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Cadmium, mercury, and lead in kidney cortex are not associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in living kidney donors.

Hossain, Mohammad Bakhtiar ; Barregard, Lars ; Sallsten, Gerd and Broberg Palmgren, Karin LU orcid (2014) In International Archives of Occupational and Environmental Health 87(3). p.315-322
Abstract
PURPOSE: Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not. METHODS: 8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS. RESULTS: The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93... (More)
PURPOSE: Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not. METHODS: 8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS. RESULTS: The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93 and 0.86 nmol/h. In multivariable linear regression analyses, we did not find any association between 8-oxodG concentrations or rates and elements in the kidney. The 24-h 8-oxodG concentrations were positively associated with serum ferritin (β = 0.048, p < 0.0001), body weight (β = 0.13, p = 0.0019), and inversely with gender (β = -3.34, p = 0.0024). Similar associations with 8-oxodG excretion rates were stronger. Smoking was positively associated with 24-h 8-oxodG excretion rates (β = 0.26, p = 0.0090), but not with overnight samples. CONCLUSIONS: Neither cadmium, nor mercury or lead in the kidney contributed to urinary 8-oxodG concentrations in non-occupationally exposed subjects. The iron status was positively associated with urinary 8-oxodG, particularly in women. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Archives of Occupational and Environmental Health
volume
87
issue
3
pages
315 - 322
publisher
Springer
external identifiers
  • pmid:23536210
  • wos:000332953300010
  • scopus:84899407648
  • pmid:23536210
ISSN
1432-1246
DOI
10.1007/s00420-013-0863-z
language
English
LU publication?
yes
id
78cdc1cf-8bf3-4cc1-b587-4f8ced014667 (old id 3627595)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23536210?dopt=Abstract
date added to LUP
2016-04-01 10:52:31
date last changed
2022-04-04 22:08:24
@article{78cdc1cf-8bf3-4cc1-b587-4f8ced014667,
  abstract     = {{PURPOSE: Cadmium in urine is positively associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) concentrations, a sensitive marker of oxidative DNA damage. We determined whether kidney concentrations of cadmium, mercury, and lead, which may generate oxidative DNA damage, were associated with urinary 8-oxodG or not. METHODS: 8-OxodG was measured in separate 24 h and overnight urine samples from Swedish healthy adult kidney donors (N = 152) using LC-MS/MS. Concentrations of metals were measured in kidney biopsies (N = 109) by ICP-MS. RESULTS: The median 8-oxodG concentrations (adjusted to specific gravity) in 24 h and overnight samples were 13.5 and 15.3 nmol/L; 8-oxodG excretion rates in 24 h and overnight samples were 0.93 and 0.86 nmol/h. In multivariable linear regression analyses, we did not find any association between 8-oxodG concentrations or rates and elements in the kidney. The 24-h 8-oxodG concentrations were positively associated with serum ferritin (β = 0.048, p &lt; 0.0001), body weight (β = 0.13, p = 0.0019), and inversely with gender (β = -3.34, p = 0.0024). Similar associations with 8-oxodG excretion rates were stronger. Smoking was positively associated with 24-h 8-oxodG excretion rates (β = 0.26, p = 0.0090), but not with overnight samples. CONCLUSIONS: Neither cadmium, nor mercury or lead in the kidney contributed to urinary 8-oxodG concentrations in non-occupationally exposed subjects. The iron status was positively associated with urinary 8-oxodG, particularly in women.}},
  author       = {{Hossain, Mohammad Bakhtiar and Barregard, Lars and Sallsten, Gerd and Broberg Palmgren, Karin}},
  issn         = {{1432-1246}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{315--322}},
  publisher    = {{Springer}},
  series       = {{International Archives of Occupational and Environmental Health}},
  title        = {{Cadmium, mercury, and lead in kidney cortex are not associated with urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in living kidney donors.}},
  url          = {{http://dx.doi.org/10.1007/s00420-013-0863-z}},
  doi          = {{10.1007/s00420-013-0863-z}},
  volume       = {{87}},
  year         = {{2014}},
}