CDK2 is dispensable for adult hippocampal neurogenesis
Vandenbosch, Renaud ; Borgs, Laurence ; Beukelaers, Pierre ; Foidart, Agnès ; Nguyen, Laurent ; Moonen, Gustave ; Berthet, Cyril ; Kaldis, Philipp LU
; Gallo, Vittorio
and Belachew, Shibeshih
, et al.
(2007)
In Cell Cycle
6(24).
p.3065-3069
- Abstract
Granule neurons of the dentate gyrus (DG) of the hippocampus undergo continuous renewal throughout life. Among cell cycle regulators, cyclin-dependent kinase 2 (Cdk2) is considered as a major regulator of S-phase entry. We used Cdk2-deficient mice to decipher the requirement of Cdk2 for the generation of new neurons in the adult hippocampus. The quantification of cell cycle markers first revealed that the lack of Cdk2 activity does not influence spontaneous or seizure-induced proliferation of neural progenitor cells (NPC) in the adult DG. Using bromodeoxyuridine incorporation assays, we showed that the number of mature newborn granule neurons generated de novo was similar in both wild-type (WT) and Cdk2-deficient adult mice. Moreover,... (More)
Granule neurons of the dentate gyrus (DG) of the hippocampus undergo continuous renewal throughout life. Among cell cycle regulators, cyclin-dependent kinase 2 (Cdk2) is considered as a major regulator of S-phase entry. We used Cdk2-deficient mice to decipher the requirement of Cdk2 for the generation of new neurons in the adult hippocampus. The quantification of cell cycle markers first revealed that the lack of Cdk2 activity does not influence spontaneous or seizure-induced proliferation of neural progenitor cells (NPC) in the adult DG. Using bromodeoxyuridine incorporation assays, we showed that the number of mature newborn granule neurons generated de novo was similar in both wild-type (WT) and Cdk2-deficient adult mice. Moreover, the apparent lack of cell output reduction in Cdk2-/- mice DG did not result from a reduction in apoptosis of newborn granule cells as analyzed by TUNEL assays. Our results therefore suggest that Cdk2 is dispensable for NPC proliferation, differentiation and survival of adult-born DG granule neurons in vivo. These data emphasize that functional redundancies between Cdks also occur in the adult brain at the level of neural progenitor cell cycle regulation during hippocampal neurogenesis.
(Less)
- author
- Vandenbosch, Renaud
; Borgs, Laurence
; Beukelaers, Pierre
; Foidart, Agnès
; Nguyen, Laurent
; Moonen, Gustave
; Berthet, Cyril
; Kaldis, Philipp
LU
; Gallo, Vittorio
and Belachew, Shibeshih
, et al. (More)
- Vandenbosch, Renaud
; Borgs, Laurence
; Beukelaers, Pierre
; Foidart, Agnès
; Nguyen, Laurent
; Moonen, Gustave
; Berthet, Cyril
; Kaldis, Philipp
LU
; Gallo, Vittorio
; Belachew, Shibeshih
and Malgrange, Brigitte
(Less)
- publishing date
- 2007-12-15
- type
- Contribution to journal
- publication status
- published
- keywords
- Adult neurogenesis, Apoptosis, Cell cycle, Hippocampus, Seizure
- in
- Cell Cycle
- volume
- 6
- issue
- 24
- pages
- 3065 - 3069
- publisher
- Landes Bioscience
- external identifiers
-
- scopus:38149010217
- pmid:18073538
- ISSN
- 1538-4101
- DOI
- 10.4161/cc.6.24.5048
- language
- English
- LU publication?
- no
- id
- 78e4e1cc-4d22-46af-848c-02adf7a2eb3d
- date added to LUP
- 2019-09-18 14:14:54
- date last changed
- 2024-01-01 20:38:19
@article{78e4e1cc-4d22-46af-848c-02adf7a2eb3d,
abstract = {{<p>Granule neurons of the dentate gyrus (DG) of the hippocampus undergo continuous renewal throughout life. Among cell cycle regulators, cyclin-dependent kinase 2 (Cdk2) is considered as a major regulator of S-phase entry. We used Cdk2-deficient mice to decipher the requirement of Cdk2 for the generation of new neurons in the adult hippocampus. The quantification of cell cycle markers first revealed that the lack of Cdk2 activity does not influence spontaneous or seizure-induced proliferation of neural progenitor cells (NPC) in the adult DG. Using bromodeoxyuridine incorporation assays, we showed that the number of mature newborn granule neurons generated de novo was similar in both wild-type (WT) and Cdk2-deficient adult mice. Moreover, the apparent lack of cell output reduction in Cdk2<sup>-/-</sup> mice DG did not result from a reduction in apoptosis of newborn granule cells as analyzed by TUNEL assays. Our results therefore suggest that Cdk2 is dispensable for NPC proliferation, differentiation and survival of adult-born DG granule neurons in vivo. These data emphasize that functional redundancies between Cdks also occur in the adult brain at the level of neural progenitor cell cycle regulation during hippocampal neurogenesis.</p>}},
author = {{Vandenbosch, Renaud and Borgs, Laurence and Beukelaers, Pierre and Foidart, Agnès and Nguyen, Laurent and Moonen, Gustave and Berthet, Cyril and Kaldis, Philipp and Gallo, Vittorio and Belachew, Shibeshih and Malgrange, Brigitte}},
issn = {{1538-4101}},
keywords = {{Adult neurogenesis; Apoptosis; Cell cycle; Hippocampus; Seizure}},
language = {{eng}},
month = {{12}},
number = {{24}},
pages = {{3065--3069}},
publisher = {{Landes Bioscience}},
series = {{Cell Cycle}},
title = {{CDK2 is dispensable for adult hippocampal neurogenesis}},
url = {{http://dx.doi.org/10.4161/cc.6.24.5048}},
doi = {{10.4161/cc.6.24.5048}},
volume = {{6}},
year = {{2007}},
}