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Steroid hormone measurements from different types of assays in relation to body mass index and breast cancer risk in postmenopausal women: Reanalysis of eighteen prospective studies

Key, T. J.; Appleby, P. N.; Reeves, G. K.; Travis, R. C.; Brinton, L. A.; Dallal, C. M.; Helzlsouer, K. J.; Hoffman-Bolton, J.; Visvanathan, K. and Dorgan, J. F., et al. (2015) In Steroids 99. p.49-55
Abstract
Epidemiological studies have examined breast cancer risk in relation to sex hormone concentrations measured by different methods: "extraction" immunoassays (with prior purification by organic solvent extraction, with or without column chromatography), "direct" immunoassays (no prior extraction or column chromatography), and more recently with mass spectrometry-based assays. We describe the associations of estradiol, estrone and testosterone with both body mass index and breast cancer risk in postmenopausal women according to assay method, using data from a collaborative pooled analysis of 18 prospective studies. In general, hormone concentrations were highest in studies that used direct assays and lowest in studies that used mass... (More)
Epidemiological studies have examined breast cancer risk in relation to sex hormone concentrations measured by different methods: "extraction" immunoassays (with prior purification by organic solvent extraction, with or without column chromatography), "direct" immunoassays (no prior extraction or column chromatography), and more recently with mass spectrometry-based assays. We describe the associations of estradiol, estrone and testosterone with both body mass index and breast cancer risk in postmenopausal women according to assay method, using data from a collaborative pooled analysis of 18 prospective studies. In general, hormone concentrations were highest in studies that used direct assays and lowest in studies that used mass spectrometry-based assays. Estradiol and estrone were strongly positively associated with body mass index, regardless of the assay method; testosterone was positively associated with body mass index for direct assays, but less clearly for extraction assays, and there were few data for mass spectrometry assays. The correlations of estradiol with body mass index, estrone and testosterone were lower for direct assays than for extraction and mass spectrometry assays, suggesting that the estimates from the direct assays were less precise. For breast cancer risk, all three hormones were strongly positively associated with risk regardless of assay method (except for testosterone by mass spectrometry where there were few data), with no statistically significant differences in the trends, but differences may emerge as new data accumulate. Future epidemiological and clinical research studies should continue to use the most accurate assays that are feasible within the design characteristics of each study. (Less)
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@article{15b38a34-114a-492a-a053-3c96fffaf2cf,
  abstract     = {Epidemiological studies have examined breast cancer risk in relation to sex hormone concentrations measured by different methods: "extraction" immunoassays (with prior purification by organic solvent extraction, with or without column chromatography), "direct" immunoassays (no prior extraction or column chromatography), and more recently with mass spectrometry-based assays. We describe the associations of estradiol, estrone and testosterone with both body mass index and breast cancer risk in postmenopausal women according to assay method, using data from a collaborative pooled analysis of 18 prospective studies. In general, hormone concentrations were highest in studies that used direct assays and lowest in studies that used mass spectrometry-based assays. Estradiol and estrone were strongly positively associated with body mass index, regardless of the assay method; testosterone was positively associated with body mass index for direct assays, but less clearly for extraction assays, and there were few data for mass spectrometry assays. The correlations of estradiol with body mass index, estrone and testosterone were lower for direct assays than for extraction and mass spectrometry assays, suggesting that the estimates from the direct assays were less precise. For breast cancer risk, all three hormones were strongly positively associated with risk regardless of assay method (except for testosterone by mass spectrometry where there were few data), with no statistically significant differences in the trends, but differences may emerge as new data accumulate. Future epidemiological and clinical research studies should continue to use the most accurate assays that are feasible within the design characteristics of each study.},
  author       = {Key, T. J. and Appleby, P. N. and Reeves, G. K. and Travis, R. C. and Brinton, L. A. and Dallal, C. M. and Helzlsouer, K. J. and Hoffman-Bolton, J. and Visvanathan, K. and Dorgan, J. F. and Falk, R. T. and Gapstur, S. M. and Gaudet, M. M. and Kaaks, R. and Riboli, E. and Rinaldi, S. and Key, T. and Manjer, Jonas and Hallmans, G. and Giles, G. G. and Le Marchand, L. and Kolonel, L. N. and Henderson, B. E. and Tworoger, S. S. and Hankinson, S. E. and Zeleniuch-Jacquotte, A. and Koenig, K. and Krogh, V. and Sieri, S. and Muti, P. and Ziegler, R. G. and Schairer, C. and Fuhrman, B. J. and Barrett-Connor, E. and Laughlin, G. A. and Grant, E. J. and Cologne, J. and Ohishi, W. and Hida, A. and Cauley, J. A. and Fourkala, E.-O. and Rohan, T. E. and Strickler, H. D. and Gunter, M. J.},
  issn         = {0039-128X},
  keyword      = {Breast cancer,Estradiol,Body mass index,Extraction immunoassay,Direct immunoassay,Mass spectrometry},
  language     = {eng},
  pages        = {49--55},
  publisher    = {Elsevier},
  series       = {Steroids},
  title        = {Steroid hormone measurements from different types of assays in relation to body mass index and breast cancer risk in postmenopausal women: Reanalysis of eighteen prospective studies},
  url          = {http://dx.doi.org/10.1016/j.steroids.2014.09.001},
  volume       = {99},
  year         = {2015},
}