A rat model of otitis media with effusion caused by eustachian tube obstruction with and without Streptococcus pneumoniae infection : Methods and course
Piltcher, Otavio B. ; Swarts, J. Douglas ; Magnuson, Karin LU
; Alper, Cuneyt M.
; Doyle, William J.
and Hebda, Patricia A.
(2002)
In Otolaryngology - Head and Neck Surgery
126(5).
p.490-498
- Abstract
OBJECTIVE: To describe the clinical and histopathologic progression of a rat model of otitis media with effusion caused by eustachian tube obstruction (ETO) with and without Streptococcus pneumoniae infection. METHODS: In 164 rats, the left, bony eustachian tube was approached via a ventral incision and obstructed with dental material. Then 108 rats were infected via an intrabullar injection with S pneumoniae. At 48 hours, the infected rats were treated for 5 days with ampicillin. All ears were evaluated by weekly otomicroscopy. On each of days 1, 2, 7, 21, 35, 56, and 112, four rats were killed for histologic study. All effusions were cultured for bacteria. RESULTS: Fourteen rats died of surgical complications; effusion resolved by 2... (More)
OBJECTIVE: To describe the clinical and histopathologic progression of a rat model of otitis media with effusion caused by eustachian tube obstruction (ETO) with and without Streptococcus pneumoniae infection. METHODS: In 164 rats, the left, bony eustachian tube was approached via a ventral incision and obstructed with dental material. Then 108 rats were infected via an intrabullar injection with S pneumoniae. At 48 hours, the infected rats were treated for 5 days with ampicillin. All ears were evaluated by weekly otomicroscopy. On each of days 1, 2, 7, 21, 35, 56, and 112, four rats were killed for histologic study. All effusions were cultured for bacteria. RESULTS: Fourteen rats died of surgical complications; effusion resolved by 2 weeks in 9 rats. During the first few days, infected ears with ETO had bulging tympanic membranes, followed by tympanic membrane retraction, purulent effusion, and otorrhea (50%) over the next few weeks, whereas uninfecfed ears with ETO developed retraction and serous effusion during the same time frame. At later times, all ears with ETO presented with retraction and serous or serous-mucoid effusion. S pneumoniae was recovered only from the infected ears with ETO (days 1 and 2), with some colonization by non-pathogenic microorganisms observed equally in both groups of ears. Histology showed a typical acute inflammatory reaction in the challenged ears with ETO through day 14 and then a chronic inflammation for all ears with ETO. CONCLUSION: The experimental methods provoked reproducible pathologic signs similar to those for otitis media with effusion. Given the availability of rat-specific reagents, this model is well suited for studies of cytokine elaboration during disease pathogenesis.
(Less)
- author
- Piltcher, Otavio B.
; Swarts, J. Douglas
; Magnuson, Karin
LU
; Alper, Cuneyt M.
; Doyle, William J.
and Hebda, Patricia A.
- publishing date
- 2002-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Otolaryngology - Head and Neck Surgery
- volume
- 126
- issue
- 5
- pages
- 9 pages
- publisher
- Mosby-Elsevier
- external identifiers
-
- pmid:12075222
- scopus:0036561283
- ISSN
- 0194-5998
- DOI
- 10.1067/mhn.2002.124935
- language
- English
- LU publication?
- no
- id
- 79681b78-8ab8-42a9-9e84-f978616802cd
- date added to LUP
- 2019-05-08 13:02:52
- date last changed
- 2024-04-02 01:38:35
@article{79681b78-8ab8-42a9-9e84-f978616802cd,
abstract = {{<p>OBJECTIVE: To describe the clinical and histopathologic progression of a rat model of otitis media with effusion caused by eustachian tube obstruction (ETO) with and without Streptococcus pneumoniae infection. METHODS: In 164 rats, the left, bony eustachian tube was approached via a ventral incision and obstructed with dental material. Then 108 rats were infected via an intrabullar injection with S pneumoniae. At 48 hours, the infected rats were treated for 5 days with ampicillin. All ears were evaluated by weekly otomicroscopy. On each of days 1, 2, 7, 21, 35, 56, and 112, four rats were killed for histologic study. All effusions were cultured for bacteria. RESULTS: Fourteen rats died of surgical complications; effusion resolved by 2 weeks in 9 rats. During the first few days, infected ears with ETO had bulging tympanic membranes, followed by tympanic membrane retraction, purulent effusion, and otorrhea (50%) over the next few weeks, whereas uninfecfed ears with ETO developed retraction and serous effusion during the same time frame. At later times, all ears with ETO presented with retraction and serous or serous-mucoid effusion. S pneumoniae was recovered only from the infected ears with ETO (days 1 and 2), with some colonization by non-pathogenic microorganisms observed equally in both groups of ears. Histology showed a typical acute inflammatory reaction in the challenged ears with ETO through day 14 and then a chronic inflammation for all ears with ETO. CONCLUSION: The experimental methods provoked reproducible pathologic signs similar to those for otitis media with effusion. Given the availability of rat-specific reagents, this model is well suited for studies of cytokine elaboration during disease pathogenesis.</p>}},
author = {{Piltcher, Otavio B. and Swarts, J. Douglas and Magnuson, Karin and Alper, Cuneyt M. and Doyle, William J. and Hebda, Patricia A.}},
issn = {{0194-5998}},
language = {{eng}},
month = {{01}},
number = {{5}},
pages = {{490--498}},
publisher = {{Mosby-Elsevier}},
series = {{Otolaryngology - Head and Neck Surgery}},
title = {{A rat model of otitis media with effusion caused by eustachian tube obstruction with and without Streptococcus pneumoniae infection : Methods and course}},
url = {{http://dx.doi.org/10.1067/mhn.2002.124935}},
doi = {{10.1067/mhn.2002.124935}},
volume = {{126}},
year = {{2002}},
}