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Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds

Noppe, W.; Plieva, Fatima LU ; Vanhoorelbeke, K.; Deckmyn, H.; Tuncel, M.; Tuncel, A.; Galaev, Igor LU and Mattiasson, Bo LU (2007) In Journal of Biotechnology 131(3). p.293-299
Abstract
Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages... (More)
Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
von Willebrand factor, Lactoferrin, Protein purification, Bacteriophages, Cryogel, Macroporous hydrogel
in
Journal of Biotechnology
volume
131
issue
3
pages
293 - 299
publisher
Elsevier
external identifiers
  • wos:000250031300011
  • scopus:34548383766
ISSN
1873-4863
DOI
10.1016/j.jbiotec.2007.06.021
language
English
LU publication?
yes
id
4bcc0aef-64ca-4efd-9013-b038b0544353 (old id 792831)
date added to LUP
2008-01-04 15:05:12
date last changed
2017-11-19 03:29:15
@article{4bcc0aef-64ca-4efd-9013-b038b0544353,
  abstract     = {Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.},
  author       = {Noppe, W. and Plieva, Fatima and Vanhoorelbeke, K. and Deckmyn, H. and Tuncel, M. and Tuncel, A. and Galaev, Igor and Mattiasson, Bo},
  issn         = {1873-4863},
  keyword      = {von Willebrand factor,Lactoferrin,Protein purification,Bacteriophages,Cryogel,Macroporous hydrogel},
  language     = {eng},
  number       = {3},
  pages        = {293--299},
  publisher    = {Elsevier},
  series       = {Journal of Biotechnology},
  title        = {Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds},
  url          = {http://dx.doi.org/10.1016/j.jbiotec.2007.06.021},
  volume       = {131},
  year         = {2007},
}