Studies of host-graft interactions in vitro.
(2007) In Journal of Neural Engineering 4(3). p.255-263- Abstract
- Progenitor and stem cell transplantation represent therapeutic strategies for retinal disorders that are accompanied by photoreceptor degeneration. The transplanted cells may either replace degenerating photoreceptors or secrete beneficial factors that halt the processes of photoreceptor degeneration. The present study analyzes whether rat retinal progenitor cells differentiated into photoreceptor phenotypic cells in neurospheres have a potential to interact with rat retinal explants. Immunocytochemistry for rhodopsin and synaptophysin indicated photoreceptor cell-like differentiation in neurospheres that were stimulated by basic fibroblast growth factor and epidermal growth factor. Differentiation into neural phenotypes including... (More)
- Progenitor and stem cell transplantation represent therapeutic strategies for retinal disorders that are accompanied by photoreceptor degeneration. The transplanted cells may either replace degenerating photoreceptors or secrete beneficial factors that halt the processes of photoreceptor degeneration. The present study analyzes whether rat retinal progenitor cells differentiated into photoreceptor phenotypic cells in neurospheres have a potential to interact with rat retinal explants. Immunocytochemistry for rhodopsin and synaptophysin indicated photoreceptor cell-like differentiation in neurospheres that were stimulated by basic fibroblast growth factor and epidermal growth factor. Differentiation into neural phenotypes including photoreceptor cells was effectively blocked by an addition of leukemia inhibitory factor. Grafting of neurospheres onto retinal explants demonstrated a consistent penetration of glial cell processes into the explanted tissue. On the other hand, the incorporation of donor cells into explants was very low. A general finding was that neurospheres grafting was associated with local decrease in Muller cell activation in the explants. Further characterization of these effect(s) could provide further insight into progenitor cell-based therapies of retinal degenerative disorders. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/607715
- author
- Liljekvist Soltic, Ingela LU and Johansson, Kjell
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Neural Engineering
- volume
- 4
- issue
- 3
- pages
- 255 - 263
- publisher
- IOP Publishing
- external identifiers
-
- wos:000250181600018
- scopus:48949097873
- pmid:17873428
- ISSN
- 1741-2560
- DOI
- 10.1088/1741-2560/4/3/011
- language
- English
- LU publication?
- yes
- id
- 79d13a88-3077-4f00-a1a2-ed585e8d5f65 (old id 607715)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17873428&dopt=Abstract
- date added to LUP
- 2016-04-01 11:53:31
- date last changed
- 2022-03-28 17:11:55
@article{79d13a88-3077-4f00-a1a2-ed585e8d5f65,
abstract = {{Progenitor and stem cell transplantation represent therapeutic strategies for retinal disorders that are accompanied by photoreceptor degeneration. The transplanted cells may either replace degenerating photoreceptors or secrete beneficial factors that halt the processes of photoreceptor degeneration. The present study analyzes whether rat retinal progenitor cells differentiated into photoreceptor phenotypic cells in neurospheres have a potential to interact with rat retinal explants. Immunocytochemistry for rhodopsin and synaptophysin indicated photoreceptor cell-like differentiation in neurospheres that were stimulated by basic fibroblast growth factor and epidermal growth factor. Differentiation into neural phenotypes including photoreceptor cells was effectively blocked by an addition of leukemia inhibitory factor. Grafting of neurospheres onto retinal explants demonstrated a consistent penetration of glial cell processes into the explanted tissue. On the other hand, the incorporation of donor cells into explants was very low. A general finding was that neurospheres grafting was associated with local decrease in Muller cell activation in the explants. Further characterization of these effect(s) could provide further insight into progenitor cell-based therapies of retinal degenerative disorders.}},
author = {{Liljekvist Soltic, Ingela and Johansson, Kjell}},
issn = {{1741-2560}},
language = {{eng}},
number = {{3}},
pages = {{255--263}},
publisher = {{IOP Publishing}},
series = {{Journal of Neural Engineering}},
title = {{Studies of host-graft interactions in vitro.}},
url = {{http://dx.doi.org/10.1088/1741-2560/4/3/011}},
doi = {{10.1088/1741-2560/4/3/011}},
volume = {{4}},
year = {{2007}},
}