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Identification of the female-produced sex pheromone of the leafminer Holocacista capensis infesting grapevine in South Africa.

Wang, Hong-Lei LU ; Geertsema, Henk; van Nieukerken, Erik J and Löfstedt, Christer LU (2015) In Journal of Chemical Ecology 41(8). p.724-731
Abstract
We report the first identification of a sex pheromone in a heliozelid moth, Holocacista capensis van Nieukerken & Geertsema. This leafminer recently infested grapevine in South Africa. Compared to solvent extraction of pheromone glands, solid phase microextraction (SPME) proved to be highly effective for collection of the pheromone from calling females. The volatiles collected by SPME were analyzed by gas chromatography with electroantennographic detection (GC/EAD). Three compounds eliciting electrophysiological activity from the male antenna were identified as (Z)-5-tetradecenal, (Z)-7-tetradecenal, and (Z)-9-hexadecenal by coupled gas chromatography-mass spectrometry (GC/MS). GC/MS analysis of dimethyldisulphide (DMDS)... (More)
We report the first identification of a sex pheromone in a heliozelid moth, Holocacista capensis van Nieukerken & Geertsema. This leafminer recently infested grapevine in South Africa. Compared to solvent extraction of pheromone glands, solid phase microextraction (SPME) proved to be highly effective for collection of the pheromone from calling females. The volatiles collected by SPME were analyzed by gas chromatography with electroantennographic detection (GC/EAD). Three compounds eliciting electrophysiological activity from the male antenna were identified as (Z)-5-tetradecenal, (Z)-7-tetradecenal, and (Z)-9-hexadecenal by coupled gas chromatography-mass spectrometry (GC/MS). GC/MS analysis of dimethyldisulphide (DMDS) derivatives of fatty acyl moieties in pheromone gland extracts confirmed the presence of the corresponding putative pheromone precursors with double bonds in the same position and with Z geometry. Field trapping experiments in a South African vineyard confirmed that both (Z)-5-tetradecenal and (Z)-7-tetradecenal are essential for the attraction of male H. capensis, whereas addition of (Z)-9-hexadecenal to the blend did not affect the attractiveness. The composition of the pheromone is discussed in relation to the phylogeny of this family of moths. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chemical Ecology
volume
41
issue
8
pages
724 - 731
publisher
Springer
external identifiers
  • pmid:26271672
  • wos:000361467500005
  • scopus:84941944007
ISSN
1573-1561
DOI
10.1007/s10886-015-0611-9
project
Evolutionary mechanisms of pheromone divergence in Lepidoptera
language
English
LU publication?
yes
id
f5ff43b8-deea-4750-9443-4cf2036d1fc5 (old id 7841173)
date added to LUP
2015-09-10 08:37:55
date last changed
2017-01-01 03:55:52
@article{f5ff43b8-deea-4750-9443-4cf2036d1fc5,
  abstract     = {We report the first identification of a sex pheromone in a heliozelid moth, <i>Holocacista capensis</i> van Nieukerken &amp; Geertsema. This leafminer recently infested grapevine in South Africa. Compared to solvent extraction of pheromone glands, solid phase microextraction (SPME) proved to be highly effective for collection of the pheromone from calling females. The volatiles collected by SPME were analyzed by gas chromatography with electroantennographic detection (GC/EAD). Three compounds eliciting electrophysiological activity from the male antenna were identified as (Z)-5-tetradecenal, (Z)-7-tetradecenal, and (Z)-9-hexadecenal by coupled gas chromatography-mass spectrometry (GC/MS). GC/MS analysis of dimethyldisulphide (DMDS) derivatives of fatty acyl moieties in pheromone gland extracts confirmed the presence of the corresponding putative pheromone precursors with double bonds in the same position and with Z geometry. Field trapping experiments in a South African vineyard confirmed that both (Z)-5-tetradecenal and (Z)-7-tetradecenal are essential for the attraction of male H. capensis, whereas addition of (Z)-9-hexadecenal to the blend did not affect the attractiveness. The composition of the pheromone is discussed in relation to the phylogeny of this family of moths.},
  author       = {Wang, Hong-Lei and Geertsema, Henk and van Nieukerken, Erik J and Löfstedt, Christer},
  issn         = {1573-1561},
  language     = {eng},
  number       = {8},
  pages        = {724--731},
  publisher    = {Springer},
  series       = {Journal of Chemical Ecology},
  title        = {Identification of the female-produced sex pheromone of the leafminer <i>Holocacista capensis</i> infesting grapevine in South Africa.},
  url          = {http://dx.doi.org/10.1007/s10886-015-0611-9},
  volume       = {41},
  year         = {2015},
}