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Influence of Internal DNA Pressure on Stability and Infectivity of Phage λ.

Bauer, D W and Evilevitch, Alex LU (2015) In Journal of Molecular Biology 427(20). p.3189-3200
Abstract
Viruses must remain infectious while in harsh extracellular environments. An important aspect of viral particle stability for double-stranded DNA viruses is the energetically unfavorable state of the tightly confined DNA chain within the virus capsid creating pressures of tens of atmospheres. Here, we study the influence of internal genome pressure on the thermal stability of viral particles. Using differential scanning calorimetry to monitor genome loss upon heating, we find that internal pressure destabilizes the virion, resulting in a smaller activation energy barrier to trigger DNA release. These experiments are complemented by plaque assay and electron microscopy measurements to determine the influence of intra-capsid DNA pressure on... (More)
Viruses must remain infectious while in harsh extracellular environments. An important aspect of viral particle stability for double-stranded DNA viruses is the energetically unfavorable state of the tightly confined DNA chain within the virus capsid creating pressures of tens of atmospheres. Here, we study the influence of internal genome pressure on the thermal stability of viral particles. Using differential scanning calorimetry to monitor genome loss upon heating, we find that internal pressure destabilizes the virion, resulting in a smaller activation energy barrier to trigger DNA release. These experiments are complemented by plaque assay and electron microscopy measurements to determine the influence of intra-capsid DNA pressure on the rates of viral infectivity loss. At higher temperatures (65-75°C), failure to retain the packaged genome is the dominant mechanism of viral inactivation. Conversely, at lower temperatures (40-55°C), a separate inactivation mechanism dominates, which results in non-infectious particles that still retain their packaged DNA. Most significantly, both mechanisms of infectivity loss are directly influenced by internal DNA pressure, with higher pressure resulting in a more rapid rate of inactivation at all temperatures. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Molecular Biology
volume
427
issue
20
pages
3189 - 3200
publisher
Elsevier
external identifiers
  • pmid:26254570
  • wos:000362611300001
  • scopus:84942368395
ISSN
1089-8638
DOI
10.1016/j.jmb.2015.07.023
language
English
LU publication?
yes
id
f45bd396-c1f7-49af-b83a-a78737456df5 (old id 7844357)
date added to LUP
2015-09-13 20:39:25
date last changed
2017-02-05 03:05:22
@article{f45bd396-c1f7-49af-b83a-a78737456df5,
  abstract     = {Viruses must remain infectious while in harsh extracellular environments. An important aspect of viral particle stability for double-stranded DNA viruses is the energetically unfavorable state of the tightly confined DNA chain within the virus capsid creating pressures of tens of atmospheres. Here, we study the influence of internal genome pressure on the thermal stability of viral particles. Using differential scanning calorimetry to monitor genome loss upon heating, we find that internal pressure destabilizes the virion, resulting in a smaller activation energy barrier to trigger DNA release. These experiments are complemented by plaque assay and electron microscopy measurements to determine the influence of intra-capsid DNA pressure on the rates of viral infectivity loss. At higher temperatures (65-75°C), failure to retain the packaged genome is the dominant mechanism of viral inactivation. Conversely, at lower temperatures (40-55°C), a separate inactivation mechanism dominates, which results in non-infectious particles that still retain their packaged DNA. Most significantly, both mechanisms of infectivity loss are directly influenced by internal DNA pressure, with higher pressure resulting in a more rapid rate of inactivation at all temperatures.},
  author       = {Bauer, D W and Evilevitch, Alex},
  issn         = {1089-8638},
  language     = {eng},
  number       = {20},
  pages        = {3189--3200},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Biology},
  title        = {Influence of Internal DNA Pressure on Stability and Infectivity of Phage λ.},
  url          = {http://dx.doi.org/10.1016/j.jmb.2015.07.023},
  volume       = {427},
  year         = {2015},
}