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Docetaxel-Loaded Fluorescent Liquid-Crystalline Nanoparticles for Cancer Theranostics.

Meli, Valeria; Caltagirone, Claudia; Falchi, Angela M; Hyde, Stephen T; Lippolis, Vito; Monduzzi, Maura; Obiols-Rabasa, Marc LU ; Rosa, Antonella; Schmidt, Judith and Talmon, Yeshayahu, et al. (2015) In Langmuir 31(35). p.9566-9575
Abstract
Here, we describe a novel monoolein-based cubosome formulation engineered for possible theranostic applications in oncology. The Docetaxel-loaded nanoparticles were stabilized in water by a mixture of commercial Pluronic (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer) F108 (PF108) and rhodamine- and folate-conjugated PF108 so that the nanoparticles possess targeting, therapeutic, and imaging properties. Nanoparticles were investigated by DLS, cryo-TEM, and SAXS to confirm their structural features. The fluorescent emission characterization of the proposed formulation indicated that the rhodamine conjugated to the PF108 experiences an environment less polar than water (similar to chloroform), suggesting... (More)
Here, we describe a novel monoolein-based cubosome formulation engineered for possible theranostic applications in oncology. The Docetaxel-loaded nanoparticles were stabilized in water by a mixture of commercial Pluronic (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer) F108 (PF108) and rhodamine- and folate-conjugated PF108 so that the nanoparticles possess targeting, therapeutic, and imaging properties. Nanoparticles were investigated by DLS, cryo-TEM, and SAXS to confirm their structural features. The fluorescent emission characterization of the proposed formulation indicated that the rhodamine conjugated to the PF108 experiences an environment less polar than water (similar to chloroform), suggesting that the fluorescent fragment is buried within the poly(ethylene oxide) corona surrounding the nanoparticle. Furthermore, these nanoparticles were successfully used to image living HeLa cells and demonstrated a significant short-term (4 h incubation) cytotoxicity effect against these cancer cells. Furthermore, given their analogy as nanocarriers for molecules of pharmaceutical interest and to better stress the singularities of these bicontinuous cubic nanoparticles, we also quantitatively evaluated the differences between cubosomes and multilamellar liposomes in terms of surface area and hydrophobic volume. (Less)
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type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
31
issue
35
pages
9566 - 9575
publisher
The American Chemical Society
external identifiers
  • pmid:26293620
  • wos:000361087000006
  • scopus:84941141692
ISSN
0743-7463
DOI
10.1021/acs.langmuir.5b02101
language
English
LU publication?
yes
id
261c2d6b-2eb6-4c73-8d93-f5f199fb958b (old id 7840423)
date added to LUP
2015-09-13 20:45:31
date last changed
2017-10-08 03:05:27
@article{261c2d6b-2eb6-4c73-8d93-f5f199fb958b,
  abstract     = {Here, we describe a novel monoolein-based cubosome formulation engineered for possible theranostic applications in oncology. The Docetaxel-loaded nanoparticles were stabilized in water by a mixture of commercial Pluronic (poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer) F108 (PF108) and rhodamine- and folate-conjugated PF108 so that the nanoparticles possess targeting, therapeutic, and imaging properties. Nanoparticles were investigated by DLS, cryo-TEM, and SAXS to confirm their structural features. The fluorescent emission characterization of the proposed formulation indicated that the rhodamine conjugated to the PF108 experiences an environment less polar than water (similar to chloroform), suggesting that the fluorescent fragment is buried within the poly(ethylene oxide) corona surrounding the nanoparticle. Furthermore, these nanoparticles were successfully used to image living HeLa cells and demonstrated a significant short-term (4 h incubation) cytotoxicity effect against these cancer cells. Furthermore, given their analogy as nanocarriers for molecules of pharmaceutical interest and to better stress the singularities of these bicontinuous cubic nanoparticles, we also quantitatively evaluated the differences between cubosomes and multilamellar liposomes in terms of surface area and hydrophobic volume.},
  author       = {Meli, Valeria and Caltagirone, Claudia and Falchi, Angela M and Hyde, Stephen T and Lippolis, Vito and Monduzzi, Maura and Obiols-Rabasa, Marc and Rosa, Antonella and Schmidt, Judith and Talmon, Yeshayahu and Murgia, Sergio},
  issn         = {0743-7463},
  language     = {eng},
  number       = {35},
  pages        = {9566--9575},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Docetaxel-Loaded Fluorescent Liquid-Crystalline Nanoparticles for Cancer Theranostics.},
  url          = {http://dx.doi.org/10.1021/acs.langmuir.5b02101},
  volume       = {31},
  year         = {2015},
}