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Preparative isolation of PCR products using mixed-mode chromatography.

Matos, Tiago LU ; Dos Santos Silva, Graciela LU ; Queiroz, J A and Bülow, Leif LU (2015) In Analytical Biochemistry 489(Online 14 August 2015). p.73-75
Abstract
The polymerase chain reaction (PCR) has become one of the most useful techniques in molecular biology laboratories around the world. The purification of the target DNA product is, however, often challenging and most users are restricted to employing available commercial kits. The recent developments in mixed-mode chromatography have shown higher selectivity for a variety of nucleic acids containing samples. Capto Adhere is a mixed-mode chromatography resin that offers a high selectivity ligand and is here applied for the purification of amplified DNAs from PCR mixtures in a 10 minutes' single step, with yields above 95%, high linearity and precision for different concentrations.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Biochemistry
volume
489
issue
Online 14 August 2015
pages
73 - 75
publisher
Elsevier
external identifiers
  • pmid:26283568
  • wos:000362623500012
  • scopus:84942163159
ISSN
1096-0309
DOI
10.1016/j.ab.2015.08.009
language
English
LU publication?
yes
id
bf97b017-ec1d-4b52-b71a-df705128912e (old id 7840710)
date added to LUP
2015-09-13 20:41:50
date last changed
2017-01-01 04:05:30
@article{bf97b017-ec1d-4b52-b71a-df705128912e,
  abstract     = {The polymerase chain reaction (PCR) has become one of the most useful techniques in molecular biology laboratories around the world. The purification of the target DNA product is, however, often challenging and most users are restricted to employing available commercial kits. The recent developments in mixed-mode chromatography have shown higher selectivity for a variety of nucleic acids containing samples. Capto Adhere is a mixed-mode chromatography resin that offers a high selectivity ligand and is here applied for the purification of amplified DNAs from PCR mixtures in a 10 minutes' single step, with yields above 95%, high linearity and precision for different concentrations.},
  author       = {Matos, Tiago and Dos Santos Silva, Graciela and Queiroz, J A and Bülow, Leif},
  issn         = {1096-0309},
  language     = {eng},
  number       = {Online 14 August 2015},
  pages        = {73--75},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {Preparative isolation of PCR products using mixed-mode chromatography.},
  url          = {http://dx.doi.org/10.1016/j.ab.2015.08.009},
  volume       = {489},
  year         = {2015},
}