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Active uptake of kleptoplastids by Dinophysis caudata from its ciliate prey Myrionecta rubra

Minnhagen, S.; Kim, M.; Salomon, P. S.; Yih, W.; Graneli, Edna LU and Park, M. G. (2011) In Aquatic Microbial Ecology 62(1). p.99-108
Abstract
We verified an active uptake of kleptoplastids in the toxic and bloom-forming dinoflagellates of the genus Dinophysis from its preferred prey, the ciliate Myrionecta rubra, using a quantitative real-time PCR technique. During a 65 d starvation/feeding experiment with Dinophysis caudata, changes in plastid 16S rRNA, plastid autofluorescence and plastid/nuclear DNA ratio through the cell cycle were followed with quantitative real-time PCR and flow cytometry. During starvation, the cultures initially showed a rapid growth and a 3.5-fold increase of number of cells ml(-1), while at the same time, plastid DNA cell(-1) showed a 3.5-fold decrease, and a 3.6-fold decrease in phycoerythrin fluorescence cell(-1). The decrease in plastid DNA cell(-1)... (More)
We verified an active uptake of kleptoplastids in the toxic and bloom-forming dinoflagellates of the genus Dinophysis from its preferred prey, the ciliate Myrionecta rubra, using a quantitative real-time PCR technique. During a 65 d starvation/feeding experiment with Dinophysis caudata, changes in plastid 16S rRNA, plastid autofluorescence and plastid/nuclear DNA ratio through the cell cycle were followed with quantitative real-time PCR and flow cytometry. During starvation, the cultures initially showed a rapid growth and a 3.5-fold increase of number of cells ml(-1), while at the same time, plastid DNA cell(-1) showed a 3.5-fold decrease, and a 3.6-fold decrease in phycoerythrin fluorescence cell(-1). The decrease in plastid DNA cell(-1) d(-1) closely followed culture growth rate (Pearson correlation, r = 0.91), indicating that existing plastids were diluted within the growing population and that no new plastids were synthesised by the cells. When starved cells were re-fed by the ciliate M. rubra on Days 43 to 51 of the experiment, plastid DNA cell(-1) increased 7-fold up to 14 000 16S DNA copies per cell, thereby directly revealing the kleptoplastic behaviour. The implication is that not only availability of the prey M. rubra itself, but also the supply of suitable kleptoplastids might be an important controlling factor for Dinophysis spp. bloom formation and decline. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
categories
Higher Education
in
Aquatic Microbial Ecology
volume
62
issue
1
pages
99 - 108
publisher
Inter-Research
external identifiers
  • scopus:78651366703
ISSN
0948-3055
DOI
10.3354/ame01459
language
English
LU publication?
no
id
22b4bd35-56c4-48ab-8d43-d2b04b762cd9 (old id 7993871)
date added to LUP
2015-09-29 13:22:39
date last changed
2017-05-28 03:51:52
@article{22b4bd35-56c4-48ab-8d43-d2b04b762cd9,
  abstract     = {We verified an active uptake of kleptoplastids in the toxic and bloom-forming dinoflagellates of the genus Dinophysis from its preferred prey, the ciliate Myrionecta rubra, using a quantitative real-time PCR technique. During a 65 d starvation/feeding experiment with Dinophysis caudata, changes in plastid 16S rRNA, plastid autofluorescence and plastid/nuclear DNA ratio through the cell cycle were followed with quantitative real-time PCR and flow cytometry. During starvation, the cultures initially showed a rapid growth and a 3.5-fold increase of number of cells ml(-1), while at the same time, plastid DNA cell(-1) showed a 3.5-fold decrease, and a 3.6-fold decrease in phycoerythrin fluorescence cell(-1). The decrease in plastid DNA cell(-1) d(-1) closely followed culture growth rate (Pearson correlation, r = 0.91), indicating that existing plastids were diluted within the growing population and that no new plastids were synthesised by the cells. When starved cells were re-fed by the ciliate M. rubra on Days 43 to 51 of the experiment, plastid DNA cell(-1) increased 7-fold up to 14 000 16S DNA copies per cell, thereby directly revealing the kleptoplastic behaviour. The implication is that not only availability of the prey M. rubra itself, but also the supply of suitable kleptoplastids might be an important controlling factor for Dinophysis spp. bloom formation and decline.},
  author       = {Minnhagen, S. and Kim, M. and Salomon, P. S. and Yih, W. and Graneli, Edna and Park, M. G.},
  issn         = {0948-3055},
  language     = {eng},
  number       = {1},
  pages        = {99--108},
  publisher    = {Inter-Research},
  series       = {Aquatic Microbial Ecology},
  title        = {Active uptake of kleptoplastids by Dinophysis caudata from its ciliate prey Myrionecta rubra},
  url          = {http://dx.doi.org/10.3354/ame01459},
  volume       = {62},
  year         = {2011},
}