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Universal Method for Synthesis of Artificial Gel Antibodies by the Imprinting Approach Combined with a Unique Electrophoresis Technique for Detection of Minute Structural Differences of Proteins, Viruses and Cells (Bacteria) Ib. Gel Antibodies against Proteins (Hemoglobins)

Takátsy, Anikó; Végvári, Ákos LU ; Kilár, Ferenc and Hjertén, Stellan (2007) In Electrophoresis 28(14). p.2345-2350
Abstract
Using the molecular imprinting approach, we have shown that polyacrylamide-based artificial antibodies against human and bovine hemoglobin have a very high selectivity, as revealed by the free-zone electrophoresis in a revolving capillary. By the same technique we have previously synthesized gel antibodies not only against proteins but also against viruses and bacteria. The synthesis is thus universal, i.e., it has the great advantage of not requiring a modification – or only a slight one – for each particular antigen. The combination synthesis of artificial gel antibodies and electrophoretic analysis reveals small discrepancies in shape and chemical composition not only of proteins, as shown here and in paper Ia, but also of viruses and... (More)
Using the molecular imprinting approach, we have shown that polyacrylamide-based artificial antibodies against human and bovine hemoglobin have a very high selectivity, as revealed by the free-zone electrophoresis in a revolving capillary. By the same technique we have previously synthesized gel antibodies not only against proteins but also against viruses and bacteria. The synthesis is thus universal, i.e., it has the great advantage of not requiring a modification – or only a slight one – for each particular antigen. The combination synthesis of artificial gel antibodies and electrophoretic analysis reveals small discrepancies in shape and chemical composition not only of proteins, as shown here and in paper Ia, but also of viruses and bacteria, to be illustrated in papers II and III in this series. Upon rehydration,the freeze-dried gel antibodies, selective for human hemoglobin, regain their selectivity. The gel antibodies can repeatedly be used following the removal of the antigen (protein in this study) from the complex gel antibody/antigen by an SDS washing or an enzymatic degradation. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Artificial gel antibodies / Free-zone electrophoresis / Gel antibodies / Hemoglobin / Molecular imprinting
in
Electrophoresis
volume
28
issue
14
pages
2345 - 2350
publisher
John Wiley & Sons
external identifiers
  • scopus:34547474113
ISSN
0173-0835
DOI
10.1002/elps.200600191
language
English
LU publication?
no
id
cfa0ed0f-0368-407d-a235-965343425a97 (old id 793767)
date added to LUP
2008-01-08 14:38:30
date last changed
2017-01-08 05:40:46
@article{cfa0ed0f-0368-407d-a235-965343425a97,
  abstract     = {Using the molecular imprinting approach, we have shown that polyacrylamide-based artificial antibodies against human and bovine hemoglobin have a very high selectivity, as revealed by the free-zone electrophoresis in a revolving capillary. By the same technique we have previously synthesized gel antibodies not only against proteins but also against viruses and bacteria. The synthesis is thus universal, i.e., it has the great advantage of not requiring a modification – or only a slight one – for each particular antigen. The combination synthesis of artificial gel antibodies and electrophoretic analysis reveals small discrepancies in shape and chemical composition not only of proteins, as shown here and in paper Ia, but also of viruses and bacteria, to be illustrated in papers II and III in this series. Upon rehydration,the freeze-dried gel antibodies, selective for human hemoglobin, regain their selectivity. The gel antibodies can repeatedly be used following the removal of the antigen (protein in this study) from the complex gel antibody/antigen by an SDS washing or an enzymatic degradation.},
  author       = {Takátsy, Anikó and Végvári, Ákos and Kilár, Ferenc and Hjertén, Stellan},
  issn         = {0173-0835},
  keyword      = {Artificial gel antibodies / Free-zone electrophoresis / Gel antibodies / Hemoglobin / Molecular imprinting},
  language     = {eng},
  number       = {14},
  pages        = {2345--2350},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Universal Method for Synthesis of Artificial Gel Antibodies by the Imprinting Approach Combined with a Unique Electrophoresis Technique for Detection of Minute Structural Differences of Proteins, Viruses and Cells (Bacteria) Ib. Gel Antibodies against Proteins (Hemoglobins)},
  url          = {http://dx.doi.org/10.1002/elps.200600191},
  volume       = {28},
  year         = {2007},
}