Advanced

Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells

Dao, Trong Tuan; Ha, Do Thi; Hien Tran, Thi LU ; Binh, Bui Thi; Phuong, Tran Thi; Long, Phung Thanh; Thu, Nguyen Bich; Que, Do Thi Nguyet; Khoi, Nguyen Minh and Dung, Le Viet, et al. (2017) In Farmacia 65(2). p.214-218
Abstract

Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol... (More)

Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.

(Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cox-2, Mir-146a-5p, Raw264.7 cells, Resveratrol, Vitis heyneana roem. & schult
in
Farmacia
volume
65
issue
2
pages
5 pages
external identifiers
  • scopus:85018377174
ISSN
0014-8237
language
English
LU publication?
yes
id
77895655-ae35-45c8-9d07-ca399c6bae42
date added to LUP
2017-05-23 13:09:37
date last changed
2017-05-23 13:09:37
@article{77895655-ae35-45c8-9d07-ca399c6bae42,
  abstract     = {<p>Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. &amp; Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.</p>},
  author       = {Dao, Trong Tuan and Ha, Do Thi and Hien Tran, Thi and Binh, Bui Thi and Phuong, Tran Thi and Long, Phung Thanh and Thu, Nguyen Bich and Que, Do Thi Nguyet and Khoi, Nguyen Minh and Dung, Le Viet and Ngoc, Tran Minh},
  issn         = {0014-8237},
  keyword      = {Cox-2,Mir-146a-5p,Raw264.7 cells,Resveratrol,Vitis heyneana roem. & schult},
  language     = {eng},
  number       = {2},
  pages        = {214--218},
  series       = {Farmacia},
  title        = {Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells},
  volume       = {65},
  year         = {2017},
}