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Measurement of urinary S100B protein concentrations for the early identification of brain damage in asphyxiated full-term infants

Gazzolo, D; Marinoni, E; Di Iorio, R; Bruschettini, Matteo LU ; Kornacka, M; Lituania, M; Majewska, U; Serra, G and Michetti, F (2003) In Archives of Pediatrics & Adolescent Medicine 157(12). p.1163-1168
Abstract
Background: Perinatal asphyxia is a major cause of mortality and morbidity. To date there are no reliable methods to detect which infants will develop brain damage after asphyxia insult. We investigated whether measurements of urine levels of S100B in asphyxiated full-term newborns maybe a useful tool for early detection of post-asphyxia brain damage. Methods: A prospective study of 38 infants with perinatal asphyxia and 96 control subjects, recruited at 3 tertiary departments of neonatology between April 1, 1999, and July 31, 2001. Routine laboratory variables, neurologic patterns, and urine concentrations of S100B protein were determined at 4 predetermined time points (first urination and 12, 24, and 72 hours after birth). The... (More)
Background: Perinatal asphyxia is a major cause of mortality and morbidity. To date there are no reliable methods to detect which infants will develop brain damage after asphyxia insult. We investigated whether measurements of urine levels of S100B in asphyxiated full-term newborns maybe a useful tool for early detection of post-asphyxia brain damage. Methods: A prospective study of 38 infants with perinatal asphyxia and 96 control subjects, recruited at 3 tertiary departments of neonatology between April 1, 1999, and July 31, 2001. Routine laboratory variables, neurologic patterns, and urine concentrations of S100B protein were determined at 4 predetermined time points (first urination and 12, 24, and 72 hours after birth). The concentrations of S100B protein in urine were measured using an immunoluminometric assay. The results were correlated with the presence or absence of neurologic abnormalities at age 12 months. Results: S100B protein levels were significantly higher in samples collected at all monitoring times from newhorns with abnormal neurologic findings on follow-up (first urination, 1.92 +/- 0.33 mug/L; 12 hours, 2.78 +/- 1.71 mug/L; 24 hours, 4.75 +/- 4.08 mug/L; 72 hours, 5.93 +/- 1.0 pg/L) than in samples from those without (first urination,0.24 +/- 0.06 mug/L; 12 hours, 0.13 +/- 0.06 mug/L; 24 hours, 0.21 +/- 0.07 mug/L; 72 hours, 0.12 +/- 0.04 mug/L) or from healthy infants (first urination, 0.11 +/- 0.01 mug/L; 12 hours, 0.12 +/- 0.03 mug/L; 24 hours, 0.12 +/- 0.02 mug/L; 72 hours, 0.12 +/- 0.02 mug/L) (P < .001 forall). An S100B concentration cutoff of 0.28 pg/L at first urination had a sensitivity of 100% and a specificity of 87.3% for predicting the development of abnormal neurologic findings on followup. The sensitivity and specificity of measurements obtained between 12 and 72 hours were up to 100% and 98.2%, respectively. Conclusion: Longitudinal S100B protein measurements in urine soon after birth are a useful tool to identify which asphyxiated infants are at risk of long-term neurologic sequelae. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Archives of Pediatrics & Adolescent Medicine
volume
157
issue
12
pages
1163 - 1168
publisher
American Medical Association
external identifiers
  • wos:000186959800006
  • scopus:0345655297
ISSN
1072-4710
DOI
10.1001/archpedi.157.12.1163
language
English
LU publication?
no
id
d2e6da88-fb05-4263-affc-3b8fabdc0786 (old id 7856382)
date added to LUP
2015-09-07 10:50:18
date last changed
2018-10-14 04:13:58
@article{d2e6da88-fb05-4263-affc-3b8fabdc0786,
  abstract     = {Background: Perinatal asphyxia is a major cause of mortality and morbidity. To date there are no reliable methods to detect which infants will develop brain damage after asphyxia insult. We investigated whether measurements of urine levels of S100B in asphyxiated full-term newborns maybe a useful tool for early detection of post-asphyxia brain damage. Methods: A prospective study of 38 infants with perinatal asphyxia and 96 control subjects, recruited at 3 tertiary departments of neonatology between April 1, 1999, and July 31, 2001. Routine laboratory variables, neurologic patterns, and urine concentrations of S100B protein were determined at 4 predetermined time points (first urination and 12, 24, and 72 hours after birth). The concentrations of S100B protein in urine were measured using an immunoluminometric assay. The results were correlated with the presence or absence of neurologic abnormalities at age 12 months. Results: S100B protein levels were significantly higher in samples collected at all monitoring times from newhorns with abnormal neurologic findings on follow-up (first urination, 1.92 +/- 0.33 mug/L; 12 hours, 2.78 +/- 1.71 mug/L; 24 hours, 4.75 +/- 4.08 mug/L; 72 hours, 5.93 +/- 1.0 pg/L) than in samples from those without (first urination,0.24 +/- 0.06 mug/L; 12 hours, 0.13 +/- 0.06 mug/L; 24 hours, 0.21 +/- 0.07 mug/L; 72 hours, 0.12 +/- 0.04 mug/L) or from healthy infants (first urination, 0.11 +/- 0.01 mug/L; 12 hours, 0.12 +/- 0.03 mug/L; 24 hours, 0.12 +/- 0.02 mug/L; 72 hours, 0.12 +/- 0.02 mug/L) (P &lt; .001 forall). An S100B concentration cutoff of 0.28 pg/L at first urination had a sensitivity of 100% and a specificity of 87.3% for predicting the development of abnormal neurologic findings on followup. The sensitivity and specificity of measurements obtained between 12 and 72 hours were up to 100% and 98.2%, respectively. Conclusion: Longitudinal S100B protein measurements in urine soon after birth are a useful tool to identify which asphyxiated infants are at risk of long-term neurologic sequelae.},
  author       = {Gazzolo, D and Marinoni, E and Di Iorio, R and Bruschettini, Matteo and Kornacka, M and Lituania, M and Majewska, U and Serra, G and Michetti, F},
  issn         = {1072-4710},
  language     = {eng},
  number       = {12},
  pages        = {1163--1168},
  publisher    = {American Medical Association},
  series       = {Archives of Pediatrics & Adolescent Medicine},
  title        = {Measurement of urinary S100B protein concentrations for the early identification of brain damage in asphyxiated full-term infants},
  url          = {http://dx.doi.org/10.1001/archpedi.157.12.1163},
  volume       = {157},
  year         = {2003},
}