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Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes

Tarnawski, Laura LU ; Xian, Xiaojie LU ; Monnerat, Gustavo ; Macaulay, Iain C. ; Malan, Daniela ; Borgman, Andrew ; Wu, Sean M. ; Fleischmann, Bernd K. and Jovinge, Stefan LU (2015) In PLoS ONE 10(8).
Abstract
In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity fromvarious differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellularmarkers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surfacemarker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand... (More)
In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity fromvarious differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellularmarkers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surfacemarker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell expression profiling in early mouse embryonic hearts, we found that a combination of integrin alpha-1, alpha-5, alpha-6 and N-cadherin enables isolation of lineage committed murine cardiomyocytes. Additionally, we were able to separate trabecular cardiomyocytes from solid ventricular myocardium and atrial murine cells. These cells exhibit expected subtype specific phenotype confirmed by electrophysiological analysis. We show that integrin expression can be used for the isolation of living, functional and lineage-specific murine cardiomyocytes. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
10
issue
8
article number
e0135880
publisher
Public Library of Science (PLoS)
external identifiers
  • wos:000360435500010
  • scopus:84943279639
  • pmid:26323090
  • pmid:26323090
ISSN
1932-6203
DOI
10.1371/journal.pone.0135880
language
English
LU publication?
yes
id
e114080b-d800-4e5a-9168-353d6c5b3efd (old id 7964889)
date added to LUP
2016-04-01 15:01:08
date last changed
2022-07-31 01:06:03
@article{e114080b-d800-4e5a-9168-353d6c5b3efd,
  abstract     = {{In contrast to mature cardiomyocytes which have limited regenerative capacity, pluripotent stem cells represent a promising source for the generation of new cardiomyocytes. The tendency of pluripotent stem cells to form teratomas and the heterogeneity fromvarious differentiation stages and cardiomyocyte cell sub-types, however, are major obstacles to overcome before this type of therapy could be applied in a clinical setting. Thus, the identification of extracellularmarkers for specific cardiomyocyte progenitors and mature subpopulations is of particular importance. The delineation of cardiomyocyte surfacemarker patterns not only serves as a means to derive homogeneous cell populations by FACS, but is also an essential tool to understand cardiac development. By using single-cell expression profiling in early mouse embryonic hearts, we found that a combination of integrin alpha-1, alpha-5, alpha-6 and N-cadherin enables isolation of lineage committed murine cardiomyocytes. Additionally, we were able to separate trabecular cardiomyocytes from solid ventricular myocardium and atrial murine cells. These cells exhibit expected subtype specific phenotype confirmed by electrophysiological analysis. We show that integrin expression can be used for the isolation of living, functional and lineage-specific murine cardiomyocytes.}},
  author       = {{Tarnawski, Laura and Xian, Xiaojie and Monnerat, Gustavo and Macaulay, Iain C. and Malan, Daniela and Borgman, Andrew and Wu, Sean M. and Fleischmann, Bernd K. and Jovinge, Stefan}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  number       = {{8}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{Integrin Based Isolation Enables Purification of Murine Lineage Committed Cardiomyocytes}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0135880}},
  doi          = {{10.1371/journal.pone.0135880}},
  volume       = {{10}},
  year         = {{2015}},
}