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miR-124 inhibits cell proliferation in breast cancer through downregulation of CDK4

Feng, Tongbao; Xu, Dongqin; Tu, Chao; Li, Wenjing; Ning, Yongling; Ding, Jun; Wang, Shizhong; Yuan, Liudi; Xu, Ning LU and Qian, Keqing, et al. (2015) In Tumor Biology 36(8). p.5987-5997
Abstract
Studies have shown that microRNAs (miRNAs) are involved in the malignant progression of human cancer. However, little is known about the potential role of miRNAs in breast carcinogenesis. miR-124 expression in breast cancer tissue was measured by quantitative real-time PCR (qRT-PCR). Target prediction algorithms and luciferase reporter gene assays were used to investigate the target of miR-124. Breast cancer cells growth was regulated by overexpression or knockdown miR-124. At the end of the study, tumor-bearing mice were tested to confirm the function of miR-124 in breast cancer. In this study, we demonstrated that the expression of miR-124 was significantly downregulated in breast cancer tissues compared with matched adjacent... (More)
Studies have shown that microRNAs (miRNAs) are involved in the malignant progression of human cancer. However, little is known about the potential role of miRNAs in breast carcinogenesis. miR-124 expression in breast cancer tissue was measured by quantitative real-time PCR (qRT-PCR). Target prediction algorithms and luciferase reporter gene assays were used to investigate the target of miR-124. Breast cancer cells growth was regulated by overexpression or knockdown miR-124. At the end of the study, tumor-bearing mice were tested to confirm the function of miR-124 in breast cancer. In this study, we demonstrated that the expression of miR-124 was significantly downregulated in breast cancer tissues compared with matched adjacent non-neoplastic tissues. We identified and confirmed that cyclin-dependent kinase 4 (CDK4) was a direct target of miR-124. Overexpression of miR-124 suppressed CDK4 protein expression and attenuated cell viability, proliferation, and cell cycle progression in MCF-7 and MDA-MB-435S breast cancer cells in vitro. Overexpression of CDK4 partially rescued the inhibitory effect of miR-124 in the breast cancer cells. Moreover, we found that miR-124 overexpression effectively repressed tumor growth in xenograft animal experiments. Our results demonstrate that miR-124 functions as a growth-suppressive miRNA and plays an important role in inhibiting tumorigenesis by targeting CDK4. (Less)
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type
Contribution to journal
publication status
published
subject
keywords
Cell cycle, Proliferation, Breast cancer, CDK4, miR-124
in
Tumor Biology
volume
36
issue
8
pages
5987 - 5997
publisher
Springer
external identifiers
  • wos:000360193800033
  • scopus:84940436891
ISSN
1423-0380
DOI
10.1007/s13277-015-3275-8
language
English
LU publication?
yes
id
5c2742b0-9705-4935-9c81-740e857f0d87 (old id 7969160)
date added to LUP
2015-10-01 07:37:39
date last changed
2017-10-22 04:01:36
@article{5c2742b0-9705-4935-9c81-740e857f0d87,
  abstract     = {Studies have shown that microRNAs (miRNAs) are involved in the malignant progression of human cancer. However, little is known about the potential role of miRNAs in breast carcinogenesis. miR-124 expression in breast cancer tissue was measured by quantitative real-time PCR (qRT-PCR). Target prediction algorithms and luciferase reporter gene assays were used to investigate the target of miR-124. Breast cancer cells growth was regulated by overexpression or knockdown miR-124. At the end of the study, tumor-bearing mice were tested to confirm the function of miR-124 in breast cancer. In this study, we demonstrated that the expression of miR-124 was significantly downregulated in breast cancer tissues compared with matched adjacent non-neoplastic tissues. We identified and confirmed that cyclin-dependent kinase 4 (CDK4) was a direct target of miR-124. Overexpression of miR-124 suppressed CDK4 protein expression and attenuated cell viability, proliferation, and cell cycle progression in MCF-7 and MDA-MB-435S breast cancer cells in vitro. Overexpression of CDK4 partially rescued the inhibitory effect of miR-124 in the breast cancer cells. Moreover, we found that miR-124 overexpression effectively repressed tumor growth in xenograft animal experiments. Our results demonstrate that miR-124 functions as a growth-suppressive miRNA and plays an important role in inhibiting tumorigenesis by targeting CDK4.},
  author       = {Feng, Tongbao and Xu, Dongqin and Tu, Chao and Li, Wenjing and Ning, Yongling and Ding, Jun and Wang, Shizhong and Yuan, Liudi and Xu, Ning and Qian, Keqing and Wang, Yong and Qi, Chunjian},
  issn         = {1423-0380},
  keyword      = {Cell cycle,Proliferation,Breast cancer,CDK4,miR-124},
  language     = {eng},
  number       = {8},
  pages        = {5987--5997},
  publisher    = {Springer},
  series       = {Tumor Biology},
  title        = {miR-124 inhibits cell proliferation in breast cancer through downregulation of CDK4},
  url          = {http://dx.doi.org/10.1007/s13277-015-3275-8},
  volume       = {36},
  year         = {2015},
}