Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds
(2007) In Journal of Biotechnology 131(3). p.293-299- Abstract
- Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages... (More)
- Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/792831
- author
- Noppe, W. ; Plieva, Fatima LU ; Vanhoorelbeke, K. ; Deckmyn, H. ; Tuncel, M. ; Tuncel, A. ; Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- von Willebrand factor, Lactoferrin, Protein purification, Bacteriophages, Cryogel, Macroporous hydrogel
- in
- Journal of Biotechnology
- volume
- 131
- issue
- 3
- pages
- 293 - 299
- publisher
- Elsevier
- external identifiers
-
- wos:000250031300011
- scopus:34548383766
- pmid:17709150
- ISSN
- 1873-4863
- DOI
- 10.1016/j.jbiotec.2007.06.021
- language
- English
- LU publication?
- yes
- id
- 4bcc0aef-64ca-4efd-9013-b038b0544353 (old id 792831)
- date added to LUP
- 2016-04-01 11:51:30
- date last changed
- 2022-03-13 01:44:51
@article{4bcc0aef-64ca-4efd-9013-b038b0544353,
abstract = {{Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10–100 μm) of macroporous poly(dimethylacrylamide) makes it possible to couple long (1 μm) phage particles as ligands without any risk of blocking the monolithic column. The macroporous monolithic columns were successfully used for the direct affinity capture of target proteins from particulate containing feeds like milk containing casein micelles and fat globules (1–10 μm in size) or even whole blood containing blood cells (up to 20 μm in size). The newly developed platform based on selected bacteriophages immobilized within macropores of the monolithic cryogels presents a convenient alternative to antibodies for fast and selective development of the specific adsorbent.}},
author = {{Noppe, W. and Plieva, Fatima and Vanhoorelbeke, K. and Deckmyn, H. and Tuncel, M. and Tuncel, A. and Galaev, Igor and Mattiasson, Bo}},
issn = {{1873-4863}},
keywords = {{von Willebrand factor; Lactoferrin; Protein purification; Bacteriophages; Cryogel; Macroporous hydrogel}},
language = {{eng}},
number = {{3}},
pages = {{293--299}},
publisher = {{Elsevier}},
series = {{Journal of Biotechnology}},
title = {{Macroporous monolithic gels, cryogels, with immobilized phages from phage-display library as a new platform for fast development of affinity adsorbent capable of target capture from crude feeds}},
url = {{http://dx.doi.org/10.1016/j.jbiotec.2007.06.021}},
doi = {{10.1016/j.jbiotec.2007.06.021}},
volume = {{131}},
year = {{2007}},
}