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Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein

BUCCIARELLI, SASKIA LU ; Mahmoudi, Najet LU ; Casal-Dujat, Lucia LU ; Jehannin, Marie; Jud, Corinne and Stradner, Anna LU (2016) In The Journal of Physical Chemistry Letters 7(9). p.1610-1615
Abstract
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately... (More)
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Letters
volume
7
issue
9
pages
6 pages
publisher
The American Chemical Society
external identifiers
  • scopus:84968754943
  • wos:000375638500002
ISSN
1948-7185
DOI
10.1021/acs.jpclett.6b00593
language
English
LU publication?
yes
id
78a0b6ef-5aab-4e82-856f-e76cf024346b
alternative location
http://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.6b00593
date added to LUP
2016-10-25 16:13:03
date last changed
2017-08-27 06:26:48
@article{78a0b6ef-5aab-4e82-856f-e76cf024346b,
  abstract     = {Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms.},
  author       = {BUCCIARELLI, SASKIA and Mahmoudi, Najet and Casal-Dujat, Lucia and Jehannin, Marie and Jud, Corinne and Stradner, Anna},
  issn         = {1948-7185},
  language     = {eng},
  month        = {05},
  number       = {9},
  pages        = {1610--1615},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Letters},
  title        = {Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein},
  url          = {http://dx.doi.org/10.1021/acs.jpclett.6b00593},
  volume       = {7},
  year         = {2016},
}