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Retinal functional alterations in mice lacking intermediate filament proteins glial fibrillary acidic protein and vimentin.

Wunderlich, Kirsten LU ; Tanimoto, Naoyuki; Grosche, Antje; Zrenner, Eberhart; Pekny, Milos; Reichenbach, Andreas; Seeliger, Mathias W; Pannicke, Thomas and Perez, Maria Thereza LU (2015) In FASEB Journal 29(12). p.4815-4828
Abstract
Vimentin (Vim) and glial fibrillary acidic protein (GFAP) are important components of the intermediate filament (IF) (or nanofilament) system of astroglial cells. We conducted full-field electroretinogram (ERG) recordings and found that whereas photoreceptor responses (a-wave) were normal in uninjured GFAP(-/-)Vim(-/-) mice, b-wave amplitudes were increased. Moreover, we found that Kir (inward rectifier K(+)) channel protein expression was reduced in the retinas of GFAP(-/-)Vim(-/-) mice and that Kir-mediated current amplitudes were lower in Müller glial cells isolated from these mice. Studies have shown that the IF system, in addition, is involved in the retinal response to injury and that attenuated Müller cell reactivity and reduced... (More)
Vimentin (Vim) and glial fibrillary acidic protein (GFAP) are important components of the intermediate filament (IF) (or nanofilament) system of astroglial cells. We conducted full-field electroretinogram (ERG) recordings and found that whereas photoreceptor responses (a-wave) were normal in uninjured GFAP(-/-)Vim(-/-) mice, b-wave amplitudes were increased. Moreover, we found that Kir (inward rectifier K(+)) channel protein expression was reduced in the retinas of GFAP(-/-)Vim(-/-) mice and that Kir-mediated current amplitudes were lower in Müller glial cells isolated from these mice. Studies have shown that the IF system, in addition, is involved in the retinal response to injury and that attenuated Müller cell reactivity and reduced photoreceptor cell loss are observed in IF-deficient mice after experimental retinal detachment. We investigated whether the lack of IF proteins would affect cell survival in a retinal ischemia-reperfusion model. We found that although cell loss was induced in both genotypes, the number of surviving cells in the inner retina was lower in IF-deficient mice. Our findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition.-Wunderlich, K. A., Tanimoto, N., Grosche, A., Zrenner E., Pekny, M., Reichenbach, A., Seeliger, M. W., Pannicke, T., Perez, M.-T. Retinal functional alterations in mice lacking intermediate filament proteins glial fibrillary acidic protein and vimentin. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FASEB Journal
volume
29
issue
12
pages
4815 - 4828
publisher
The Federation of American Societies for Experimental Biology
external identifiers
  • pmid:26251181
  • wos:000365523600009
  • scopus:84973437277
ISSN
1530-6860
DOI
10.1096/fj.15-272963
language
English
LU publication?
yes
id
b49843ed-be30-4ee5-aab8-d556dcec3457 (old id 7844462)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26251181?dopt=Abstract
date added to LUP
2015-09-05 15:17:39
date last changed
2017-10-31 15:07:57
@article{b49843ed-be30-4ee5-aab8-d556dcec3457,
  abstract     = {Vimentin (Vim) and glial fibrillary acidic protein (GFAP) are important components of the intermediate filament (IF) (or nanofilament) system of astroglial cells. We conducted full-field electroretinogram (ERG) recordings and found that whereas photoreceptor responses (a-wave) were normal in uninjured GFAP(-/-)Vim(-/-) mice, b-wave amplitudes were increased. Moreover, we found that Kir (inward rectifier K(+)) channel protein expression was reduced in the retinas of GFAP(-/-)Vim(-/-) mice and that Kir-mediated current amplitudes were lower in Müller glial cells isolated from these mice. Studies have shown that the IF system, in addition, is involved in the retinal response to injury and that attenuated Müller cell reactivity and reduced photoreceptor cell loss are observed in IF-deficient mice after experimental retinal detachment. We investigated whether the lack of IF proteins would affect cell survival in a retinal ischemia-reperfusion model. We found that although cell loss was induced in both genotypes, the number of surviving cells in the inner retina was lower in IF-deficient mice. Our findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition.-Wunderlich, K. A., Tanimoto, N., Grosche, A., Zrenner E., Pekny, M., Reichenbach, A., Seeliger, M. W., Pannicke, T., Perez, M.-T. Retinal functional alterations in mice lacking intermediate filament proteins glial fibrillary acidic protein and vimentin.},
  author       = {Wunderlich, Kirsten and Tanimoto, Naoyuki and Grosche, Antje and Zrenner, Eberhart and Pekny, Milos and Reichenbach, Andreas and Seeliger, Mathias W and Pannicke, Thomas and Perez, Maria Thereza},
  issn         = {1530-6860},
  language     = {eng},
  number       = {12},
  pages        = {4815--4828},
  publisher    = {The Federation of American Societies for Experimental Biology},
  series       = {FASEB Journal},
  title        = {Retinal functional alterations in mice lacking intermediate filament proteins glial fibrillary acidic protein and vimentin.},
  url          = {http://dx.doi.org/10.1096/fj.15-272963},
  volume       = {29},
  year         = {2015},
}