Advanced

Streptococcus pyogenes Infection and the Human Proteome with a Special Focus on the Immunoglobulin G-cleaving Enzyme IdeS

Karlsson, Christofer A.Q. LU ; Järnum, Sofia LU ; Winstedt, Lena LU ; Kjellman, Christian LU ; Björck, Lars LU ; Linder, Adam LU and Malmström, Johan A. LU (2018) In Molecular and Cellular Proteomics 17(6). p.1097-1111
Abstract

Infectious diseases are characterized by a complex interplay between host and pathogen, but how these interactions impact the host proteome is unclear. Here we applied a combined mass spectrometry-based proteomics strategy to investigate how the human proteome is transiently modified by the pathogen Streptococcus pyogenes, with a particular focus on bacterial cleavage of IgG in vivo. In invasive diseases, S. pyogenes evokes a massive host response in blood, whereas superficial diseases are characterized by a local leakage of several blood plasma proteins at the site of infection including IgG. S. pyogenes produces IdeS, a protease cleaving IgG in the lower hinge region and we find highly effective IdeS-cleavage of IgG in samples from... (More)

Infectious diseases are characterized by a complex interplay between host and pathogen, but how these interactions impact the host proteome is unclear. Here we applied a combined mass spectrometry-based proteomics strategy to investigate how the human proteome is transiently modified by the pathogen Streptococcus pyogenes, with a particular focus on bacterial cleavage of IgG in vivo. In invasive diseases, S. pyogenes evokes a massive host response in blood, whereas superficial diseases are characterized by a local leakage of several blood plasma proteins at the site of infection including IgG. S. pyogenes produces IdeS, a protease cleaving IgG in the lower hinge region and we find highly effective IdeS-cleavage of IgG in samples from local IgG poor microenvironments. The results show that IdeS contributes to the adaptation of S. pyogenes to its normal ecological niches. Additionally, the work identifies novel clinical opportunities for in vivo pathogen detection.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Proteomics
volume
17
issue
6
pages
1097 - 1111
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • scopus:85048154422
ISSN
1535-9476
DOI
10.1074/mcp.RA117.000525
language
English
LU publication?
yes
id
775d3c81-49ab-4dd4-b146-64e9cce27780
date added to LUP
2018-06-20 16:38:33
date last changed
2019-09-17 04:34:33
@article{775d3c81-49ab-4dd4-b146-64e9cce27780,
  abstract     = {<p>Infectious diseases are characterized by a complex interplay between host and pathogen, but how these interactions impact the host proteome is unclear. Here we applied a combined mass spectrometry-based proteomics strategy to investigate how the human proteome is transiently modified by the pathogen Streptococcus pyogenes, with a particular focus on bacterial cleavage of IgG in vivo. In invasive diseases, S. pyogenes evokes a massive host response in blood, whereas superficial diseases are characterized by a local leakage of several blood plasma proteins at the site of infection including IgG. S. pyogenes produces IdeS, a protease cleaving IgG in the lower hinge region and we find highly effective IdeS-cleavage of IgG in samples from local IgG poor microenvironments. The results show that IdeS contributes to the adaptation of S. pyogenes to its normal ecological niches. Additionally, the work identifies novel clinical opportunities for in vivo pathogen detection.</p>},
  author       = {Karlsson, Christofer A.Q. and Järnum, Sofia and Winstedt, Lena and Kjellman, Christian and Björck, Lars and Linder, Adam and Malmström, Johan A.},
  issn         = {1535-9476},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {1097--1111},
  publisher    = {American Society for Biochemistry and Molecular Biology},
  series       = {Molecular and Cellular Proteomics},
  title        = {Streptococcus pyogenes Infection and the Human Proteome with a Special Focus on the Immunoglobulin G-cleaving Enzyme IdeS},
  url          = {http://dx.doi.org/10.1074/mcp.RA117.000525},
  volume       = {17},
  year         = {2018},
}