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Genetic linkage facilitates cloning of Ert-m regulating plant architecture in barley and identified a strong candidate of Ant1 involved in anthocyanin biosynthesis.

Zakhrabekova, Shakhira LU ; Dockter, Christoph; Ahmann, Katharina; Braumann, Ilka; Gough, Simon P; Wendt, Toni; Lundqvist, Udda; Mascher, Martin; Stein, Nils and Hansson, Mats LU (2015) In Plant Molecular Biology 88(6). p.609-626
Abstract
The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. We used a near-isogenic line of the cultivar Bowman, BW316 (ert-m.34), to create four F2-mapping populations by crosses to the barley cultivars Barke, Morex, Bowman and Quench. We phenotyped and genotyped 460 plants, allowing the ert-m mutation to be mapped to an interval of 4.7 cM on the short arm of barley chromosome 7H. Bioinformatic searches identified 21 candidate gene models in the mapped region. One gene was orthologous... (More)
The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. We used a near-isogenic line of the cultivar Bowman, BW316 (ert-m.34), to create four F2-mapping populations by crosses to the barley cultivars Barke, Morex, Bowman and Quench. We phenotyped and genotyped 460 plants, allowing the ert-m mutation to be mapped to an interval of 4.7 cM on the short arm of barley chromosome 7H. Bioinformatic searches identified 21 candidate gene models in the mapped region. One gene was orthologous to a regulator of Arabidopsis thaliana plant architecture, ERECTA, encoding a leucine-rich repeat receptor-like kinase. Sequencing of HvERECTA in barley ert-m mutant accessions identified severe DNA changes in 15 mutants, including full gene deletions in ert-m.40 and ert-m.64. Both deletions, additionally causing anthocyanin deficiency, were found to stretch over a large region including two putative candidate genes for the anthocyanin biosynthesis locus Ant1. Analyses of ert-m and ant1 single- and double-deletion mutants suggest Ant1 as a closely linked gene encoding a R2R3 myeloblastosis transcription factor. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Plant Molecular Biology
volume
88
issue
6
pages
609 - 626
publisher
Kluwer
external identifiers
  • pmid:26228300
  • wos:000358943100007
  • scopus:84938518876
ISSN
1573-5028
DOI
10.1007/s11103-015-0350-x
language
English
LU publication?
yes
id
f7074efd-a444-4578-b961-db061bd5c3ee (old id 7839367)
date added to LUP
2015-09-11 13:15:16
date last changed
2017-01-01 03:00:14
@article{f7074efd-a444-4578-b961-db061bd5c3ee,
  abstract     = {The erectoides-m anthocyanin-less 1 (ert-m ant1) double mutants are among the very few examples of induced double mutants in barley. From phenotypic observations of mutant plants it is known that the Ert-m gene product regulates plant architecture whereas the Ant1 gene product is involved in anthocyanin biosynthesis. We used a near-isogenic line of the cultivar Bowman, BW316 (ert-m.34), to create four F2-mapping populations by crosses to the barley cultivars Barke, Morex, Bowman and Quench. We phenotyped and genotyped 460 plants, allowing the ert-m mutation to be mapped to an interval of 4.7 cM on the short arm of barley chromosome 7H. Bioinformatic searches identified 21 candidate gene models in the mapped region. One gene was orthologous to a regulator of Arabidopsis thaliana plant architecture, ERECTA, encoding a leucine-rich repeat receptor-like kinase. Sequencing of HvERECTA in barley ert-m mutant accessions identified severe DNA changes in 15 mutants, including full gene deletions in ert-m.40 and ert-m.64. Both deletions, additionally causing anthocyanin deficiency, were found to stretch over a large region including two putative candidate genes for the anthocyanin biosynthesis locus Ant1. Analyses of ert-m and ant1 single- and double-deletion mutants suggest Ant1 as a closely linked gene encoding a R2R3 myeloblastosis transcription factor.},
  author       = {Zakhrabekova, Shakhira and Dockter, Christoph and Ahmann, Katharina and Braumann, Ilka and Gough, Simon P and Wendt, Toni and Lundqvist, Udda and Mascher, Martin and Stein, Nils and Hansson, Mats},
  issn         = {1573-5028},
  language     = {eng},
  number       = {6},
  pages        = {609--626},
  publisher    = {Kluwer},
  series       = {Plant Molecular Biology},
  title        = {Genetic linkage facilitates cloning of Ert-m regulating plant architecture in barley and identified a strong candidate of Ant1 involved in anthocyanin biosynthesis.},
  url          = {http://dx.doi.org/10.1007/s11103-015-0350-x},
  volume       = {88},
  year         = {2015},
}