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IgG and fibrinogen driven nanoparticle aggregation

Cukalevski, Risto LU ; Ferreira, Silvia A.; Dunning, Christopher LU ; Berggard, Tord and Cedervall, Tommy LU (2015) In Nano Reseach 8(8). p.2733-2743
Abstract
A thorough understanding of how proteins induce nanoparticle (NP) aggregation is crucial when designing in vitro and in vivo assays and interpreting experimental results. This knowledge is also crucial when developing nano-applications and formulation for drug delivery systems. In this study, we found that extraction of immunoglobulin G (IgG) from cow serum results in lower polystyrene NPs aggregation. Moreover, addition of isolated IgG or fibrinogen to fetal cow serum enhanced this aggregation, thus demonstrating that these factors are major drivers of NP aggregation in serum. Counter-intuitively, NP aggregation was inversely dependent on protein concentration; i.e., low protein concentrations induced large aggregates, whereas high... (More)
A thorough understanding of how proteins induce nanoparticle (NP) aggregation is crucial when designing in vitro and in vivo assays and interpreting experimental results. This knowledge is also crucial when developing nano-applications and formulation for drug delivery systems. In this study, we found that extraction of immunoglobulin G (IgG) from cow serum results in lower polystyrene NPs aggregation. Moreover, addition of isolated IgG or fibrinogen to fetal cow serum enhanced this aggregation, thus demonstrating that these factors are major drivers of NP aggregation in serum. Counter-intuitively, NP aggregation was inversely dependent on protein concentration; i.e., low protein concentrations induced large aggregates, whereas high protein concentrations induced small aggregates. Protein-induced NP aggregation and aggregate size were monitored by absorbance at 400 nm and dynamic light scattering, respectively. Here, we propose a mechanism behind the protein concentration dependent aggregation; this mechanism involves the effects of multiple protein interactions on the NP surface, surface area limitations, aggregation kinetics, and the influence of other serum proteins. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunoglobulin, aggregation, corona, protein, nanoparticles (NPs)
in
Nano Reseach
volume
8
issue
8
pages
2733 - 2743
publisher
Springer
external identifiers
  • wos:000359865100026
  • scopus:84939267321
ISSN
1998-0124
DOI
10.1007/s12274-015-0780-4
language
English
LU publication?
yes
id
a045e4d4-c85e-4911-af24-a8ad00b8a794 (old id 7972329)
date added to LUP
2015-09-24 17:40:37
date last changed
2017-09-03 03:07:39
@article{a045e4d4-c85e-4911-af24-a8ad00b8a794,
  abstract     = {A thorough understanding of how proteins induce nanoparticle (NP) aggregation is crucial when designing in vitro and in vivo assays and interpreting experimental results. This knowledge is also crucial when developing nano-applications and formulation for drug delivery systems. In this study, we found that extraction of immunoglobulin G (IgG) from cow serum results in lower polystyrene NPs aggregation. Moreover, addition of isolated IgG or fibrinogen to fetal cow serum enhanced this aggregation, thus demonstrating that these factors are major drivers of NP aggregation in serum. Counter-intuitively, NP aggregation was inversely dependent on protein concentration; i.e., low protein concentrations induced large aggregates, whereas high protein concentrations induced small aggregates. Protein-induced NP aggregation and aggregate size were monitored by absorbance at 400 nm and dynamic light scattering, respectively. Here, we propose a mechanism behind the protein concentration dependent aggregation; this mechanism involves the effects of multiple protein interactions on the NP surface, surface area limitations, aggregation kinetics, and the influence of other serum proteins.},
  author       = {Cukalevski, Risto and Ferreira, Silvia A. and Dunning, Christopher and Berggard, Tord and Cedervall, Tommy},
  issn         = {1998-0124},
  keyword      = {immunoglobulin,aggregation,corona,protein,nanoparticles (NPs)},
  language     = {eng},
  number       = {8},
  pages        = {2733--2743},
  publisher    = {Springer},
  series       = {Nano Reseach},
  title        = {IgG and fibrinogen driven nanoparticle aggregation},
  url          = {http://dx.doi.org/10.1007/s12274-015-0780-4},
  volume       = {8},
  year         = {2015},
}