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Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein

BUCCIARELLI, SASKIA LU ; Mahmoudi, Najet LU ; Casal-Dujat, Lucia LU ; Jehannin, Marie ; Jud, Corinne and Stradner, Anna LU (2016) In The Journal of Physical Chemistry Letters 7(9). p.1610-1615
Abstract
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately... (More)
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Letters
volume
7
issue
9
pages
6 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:27077243
  • scopus:84968754943
  • wos:000375638500002
ISSN
1948-7185
DOI
10.1021/acs.jpclett.6b00593
language
English
LU publication?
yes
id
78a0b6ef-5aab-4e82-856f-e76cf024346b
alternative location
http://pubs.acs.org/doi/pdf/10.1021/acs.jpclett.6b00593
date added to LUP
2016-10-25 16:13:03
date last changed
2022-04-24 18:31:55
@article{78a0b6ef-5aab-4e82-856f-e76cf024346b,
  abstract     = {{Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D2O for H2O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein gamma B-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature T-c can be described by the extended law of corresponding states for all H2O/D2O ratios, where scaling of the temperature by T-c or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H2O/D2O substitution on gamma B-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms.}},
  author       = {{BUCCIARELLI, SASKIA and Mahmoudi, Najet and Casal-Dujat, Lucia and Jehannin, Marie and Jud, Corinne and Stradner, Anna}},
  issn         = {{1948-7185}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{9}},
  pages        = {{1610--1615}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{The Journal of Physical Chemistry Letters}},
  title        = {{Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein}},
  url          = {{http://dx.doi.org/10.1021/acs.jpclett.6b00593}},
  doi          = {{10.1021/acs.jpclett.6b00593}},
  volume       = {{7}},
  year         = {{2016}},
}