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Promoter-mediated, dexamethasone-induced increase in cystatin C production by HeLa cells

Bjarnadóttir, M LU ; Grubb, A LU orcid and Olafsson, I (1995) In Scandinavian Journal of Clinical and Laboratory Investigation 55(7). p.617-623
Abstract

Cystatin C, an efficient inhibitor of cysteine proteinases, is present in all investigated human extracellular fluids. Dexamethasone caused a significant and dose-dependent increase in the cystatin C secretion of cultivated HeLa cells up to a maximal increase of 80% at 10(-6) mol l-1 dexamethasone. Increased production of cystatin C was also observed at lower concentrations, suggesting that glucocorticoids might play a physiological role in the production of cystatin C. The effect of dexamethasone on the cystatin C gene expression was also studied in a transient transfection expression system using chimeric plasmid constructs of the cystatin C gene promoter (positions -2 to -1084) coupled to the structural gene for human growth hormone... (More)

Cystatin C, an efficient inhibitor of cysteine proteinases, is present in all investigated human extracellular fluids. Dexamethasone caused a significant and dose-dependent increase in the cystatin C secretion of cultivated HeLa cells up to a maximal increase of 80% at 10(-6) mol l-1 dexamethasone. Increased production of cystatin C was also observed at lower concentrations, suggesting that glucocorticoids might play a physiological role in the production of cystatin C. The effect of dexamethasone on the cystatin C gene expression was also studied in a transient transfection expression system using chimeric plasmid constructs of the cystatin C gene promoter (positions -2 to -1084) coupled to the structural gene for human growth hormone (hGH). In this system, a small, but statistically significant, increase in hGH secretion was also observed upon dexamethasone treatment, suggesting that the glucocorticoid-induced increase in secretion of cystatin C is due to a promoter-mediated increase in transcription of the cystatin C gene.

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author
; and
publishing date
type
Contribution to journal
publication status
published
keywords
Cystatin C, Cystatins/genetics, Cysteine Proteinase Inhibitors/genetics, Dexamethasone/pharmacology, Dose-Response Relationship, Drug, Gene Expression Regulation/drug effects, Glucocorticoids/pharmacology, Growth Hormone/biosynthesis, HeLa Cells/drug effects, Humans, Promoter Regions, Genetic/drug effects, Time Factors, Transcription, Genetic/genetics, Transfection
in
Scandinavian Journal of Clinical and Laboratory Investigation
volume
55
issue
7
pages
617 - 623
publisher
Informa Healthcare
external identifiers
  • scopus:0028859421
  • pmid:8633186
ISSN
0036-5513
DOI
10.3109/00365519509110261
language
English
LU publication?
no
id
775a8ec5-e511-4806-8d76-64af7bb9af02
date added to LUP
2021-10-28 13:57:09
date last changed
2024-04-05 15:24:35
@article{775a8ec5-e511-4806-8d76-64af7bb9af02,
  abstract     = {{<p>Cystatin C, an efficient inhibitor of cysteine proteinases, is present in all investigated human extracellular fluids. Dexamethasone caused a significant and dose-dependent increase in the cystatin C secretion of cultivated HeLa cells up to a maximal increase of 80% at 10(-6) mol l-1 dexamethasone. Increased production of cystatin C was also observed at lower concentrations, suggesting that glucocorticoids might play a physiological role in the production of cystatin C. The effect of dexamethasone on the cystatin C gene expression was also studied in a transient transfection expression system using chimeric plasmid constructs of the cystatin C gene promoter (positions -2 to -1084) coupled to the structural gene for human growth hormone (hGH). In this system, a small, but statistically significant, increase in hGH secretion was also observed upon dexamethasone treatment, suggesting that the glucocorticoid-induced increase in secretion of cystatin C is due to a promoter-mediated increase in transcription of the cystatin C gene.</p>}},
  author       = {{Bjarnadóttir, M and Grubb, A and Olafsson, I}},
  issn         = {{0036-5513}},
  keywords     = {{Cystatin C; Cystatins/genetics; Cysteine Proteinase Inhibitors/genetics; Dexamethasone/pharmacology; Dose-Response Relationship, Drug; Gene Expression Regulation/drug effects; Glucocorticoids/pharmacology; Growth Hormone/biosynthesis; HeLa Cells/drug effects; Humans; Promoter Regions, Genetic/drug effects; Time Factors; Transcription, Genetic/genetics; Transfection}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{617--623}},
  publisher    = {{Informa Healthcare}},
  series       = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
  title        = {{Promoter-mediated, dexamethasone-induced increase in cystatin C production by HeLa cells}},
  url          = {{http://dx.doi.org/10.3109/00365519509110261}},
  doi          = {{10.3109/00365519509110261}},
  volume       = {{55}},
  year         = {{1995}},
}