Intra-individual short-term variability of prostate-specific antigen and other kallikrein markers in a serial collection of blood from men under evaluation for prostate cancer.
Christensson, Anders LU ; Bruun, Laila LU ; Björk, Thomas LU ; Cronin, Angel M ; Vickers, Andrew J ; Savage, Caroline J and Lilja, Hans LU
(2011)
In BJU International
107.
p.1769-1774
- Abstract
- Study Type - Diagnostic (exploratory cohort) Level of Evidence 2b OBJECTIVES: To assess variation of total prostate-specific antigen (tPSA), free PSA (fPSA), percent fPSA, human glandular kallikrein 2 (hK2) and intact PSA measured three times within 2 weeks. Knowledge of the variation in an individual's PSA level is important for clinical decision-making. PATIENTS AND METHODS: Study participants were 149 patients referred for prostate biopsy, of which 97 had benign disease and 52 had prostate cancer. Three blood samples were drawn with a median of 4 h between first and second samples and 12 days between first and third samples. Variability was described by absolute differences, ratios and intra-individual coefficients of variation. Total... (More)
- Study Type - Diagnostic (exploratory cohort) Level of Evidence 2b OBJECTIVES: To assess variation of total prostate-specific antigen (tPSA), free PSA (fPSA), percent fPSA, human glandular kallikrein 2 (hK2) and intact PSA measured three times within 2 weeks. Knowledge of the variation in an individual's PSA level is important for clinical decision-making. PATIENTS AND METHODS: Study participants were 149 patients referred for prostate biopsy, of which 97 had benign disease and 52 had prostate cancer. Three blood samples were drawn with a median of 4 h between first and second samples and 12 days between first and third samples. Variability was described by absolute differences, ratios and intra-individual coefficients of variation. Total PSA, fPSA, hK2 and intact PSA were measured in anticoagulated blood plasma. RESULTS: At baseline, the median tPSA was 6.8 (interquartile range, 4.5-9.6) ng/mL. The intra-individual variation was low for all biomarkers, and lowest for tPSA. For 80% of participants, the ratio between first and second time points for tPSA was in the range 0.91-1.09 and the ratio for percent fPSA was in the range 0.89-1.15. Total coefficients of variation between time 1 and 2 for tPSA, fPSA, percent fPSA, hK2 and intact PSA were 4.0%, 6.6%, 6.0%, 9.2% and 9.5%, respectively. The measurements taken several days apart varied more than those taken on the same day, although the variation between both time points was not large. CONCLUSIONS: The intra-individual variation for all the kallikrein-like markers studied was relatively small, especially for samples drawn the same day. Few cases are reclassified between the time points. This indicates the high short-term biological and technical reproducibility of the tests in clinical use. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1711022
- author
- Christensson, Anders
LU
; Bruun, Laila
LU
; Björk, Thomas
LU
; Cronin, Angel M
; Vickers, Andrew J
; Savage, Caroline J
and Lilja, Hans
LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BJU International
- volume
- 107
- pages
- 1769 - 1774
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000290770000010
- pmid:20955263
- scopus:79956369507
- pmid:20955263
- ISSN
- 1464-4096
- DOI
- 10.1111/j.1464-410X.2010.09761.x
- language
- English
- LU publication?
- yes
- id
- 79d2bf0d-0b98-484a-b74b-7712aef8de99 (old id 1711022)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20955263?dopt=Abstract
- date added to LUP
- 2016-04-04 07:49:15
- date last changed
- 2022-01-29 02:38:15
@article{79d2bf0d-0b98-484a-b74b-7712aef8de99,
abstract = {{Study Type - Diagnostic (exploratory cohort) Level of Evidence 2b OBJECTIVES: To assess variation of total prostate-specific antigen (tPSA), free PSA (fPSA), percent fPSA, human glandular kallikrein 2 (hK2) and intact PSA measured three times within 2 weeks. Knowledge of the variation in an individual's PSA level is important for clinical decision-making. PATIENTS AND METHODS: Study participants were 149 patients referred for prostate biopsy, of which 97 had benign disease and 52 had prostate cancer. Three blood samples were drawn with a median of 4 h between first and second samples and 12 days between first and third samples. Variability was described by absolute differences, ratios and intra-individual coefficients of variation. Total PSA, fPSA, hK2 and intact PSA were measured in anticoagulated blood plasma. RESULTS: At baseline, the median tPSA was 6.8 (interquartile range, 4.5-9.6) ng/mL. The intra-individual variation was low for all biomarkers, and lowest for tPSA. For 80% of participants, the ratio between first and second time points for tPSA was in the range 0.91-1.09 and the ratio for percent fPSA was in the range 0.89-1.15. Total coefficients of variation between time 1 and 2 for tPSA, fPSA, percent fPSA, hK2 and intact PSA were 4.0%, 6.6%, 6.0%, 9.2% and 9.5%, respectively. The measurements taken several days apart varied more than those taken on the same day, although the variation between both time points was not large. CONCLUSIONS: The intra-individual variation for all the kallikrein-like markers studied was relatively small, especially for samples drawn the same day. Few cases are reclassified between the time points. This indicates the high short-term biological and technical reproducibility of the tests in clinical use.}},
author = {{Christensson, Anders and Bruun, Laila and Björk, Thomas and Cronin, Angel M and Vickers, Andrew J and Savage, Caroline J and Lilja, Hans}},
issn = {{1464-4096}},
language = {{eng}},
pages = {{1769--1774}},
publisher = {{Wiley-Blackwell}},
series = {{BJU International}},
title = {{Intra-individual short-term variability of prostate-specific antigen and other kallikrein markers in a serial collection of blood from men under evaluation for prostate cancer.}},
url = {{http://dx.doi.org/10.1111/j.1464-410X.2010.09761.x}},
doi = {{10.1111/j.1464-410X.2010.09761.x}},
volume = {{107}},
year = {{2011}},
}