grealish, Shane and Parmar, Malin},
  issn         = {2211-1247},
  language     = {eng},
  number       = {3},
  pages        = {474--481},
  publisher    = {Cell Press},
  series       = {Cell Reports},
  title        = {In Vivo Reprogramming of Striatal NG2 Glia into Functional Neurons that Integrate into Local Host Circuitry.},
  url          = {http://dx.doi.org/10.1016/j.celrep.2015.06.040},
  volume       = {12},
  year         = {2015},
}