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Eosinophils are key regulators of perivascular adipose tissue and vascular functionality

Withers, Sarah B.; Forman, Ruth; Meza-Perez, Selene; Sorobetea, Daniel LU ; Sitnik, Kasia LU ; Hopwood, Thomas; Lawrence, Catherine B.; Agace, William W. LU ; Else, Kathryn J. and Heagerty, Anthony M, et al. (2017) In Scientific Reports 7.
Abstract

Obesity impairs the relaxant capacity of adipose tissue surrounding the vasculature (PVAT) and has been implicated in resultant obesity-related hypertension and impaired glucose intolerance. Resident immune cells are thought to regulate adipocyte activity. We investigated the role of eosinophils in mediating normal PVAT function. Healthy PVAT elicits an anti-contractile effect, which was lost in mice deficient in eosinophils, mimicking the obese phenotype, and was restored upon eosinophil reconstitution. Ex vivo studies demonstrated that the loss of PVAT function was due to reduced bioavailability of adiponectin and adipocyte-derived nitric oxide, which was restored after eosinophil reconstitution. Mechanistic studies demonstrated that... (More)

Obesity impairs the relaxant capacity of adipose tissue surrounding the vasculature (PVAT) and has been implicated in resultant obesity-related hypertension and impaired glucose intolerance. Resident immune cells are thought to regulate adipocyte activity. We investigated the role of eosinophils in mediating normal PVAT function. Healthy PVAT elicits an anti-contractile effect, which was lost in mice deficient in eosinophils, mimicking the obese phenotype, and was restored upon eosinophil reconstitution. Ex vivo studies demonstrated that the loss of PVAT function was due to reduced bioavailability of adiponectin and adipocyte-derived nitric oxide, which was restored after eosinophil reconstitution. Mechanistic studies demonstrated that adiponectin and nitric oxide are released after activation of adipocyte-expressed β3 adrenoceptors by catecholamines, and identified eosinophils as a novel source of these mediators. We conclude that adipose tissue eosinophils play a key role in the regulation of normal PVAT anti-contractile function.

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Scientific Reports
volume
7
publisher
Nature Publishing Group
external identifiers
  • scopus:85015293079
  • wos:000397002100001
ISSN
2045-2322
DOI
10.1038/srep44571
language
English
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yes
id
7993fe40-2913-4685-9bca-06021ecd9a00
date added to LUP
2017-03-29 11:15:32
date last changed
2017-09-18 11:34:21
@article{7993fe40-2913-4685-9bca-06021ecd9a00,
  abstract     = {<p>Obesity impairs the relaxant capacity of adipose tissue surrounding the vasculature (PVAT) and has been implicated in resultant obesity-related hypertension and impaired glucose intolerance. Resident immune cells are thought to regulate adipocyte activity. We investigated the role of eosinophils in mediating normal PVAT function. Healthy PVAT elicits an anti-contractile effect, which was lost in mice deficient in eosinophils, mimicking the obese phenotype, and was restored upon eosinophil reconstitution. Ex vivo studies demonstrated that the loss of PVAT function was due to reduced bioavailability of adiponectin and adipocyte-derived nitric oxide, which was restored after eosinophil reconstitution. Mechanistic studies demonstrated that adiponectin and nitric oxide are released after activation of adipocyte-expressed β3 adrenoceptors by catecholamines, and identified eosinophils as a novel source of these mediators. We conclude that adipose tissue eosinophils play a key role in the regulation of normal PVAT anti-contractile function.</p>},
  articleno    = {44571},
  author       = {Withers, Sarah B. and Forman, Ruth and Meza-Perez, Selene and Sorobetea, Daniel and Sitnik, Kasia and Hopwood, Thomas and Lawrence, Catherine B. and Agace, William W. and Else, Kathryn J. and Heagerty, Anthony M and Svensson-Frej, Marcus and Cruickshank, Sheena M.},
  issn         = {2045-2322},
  language     = {eng},
  month        = {03},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Eosinophils are key regulators of perivascular adipose tissue and vascular functionality},
  url          = {http://dx.doi.org/10.1038/srep44571},
  volume       = {7},
  year         = {2017},
}