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MHC class II B diversity in blue tits: a preliminary study

Rivero-de Aguilar, Juan; Schut, Elske; Merino, Santiago; Martinez, Javier; Komdeur, Jan and Westerdahl, Helena LU (2013) In Ecology and Evolution 3(7). p.1878-1889
Abstract
In this study, we partly characterize major histocompatibility complex (MHC) class II B in the blue tit (Cyanistes caeruleus). A total of 22 individuals from three different European locations: Spain, The Netherlands, and Sweden were screened for MHC allelic diversity. The MHC genes were investigated using both PCR-based methods and unamplified genomic DNA with restriction fragment length polymorphism (RFLP) and southern blots. A total of 13 different exon 2 sequences were obtained independently from DNA and/or RNA, thus confirming gene transcription and likely functionality of the genes. Nine out of 13 alleles were found in more than one country, and two alleles appeared in all countries. Positive selection was detected in the region... (More)
In this study, we partly characterize major histocompatibility complex (MHC) class II B in the blue tit (Cyanistes caeruleus). A total of 22 individuals from three different European locations: Spain, The Netherlands, and Sweden were screened for MHC allelic diversity. The MHC genes were investigated using both PCR-based methods and unamplified genomic DNA with restriction fragment length polymorphism (RFLP) and southern blots. A total of 13 different exon 2 sequences were obtained independently from DNA and/or RNA, thus confirming gene transcription and likely functionality of the genes. Nine out of 13 alleles were found in more than one country, and two alleles appeared in all countries. Positive selection was detected in the region coding for the peptide binding region (PBR). A maximum of three alleles per individual was detected by sequencing and the RFLP pattern consisted of 4-7 fragments, indicating a minimum number of 2-4 loci per individual. A phylogenetic analysis, demonstrated that the blue tit sequences are divergent compared to sequences from other passerines resembling a different MHC lineage than those possessed by most passerines studied to date. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Cyanistes caeruleus, major histocompatibility complex, Parus caeruleus, passerine
in
Ecology and Evolution
volume
3
issue
7
pages
1878 - 1889
publisher
Wiley-Blackwell
external identifiers
  • wos:000322002400005
  • scopus:84886297707
ISSN
2045-7758
DOI
10.1002/ece3.598
language
English
LU publication?
yes
id
79778a0b-587e-40f0-8ffe-6a534bf73833 (old id 4053407)
date added to LUP
2013-09-19 11:13:16
date last changed
2019-02-20 06:44:41
@article{79778a0b-587e-40f0-8ffe-6a534bf73833,
  abstract     = {In this study, we partly characterize major histocompatibility complex (MHC) class II B in the blue tit (Cyanistes caeruleus). A total of 22 individuals from three different European locations: Spain, The Netherlands, and Sweden were screened for MHC allelic diversity. The MHC genes were investigated using both PCR-based methods and unamplified genomic DNA with restriction fragment length polymorphism (RFLP) and southern blots. A total of 13 different exon 2 sequences were obtained independently from DNA and/or RNA, thus confirming gene transcription and likely functionality of the genes. Nine out of 13 alleles were found in more than one country, and two alleles appeared in all countries. Positive selection was detected in the region coding for the peptide binding region (PBR). A maximum of three alleles per individual was detected by sequencing and the RFLP pattern consisted of 4-7 fragments, indicating a minimum number of 2-4 loci per individual. A phylogenetic analysis, demonstrated that the blue tit sequences are divergent compared to sequences from other passerines resembling a different MHC lineage than those possessed by most passerines studied to date.},
  author       = {Rivero-de Aguilar, Juan and Schut, Elske and Merino, Santiago and Martinez, Javier and Komdeur, Jan and Westerdahl, Helena},
  issn         = {2045-7758},
  keyword      = {Cyanistes caeruleus,major histocompatibility complex,Parus caeruleus,passerine},
  language     = {eng},
  number       = {7},
  pages        = {1878--1889},
  publisher    = {Wiley-Blackwell},
  series       = {Ecology and Evolution},
  title        = {MHC class II B diversity in blue tits: a preliminary study},
  url          = {http://dx.doi.org/10.1002/ece3.598},
  volume       = {3},
  year         = {2013},
}