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Plaque-associated lipids in Alzheimer's diseased brain tissue visualized by nonlinear microscopy.

Kiskis, Juris; Fink, Helen; Nyberg, Lena; Thyr, Jacob; Li, Jia-Yi LU and Enejder, Annika (2015) In Scientific Reports 5.
Abstract
By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain tissues, we show evidence of lipid deposits co-localizing with fibrillar β-amyloid (Aβ) plaques. Two lipid morphologies can be observed; lamellar structures and coalescing macro-aggregates of sub-micron sizes to ~25 μm. No significant lipid deposits were observed in non-fibrillar, diffuse plaques identified by Aβ immuno-staining. CARS microscopy of unlabeled samples confirms the lamellar and macro-aggregate lipid morphologies. The composition of the plaques was analyzed by CARS microspectroscopy and Raman microscopy; vibrational signatures of lipids with long acyl chains co-localize with... (More)
By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain tissues, we show evidence of lipid deposits co-localizing with fibrillar β-amyloid (Aβ) plaques. Two lipid morphologies can be observed; lamellar structures and coalescing macro-aggregates of sub-micron sizes to ~25 μm. No significant lipid deposits were observed in non-fibrillar, diffuse plaques identified by Aβ immuno-staining. CARS microscopy of unlabeled samples confirms the lamellar and macro-aggregate lipid morphologies. The composition of the plaques was analyzed by CARS microspectroscopy and Raman microscopy; vibrational signatures of lipids with long acyl chains co-localize with the β-sheet vibrations. The lipid fluidity was evaluated from the CARS spectra, illustrating that the lipid composition/organization varies throughout the plaques. Altogether this indicates close amyloid-lipid interplay in fibrillar Aβ plaques, rendering them more dynamic compositions than previously believed and, hence, potential sources of toxic oligomers. (Less)
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organization
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type
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publication status
published
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in
Scientific Reports
volume
5
publisher
Nature Publishing Group
external identifiers
  • wos:000360144800001
  • pmid:26311128
  • scopus:84940510248
ISSN
2045-2322
DOI
10.1038/srep13489
language
English
LU publication?
yes
id
e75a5a09-f7eb-421f-8e24-1af2fff6d6a2 (old id 7834335)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26311128?dopt=Abstract
date added to LUP
2015-09-07 20:42:09
date last changed
2017-09-17 06:53:18
@article{e75a5a09-f7eb-421f-8e24-1af2fff6d6a2,
  abstract     = {By simultaneous coherent anti-Stokes Raman scattering (CARS) and 2-photon fluorescence microscopy of Thioflavin-S stained Alzheimer´s diseased human brain tissues, we show evidence of lipid deposits co-localizing with fibrillar β-amyloid (Aβ) plaques. Two lipid morphologies can be observed; lamellar structures and coalescing macro-aggregates of sub-micron sizes to ~25 μm. No significant lipid deposits were observed in non-fibrillar, diffuse plaques identified by Aβ immuno-staining. CARS microscopy of unlabeled samples confirms the lamellar and macro-aggregate lipid morphologies. The composition of the plaques was analyzed by CARS microspectroscopy and Raman microscopy; vibrational signatures of lipids with long acyl chains co-localize with the β-sheet vibrations. The lipid fluidity was evaluated from the CARS spectra, illustrating that the lipid composition/organization varies throughout the plaques. Altogether this indicates close amyloid-lipid interplay in fibrillar Aβ plaques, rendering them more dynamic compositions than previously believed and, hence, potential sources of toxic oligomers.},
  articleno    = {13489},
  author       = {Kiskis, Juris and Fink, Helen and Nyberg, Lena and Thyr, Jacob and Li, Jia-Yi and Enejder, Annika},
  issn         = {2045-2322},
  language     = {eng},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Plaque-associated lipids in Alzheimer's diseased brain tissue visualized by nonlinear microscopy.},
  url          = {http://dx.doi.org/10.1038/srep13489},
  volume       = {5},
  year         = {2015},
}