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Support of Neuronal Growth Over Glial Growth and Guidance of Optic Nerve Axons by Vertical Nanowire Arrays.

Offranc-Piret, Gaelle LU ; Perez, Maria Thereza LU and Prinz, Christelle LU (2015) In ACS Applied Materials and Interfaces 7(34). p.18944-18948
Abstract
Neural cultures are very useful in neuroscience, providing simpler and better controlled systems than the in vivo situation. Neural tissue contains two main cell types, neurons and glia, and interactions between these are essential for appropriate neuronal development. In neural cultures, glial cells tend to overgrow neurons, limiting the access to neuronal interrogation. There is therefore a pressing need for improved systems that enable a good separation when coculturing neurons and glial cells simultaneously, allowing one to address the neurons unequivocally. Here, we used substrates consisting of dense arrays of vertical nanowires intercalated by flat regions to separate retinal neurons and glial cells in distinct, but neighboring,... (More)
Neural cultures are very useful in neuroscience, providing simpler and better controlled systems than the in vivo situation. Neural tissue contains two main cell types, neurons and glia, and interactions between these are essential for appropriate neuronal development. In neural cultures, glial cells tend to overgrow neurons, limiting the access to neuronal interrogation. There is therefore a pressing need for improved systems that enable a good separation when coculturing neurons and glial cells simultaneously, allowing one to address the neurons unequivocally. Here, we used substrates consisting of dense arrays of vertical nanowires intercalated by flat regions to separate retinal neurons and glial cells in distinct, but neighboring, compartments. We also generated a nanowire patterning capable of guiding optic nerve axons. The results will facilitate the design of surfaces aimed at studying and controlling neuronal networks. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
ACS Applied Materials and Interfaces
volume
7
issue
34
pages
18944 - 18948
publisher
The American Chemical Society
external identifiers
  • pmid:26262507
  • wos:000360868700009
  • scopus:84941286910
ISSN
1944-8244
DOI
10.1021/acsami.5b03798
language
English
LU publication?
yes
id
3ce5b59f-9cbb-4741-984b-d4638b508864 (old id 7844239)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26262507?dopt=Abstract
date added to LUP
2015-09-05 16:19:07
date last changed
2017-10-31 15:08:20
@article{3ce5b59f-9cbb-4741-984b-d4638b508864,
  abstract     = {Neural cultures are very useful in neuroscience, providing simpler and better controlled systems than the in vivo situation. Neural tissue contains two main cell types, neurons and glia, and interactions between these are essential for appropriate neuronal development. In neural cultures, glial cells tend to overgrow neurons, limiting the access to neuronal interrogation. There is therefore a pressing need for improved systems that enable a good separation when coculturing neurons and glial cells simultaneously, allowing one to address the neurons unequivocally. Here, we used substrates consisting of dense arrays of vertical nanowires intercalated by flat regions to separate retinal neurons and glial cells in distinct, but neighboring, compartments. We also generated a nanowire patterning capable of guiding optic nerve axons. The results will facilitate the design of surfaces aimed at studying and controlling neuronal networks.},
  author       = {Offranc-Piret, Gaelle and Perez, Maria Thereza and Prinz, Christelle},
  issn         = {1944-8244},
  language     = {eng},
  number       = {34},
  pages        = {18944--18948},
  publisher    = {The American Chemical Society},
  series       = {ACS Applied Materials and Interfaces},
  title        = {Support of Neuronal Growth Over Glial Growth and Guidance of Optic Nerve Axons by Vertical Nanowire Arrays.},
  url          = {http://dx.doi.org/10.1021/acsami.5b03798},
  volume       = {7},
  year         = {2015},
}