TFEB-mediated autophagy rescues midbrain dopamine neurons from α-synuclein toxicity.
Decressac, Mickael LU ; Mattsson, Bengt LU ; Weikop, Pia ; Lundblad, Martin LU ; Jakobsson, Johan LU
and Björklund, Anders
LU
(2013)
In Proceedings of the National Academy of Sciences
110(19).
p.1817-1826
- Abstract
- The aggregation of α-synuclein plays a major role in Parkinson disease (PD) pathogenesis. Recent evidence suggests that defects in the autophagy-mediated clearance of α-synuclein contribute to the progressive loss of nigral dopamine neurons. Using an in vivo model of α-synuclein toxicity, we show that the PD-like neurodegenerative changes induced by excess cellular levels of α-synuclein in nigral dopamine neurons are closely linked to a progressive decline in markers of lysosome function, accompanied by cytoplasmic retention of transcription factor EB (TFEB), a major transcriptional regulator of the autophagy-lysosome pathway. The changes in lysosomal function, observed in the rat model as well as in human PD midbrain, were reversed by... (More)
- The aggregation of α-synuclein plays a major role in Parkinson disease (PD) pathogenesis. Recent evidence suggests that defects in the autophagy-mediated clearance of α-synuclein contribute to the progressive loss of nigral dopamine neurons. Using an in vivo model of α-synuclein toxicity, we show that the PD-like neurodegenerative changes induced by excess cellular levels of α-synuclein in nigral dopamine neurons are closely linked to a progressive decline in markers of lysosome function, accompanied by cytoplasmic retention of transcription factor EB (TFEB), a major transcriptional regulator of the autophagy-lysosome pathway. The changes in lysosomal function, observed in the rat model as well as in human PD midbrain, were reversed by overexpression of TFEB, which afforded robust neuroprotection via the clearance of α-synuclein oligomers, and were aggravated by microRNA-128-mediated repression of TFEB in both A9 and A10 dopamine neurons. Delayed activation of TFEB function through inhibition of mammalian target of rapamycin blocked α-synuclein induced neurodegeneration and further disease progression. The results provide a mechanistic link between α-synuclein toxicity and impaired TFEB function, and highlight TFEB as a key player in the induction of α-synuclein-induced toxicity and PD pathogenesis, thus identifying TFEB as a promising target for therapies aimed at neuroprotection and disease modification in PD. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3733456
- author
- Decressac, Mickael
LU
; Mattsson, Bengt
LU
; Weikop, Pia
; Lundblad, Martin
LU
; Jakobsson, Johan
LU
and Björklund, Anders
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Proceedings of the National Academy of Sciences
- volume
- 110
- issue
- 19
- pages
- 1817 - 1826
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000319327700015
- pmid:23610405
- scopus:84877351078
- pmid:23610405
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.1305623110
- language
- English
- LU publication?
- yes
- id
- 78bfa4db-5b5f-4b2a-872b-4fdcd3521b43 (old id 3733456)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23610405?dopt=Abstract
- date added to LUP
- 2016-04-01 10:09:06
- date last changed
- 2022-05-17 20:13:51
@article{78bfa4db-5b5f-4b2a-872b-4fdcd3521b43,
abstract = {{The aggregation of α-synuclein plays a major role in Parkinson disease (PD) pathogenesis. Recent evidence suggests that defects in the autophagy-mediated clearance of α-synuclein contribute to the progressive loss of nigral dopamine neurons. Using an in vivo model of α-synuclein toxicity, we show that the PD-like neurodegenerative changes induced by excess cellular levels of α-synuclein in nigral dopamine neurons are closely linked to a progressive decline in markers of lysosome function, accompanied by cytoplasmic retention of transcription factor EB (TFEB), a major transcriptional regulator of the autophagy-lysosome pathway. The changes in lysosomal function, observed in the rat model as well as in human PD midbrain, were reversed by overexpression of TFEB, which afforded robust neuroprotection via the clearance of α-synuclein oligomers, and were aggravated by microRNA-128-mediated repression of TFEB in both A9 and A10 dopamine neurons. Delayed activation of TFEB function through inhibition of mammalian target of rapamycin blocked α-synuclein induced neurodegeneration and further disease progression. The results provide a mechanistic link between α-synuclein toxicity and impaired TFEB function, and highlight TFEB as a key player in the induction of α-synuclein-induced toxicity and PD pathogenesis, thus identifying TFEB as a promising target for therapies aimed at neuroprotection and disease modification in PD.}},
author = {{Decressac, Mickael and Mattsson, Bengt and Weikop, Pia and Lundblad, Martin and Jakobsson, Johan and Björklund, Anders}},
issn = {{1091-6490}},
language = {{eng}},
number = {{19}},
pages = {{1817--1826}},
publisher = {{National Academy of Sciences}},
series = {{Proceedings of the National Academy of Sciences}},
title = {{TFEB-mediated autophagy rescues midbrain dopamine neurons from α-synuclein toxicity.}},
url = {{http://dx.doi.org/10.1073/pnas.1305623110}},
doi = {{10.1073/pnas.1305623110}},
volume = {{110}},
year = {{2013}},
}