Isolation of nucleic acids using a polycationic polymer as precipitation agent
(2005) US20050222404A1- Abstract
- The present invention relates to a method of isolating a desired nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+]/[−] between polycationic precipitating agent and nucleic acid is ≧0.5, preferably ≧0.9 and most preferably ≧1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the... (More)
- The present invention relates to a method of isolating a desired nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+]/[−] between polycationic precipitating agent and nucleic acid is ≧0.5, preferably ≧0.9 and most preferably ≧1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the nucleic acid/polycation complex. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/78df0672-314d-4e6c-81d2-017c30ce2c89
- inventor
- Galaev, Igor LU ; Gustavsson, Per-Erik LU ; Izumrudov, Vladimir LU ; Larsson, Per-Olof LU and Wahlund, Per-Olof LU
- organization
- publishing date
- 2005
- type
- Patent
- publication status
- published
- subject
- publisher
- United States Patent Application Publication
- patent number
- US20050222404A1
- language
- English
- LU publication?
- yes
- id
- 78df0672-314d-4e6c-81d2-017c30ce2c89
- date added to LUP
- 2024-06-24 13:01:18
- date last changed
- 2025-04-04 15:04:58
@misc{78df0672-314d-4e6c-81d2-017c30ce2c89,
abstract = {{The present invention relates to a method of isolating a desired nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+]/[−] between polycationic precipitating agent and nucleic acid is ≧0.5, preferably ≧0.9 and most preferably ≧1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the nucleic acid/polycation complex.}},
author = {{Galaev, Igor and Gustavsson, Per-Erik and Izumrudov, Vladimir and Larsson, Per-Olof and Wahlund, Per-Olof}},
language = {{eng}},
note = {{Patent, Patent Number: US20050222404A1}},
publisher = {{United States Patent Application Publication}},
title = {{Isolation of nucleic acids using a polycationic polymer as precipitation agent}},
year = {{2005}},
}