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Effects of various neuropeptide Y/peptide YY fragments on electrically-evoked contractions of the rat vas deferens

Grundemar, L LU and Håkanson, R LU (1990) In British Journal of Pharmacology 100(1). p.190-192
Abstract

1. The effects of various neuropeptide Y (NPY) and peptide YY (PYY) fragments on electrically-evoked twitches in the rat isolated vas deferens were studied and compared with the effects of full length NPY and PYY. The aim was to identify the shortest NPY/PYY fragments that are capable of suppressing the contractions. 2. NPY (1-36) and C-terminal fragments of NPY (from 11-36 to 22-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. On the whole there seemed to be a gradual lowering of the pIC50 values with progressive shortening of the NPY fragments (except for fragments 16-36 and 22-36 that had rather high pIC50 values). NPY 23-36, 24-36 and 25-36 suppressed the twitches at high concentrations (3... (More)

1. The effects of various neuropeptide Y (NPY) and peptide YY (PYY) fragments on electrically-evoked twitches in the rat isolated vas deferens were studied and compared with the effects of full length NPY and PYY. The aim was to identify the shortest NPY/PYY fragments that are capable of suppressing the contractions. 2. NPY (1-36) and C-terminal fragments of NPY (from 11-36 to 22-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. On the whole there seemed to be a gradual lowering of the pIC50 values with progressive shortening of the NPY fragments (except for fragments 16-36 and 22-36 that had rather high pIC50 values). NPY 23-36, 24-36 and 25-36 suppressed the twitches at high concentrations (3 microM). NPY 26-36 was without effect as were C-terminal carboxy-deaminated NPY and glycine extended NPY (NPY-Gly-Lys-Arg). 3. PYY (1-36) and C-terminal fragments of PYY (from 11-36 to 23-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. PYY 1-36 was more potent than any of the fragments. There was a tendency for shorter fragments to have lower pIC50 values. PYY 24-36 and 25-36 suppressed the twitches at high concentrations (3 microM). PYY 26-36 was without effect. 4. The findings suggest that the 12 C-terminal amino acid residues of NPY and PYY are the minimum length required to activate the Y2-receptor.

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keywords
Amino Acid Sequence, Animals, Electric Stimulation, In Vitro Techniques, Male, Molecular Sequence Data, Muscle Contraction/drug effects, Muscle, Smooth/drug effects, Neuropeptide Y/pharmacology, Peptide Fragments/pharmacology, Peptide YY, Peptides/pharmacology, Rats, Rats, Inbred Strains, Swine, Vas Deferens/drug effects
in
British Journal of Pharmacology
volume
100
issue
1
pages
190 - 192
publisher
The British Pharmacological Society
external identifiers
  • pmid:2372659
  • scopus:0025317573
ISSN
0007-1188
DOI
10.1111/j.1476-5381.1990.tb12075.x
language
English
LU publication?
yes
id
78e9e6ee-039f-4287-985a-98a7ed2ee5b0
date added to LUP
2019-09-03 14:12:25
date last changed
2020-01-13 02:19:31
@article{78e9e6ee-039f-4287-985a-98a7ed2ee5b0,
  abstract     = {<p>1. The effects of various neuropeptide Y (NPY) and peptide YY (PYY) fragments on electrically-evoked twitches in the rat isolated vas deferens were studied and compared with the effects of full length NPY and PYY. The aim was to identify the shortest NPY/PYY fragments that are capable of suppressing the contractions. 2. NPY (1-36) and C-terminal fragments of NPY (from 11-36 to 22-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. On the whole there seemed to be a gradual lowering of the pIC50 values with progressive shortening of the NPY fragments (except for fragments 16-36 and 22-36 that had rather high pIC50 values). NPY 23-36, 24-36 and 25-36 suppressed the twitches at high concentrations (3 microM). NPY 26-36 was without effect as were C-terminal carboxy-deaminated NPY and glycine extended NPY (NPY-Gly-Lys-Arg). 3. PYY (1-36) and C-terminal fragments of PYY (from 11-36 to 23-36) suppressed the electrically-evoked twitches in a concentration-dependent manner. PYY 1-36 was more potent than any of the fragments. There was a tendency for shorter fragments to have lower pIC50 values. PYY 24-36 and 25-36 suppressed the twitches at high concentrations (3 microM). PYY 26-36 was without effect. 4. The findings suggest that the 12 C-terminal amino acid residues of NPY and PYY are the minimum length required to activate the Y2-receptor.</p>},
  author       = {Grundemar, L and Håkanson, R},
  issn         = {0007-1188},
  language     = {eng},
  number       = {1},
  pages        = {190--192},
  publisher    = {The British Pharmacological Society},
  series       = {British Journal of Pharmacology},
  title        = {Effects of various neuropeptide Y/peptide YY fragments on electrically-evoked contractions of the rat vas deferens},
  url          = {http://dx.doi.org/10.1111/j.1476-5381.1990.tb12075.x},
  doi          = {10.1111/j.1476-5381.1990.tb12075.x},
  volume       = {100},
  year         = {1990},
}