A peroxisomally localized acyl-activating enzyme is required for volatile benzenoid formation in a Petuniaxhybrida cv. 'Mitchell Diploid' flower
(2012) In Journal of Experimental Botany 63(13). p.33-4821- Abstract
Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, β-oxidative scheme. Metabolic flux analysis, reverse genetics, and biochemical characterizations of key enzymes in this pathway have supported this putative concept. However, the theoretical first enzymatic reaction, which leads to the production of cinnamoyl-CoA, has only been physically demonstrated in a select number of bacteria like Streptomyces maritimus through mutagenesis and recombinant... (More)
Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, β-oxidative scheme. Metabolic flux analysis, reverse genetics, and biochemical characterizations of key enzymes in this pathway have supported this putative concept. However, the theoretical first enzymatic reaction, which leads to the production of cinnamoyl-CoA, has only been physically demonstrated in a select number of bacteria like Streptomyces maritimus through mutagenesis and recombinant protein production. A transcript has been cloned and characterized from MD flowers that shares high homology with an Arabidopsis thaliana transcript ACYL-ACTIVATING ENZYME11 (AtAAE11) and the S. maritimus ACYL-COA:LIGASE (SmEncH). In MD, the PhAAE transcript accumulates in a very similar manner as bona fide FVBP network genes, i.e. high levels in an open flower petal and ethylene regulated. In planta, PhAAE is localized to the peroxisome. Upon reduction of PhAAE transcript through a stable RNAi approach, transgenic flowers emitted a reduced level of all benzenoid volatile compounds. Together, the data suggest that PhAAE may be responsible for the activation of t-cinnamic acid, which would be required for floral volatile benzenoid production in MD.
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- author
- Colquhoun, Thomas A ; Marciniak, Danielle M ; Wedde, Ashlyn E ; Kim, Joo Young ; Schwieterman, Michael L ; Levin, Laura A ; Van Moerkercke, Alex LU ; Schuurink, Robert C and Clark, David G
- publishing date
- 2012-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amino Acid Sequence, Benzene Derivatives, DNA, Plant, Flowers, Gene Expression Regulation, Plant, Metabolic Networks and Pathways, Microscopy, Confocal, Molecular Sequence Data, Peroxisomes, Petunia, Phylogeny, Plant Proteins, Plant Roots, Plant Stems, Propanols, RNA Interference, RNA, Messenger, RNA, Plant, Recombinant Proteins, Sequence Alignment, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
- in
- Journal of Experimental Botany
- volume
- 63
- issue
- 13
- pages
- 13 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:84865454034
- pmid:22771854
- ISSN
- 0022-0957
- DOI
- 10.1093/jxb/ers153
- language
- English
- LU publication?
- no
- id
- 784c2a0b-2737-4fbc-b389-1b400f44a4eb
- date added to LUP
- 2017-11-06 11:31:17
- date last changed
- 2024-03-01 01:28:36
@article{784c2a0b-2737-4fbc-b389-1b400f44a4eb,
abstract = {{<p>Floral volatile benzenoid/phenylpropanoid (FVBP) biosynthesis is a complex and coordinate cellular process executed by petal limb cells of a Petunia×hybrida cv. 'Mitchell Diploid' (MD) plant. In MD flowers, the majority of benzenoid volatile compounds are derived from a core phenylpropanoid pathway intermediate by a coenzyme A (CoA) dependent, β-oxidative scheme. Metabolic flux analysis, reverse genetics, and biochemical characterizations of key enzymes in this pathway have supported this putative concept. However, the theoretical first enzymatic reaction, which leads to the production of cinnamoyl-CoA, has only been physically demonstrated in a select number of bacteria like Streptomyces maritimus through mutagenesis and recombinant protein production. A transcript has been cloned and characterized from MD flowers that shares high homology with an Arabidopsis thaliana transcript ACYL-ACTIVATING ENZYME11 (AtAAE11) and the S. maritimus ACYL-COA:LIGASE (SmEncH). In MD, the PhAAE transcript accumulates in a very similar manner as bona fide FVBP network genes, i.e. high levels in an open flower petal and ethylene regulated. In planta, PhAAE is localized to the peroxisome. Upon reduction of PhAAE transcript through a stable RNAi approach, transgenic flowers emitted a reduced level of all benzenoid volatile compounds. Together, the data suggest that PhAAE may be responsible for the activation of t-cinnamic acid, which would be required for floral volatile benzenoid production in MD.</p>}},
author = {{Colquhoun, Thomas A and Marciniak, Danielle M and Wedde, Ashlyn E and Kim, Joo Young and Schwieterman, Michael L and Levin, Laura A and Van Moerkercke, Alex and Schuurink, Robert C and Clark, David G}},
issn = {{0022-0957}},
keywords = {{Amino Acid Sequence; Benzene Derivatives; DNA, Plant; Flowers; Gene Expression Regulation, Plant; Metabolic Networks and Pathways; Microscopy, Confocal; Molecular Sequence Data; Peroxisomes; Petunia; Phylogeny; Plant Proteins; Plant Roots; Plant Stems; Propanols; RNA Interference; RNA, Messenger; RNA, Plant; Recombinant Proteins; Sequence Alignment; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.}},
language = {{eng}},
number = {{13}},
pages = {{33--4821}},
publisher = {{Oxford University Press}},
series = {{Journal of Experimental Botany}},
title = {{A peroxisomally localized acyl-activating enzyme is required for volatile benzenoid formation in a Petuniaxhybrida cv. 'Mitchell Diploid' flower}},
url = {{http://dx.doi.org/10.1093/jxb/ers153}},
doi = {{10.1093/jxb/ers153}},
volume = {{63}},
year = {{2012}},
}