Cellulase production based on hemicellulose hydrolysate from steam-pretreated willow
(1997) In Applied Biochemistry and Biotechnology 63-5. p.351-362- Abstract
- The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO2 and steam-pretreated for 5 min at 206 degrees C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains... (More)
- The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO2 and steam-pretreated for 5 min at 206 degrees C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains degradation products such as acetic acid, furfural, and 5-hydroxy-methylfurfural, which are inhibitory for microorganisms. The results showed that 50% of the cellulose can be replaced with sugars from the wash water. The highest enzyme activity, 1.79 FPU/mL and yield, 133 FPU/g carbohydrate, was obtained at pH 6.0 using 20 g/L carbon source concentration. At lower pHs, a total lack of growth and enzyme production was observed, which probably could be explained by furfural inhibition. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911487
- author
- Szengyel, Z ; Zacchi, Guido LU and Reczey, K
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cellulase enzyme production, Trichoderma reesei Rut C 30, lignocellulosics, furfural inhibition
- in
- Applied Biochemistry and Biotechnology
- volume
- 63-5
- pages
- 351 - 362
- publisher
- Humana Press
- external identifiers
-
- wos:A1997XB48300032
- scopus:0001408419
- ISSN
- 1559-0291
- DOI
- 10.1007/BF02920437
- language
- English
- LU publication?
- yes
- id
- 7a5e44ee-fb37-4ea7-bb86-154985a9955e (old id 3911487)
- date added to LUP
- 2016-04-01 11:49:29
- date last changed
- 2023-09-01 08:42:23
@article{7a5e44ee-fb37-4ea7-bb86-154985a9955e,
abstract = {{The production cost of cellulolytic enzymes is a major contributor to the high cost of ethanol production from lignocellulosics using enzymatic hydrolysis. The aim of the present study was to investigate the cellulolytic enzyme production of Trichoderma reesei Rut C 30, which is known as a good cellulase secreting micro-organism, using willow as the carbon source. The willow, which is a fast-growing energy crop in Sweden, was impregnated with 1-4% SO2 and steam-pretreated for 5 min at 206 degrees C. The pretreated willow was washed and the wash water, which contains several soluble sugars from the hemicellulose, was supplemented with fibrous pretreated willow and used for enzyme production. In addition to sugars, the liquid contains degradation products such as acetic acid, furfural, and 5-hydroxy-methylfurfural, which are inhibitory for microorganisms. The results showed that 50% of the cellulose can be replaced with sugars from the wash water. The highest enzyme activity, 1.79 FPU/mL and yield, 133 FPU/g carbohydrate, was obtained at pH 6.0 using 20 g/L carbon source concentration. At lower pHs, a total lack of growth and enzyme production was observed, which probably could be explained by furfural inhibition.}},
author = {{Szengyel, Z and Zacchi, Guido and Reczey, K}},
issn = {{1559-0291}},
keywords = {{cellulase enzyme production; Trichoderma reesei Rut C 30; lignocellulosics; furfural inhibition}},
language = {{eng}},
pages = {{351--362}},
publisher = {{Humana Press}},
series = {{Applied Biochemistry and Biotechnology}},
title = {{Cellulase production based on hemicellulose hydrolysate from steam-pretreated willow}},
url = {{http://dx.doi.org/10.1007/BF02920437}},
doi = {{10.1007/BF02920437}},
volume = {{63-5}},
year = {{1997}},
}