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Cellular nutrient content measured with the nuclear microprobe and toxins produced by Dinophysis norvegica (Dinophyceae) from the Trondheim fjord (Norway)

Blanco, Eva Perez; Karlsson, Chatarina; Pallon, Jan LU ; Yasumoto, Takeshi and Graneli, Edna LU (2015) In Aquatic Microbial Ecology 75(3). p.259-269
Abstract
Dinophysis norvegica is a commonly occurring dinoflagellate species and a producer of diarrhetic shellfish poisons. Natural samples were collected from Trondheim fjord, Norway, to analyse nutrient (O, C, N, P) and toxin (dinophysitoxins [DXTs], okadaic acid [OA], pectenotoxins [PTXs]) content in D. norvegica cells. Nutrient and toxin analyses were also carried out on cells grown under nutrient-sufficient and nutrient-deficient conditions to determine how intracellular nutrient and toxin content varied under different nutrient availability conditions. Nutrient analyses were conducted using nuclear microprobe techniques that can accurately analyse single cells, and toxin analyses were carried out using liquid chromatography and mass... (More)
Dinophysis norvegica is a commonly occurring dinoflagellate species and a producer of diarrhetic shellfish poisons. Natural samples were collected from Trondheim fjord, Norway, to analyse nutrient (O, C, N, P) and toxin (dinophysitoxins [DXTs], okadaic acid [OA], pectenotoxins [PTXs]) content in D. norvegica cells. Nutrient and toxin analyses were also carried out on cells grown under nutrient-sufficient and nutrient-deficient conditions to determine how intracellular nutrient and toxin content varied under different nutrient availability conditions. Nutrient analyses were conducted using nuclear microprobe techniques that can accurately analyse single cells, and toxin analyses were carried out using liquid chromatography and mass spectroscopy. The intracellular carbon, nitrogen and phosphorus content in individual cells varied greatly, and intracellular C:N:P ratios showed that the cells were both N- and P-deficient when compared to the Redfield ratio. The ideal N:P ratio in the media for D. norvegica was found to be below the Redfield ratio, but intracellular ratios did not show a clear relationship with those in the media. N:P ratios of D. norvegica were higher than expected, which is likely due to their phagotrophy on zooplankon. The highest toxin values found were traces of PTX2, 24.72 pg PTX2SA cell(-1), 2.19 pg DTX1 cell(-1), and 1.01 pg OA cell(-1). However, we found no clear relationship between the content of intracellular nutrients and toxins. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Dinophysis, Diarrhetic shellfish poisoning, Elemental cellular content, Mixotrophy, Nuclear microprobe, Nutrient ratios, Nutrients, Trondheim, fjord
in
Aquatic Microbial Ecology
volume
75
issue
3
pages
259 - 269
publisher
Inter-Research
external identifiers
  • wos:000358512400006
  • scopus:84937910336
ISSN
0948-3055
DOI
10.3354/ame01762
language
English
LU publication?
yes
id
680f7e65-4e49-4dec-a770-9de522822bfe (old id 7773520)
date added to LUP
2015-09-18 12:02:57
date last changed
2017-07-09 04:04:09
@article{680f7e65-4e49-4dec-a770-9de522822bfe,
  abstract     = {Dinophysis norvegica is a commonly occurring dinoflagellate species and a producer of diarrhetic shellfish poisons. Natural samples were collected from Trondheim fjord, Norway, to analyse nutrient (O, C, N, P) and toxin (dinophysitoxins [DXTs], okadaic acid [OA], pectenotoxins [PTXs]) content in D. norvegica cells. Nutrient and toxin analyses were also carried out on cells grown under nutrient-sufficient and nutrient-deficient conditions to determine how intracellular nutrient and toxin content varied under different nutrient availability conditions. Nutrient analyses were conducted using nuclear microprobe techniques that can accurately analyse single cells, and toxin analyses were carried out using liquid chromatography and mass spectroscopy. The intracellular carbon, nitrogen and phosphorus content in individual cells varied greatly, and intracellular C:N:P ratios showed that the cells were both N- and P-deficient when compared to the Redfield ratio. The ideal N:P ratio in the media for D. norvegica was found to be below the Redfield ratio, but intracellular ratios did not show a clear relationship with those in the media. N:P ratios of D. norvegica were higher than expected, which is likely due to their phagotrophy on zooplankon. The highest toxin values found were traces of PTX2, 24.72 pg PTX2SA cell(-1), 2.19 pg DTX1 cell(-1), and 1.01 pg OA cell(-1). However, we found no clear relationship between the content of intracellular nutrients and toxins.},
  author       = {Blanco, Eva Perez and Karlsson, Chatarina and Pallon, Jan and Yasumoto, Takeshi and Graneli, Edna},
  issn         = {0948-3055},
  keyword      = {Dinophysis,Diarrhetic shellfish poisoning,Elemental cellular content,Mixotrophy,Nuclear microprobe,Nutrient ratios,Nutrients,Trondheim,fjord},
  language     = {eng},
  number       = {3},
  pages        = {259--269},
  publisher    = {Inter-Research},
  series       = {Aquatic Microbial Ecology},
  title        = {Cellular nutrient content measured with the nuclear microprobe and toxins produced by Dinophysis norvegica (Dinophyceae) from the Trondheim fjord (Norway)},
  url          = {http://dx.doi.org/10.3354/ame01762},
  volume       = {75},
  year         = {2015},
}