Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells
(2017) In Farmacia 65(2). p.214-218- Abstract
Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol... (More)
Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.
(Less)
- author
- organization
- publishing date
- 2017
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cox-2, Mir-146a-5p, Raw264.7 cells, Resveratrol, Vitis heyneana roem. & schult
- in
- Farmacia
- volume
- 65
- issue
- 2
- pages
- 5 pages
- publisher
- Romanian Society for Pharmaceutical Sciences
- external identifiers
-
- scopus:85018377174
- ISSN
- 0014-8237
- language
- English
- LU publication?
- yes
- id
- 77895655-ae35-45c8-9d07-ca399c6bae42
- date added to LUP
- 2017-05-23 13:09:37
- date last changed
- 2022-02-14 19:35:40
@article{77895655-ae35-45c8-9d07-ca399c6bae42, abstract = {{<p>Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.</p>}}, author = {{Dao, Trong Tuan and Ha, Do Thi and Hien Tran, Thi and Binh, Bui Thi and Phuong, Tran Thi and Long, Phung Thanh and Thu, Nguyen Bich and Que, Do Thi Nguyet and Khoi, Nguyen Minh and Dung, Le Viet and Ngoc, Tran Minh}}, issn = {{0014-8237}}, keywords = {{Cox-2; Mir-146a-5p; Raw264.7 cells; Resveratrol; Vitis heyneana roem. & schult}}, language = {{eng}}, number = {{2}}, pages = {{214--218}}, publisher = {{Romanian Society for Pharmaceutical Sciences}}, series = {{Farmacia}}, title = {{Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells}}, volume = {{65}}, year = {{2017}}, }