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B cell profiling in malaria reveals expansion and remodeling of CD11c+ B cell subsets

Sundling, Christopher ; Rönnberg, Caroline ; Yman, Victor ; Asghar, Muhammad ; Jahnmatz, Peter ; Lakshmikanth, Tadepally ; Chen, Yang ; Mikes, Jaromir ; Forsell, Mattias N. and Sondén, Klara , et al. (2019) In JCI Insight 4(9).
Abstract

Humoral immunity is important in limiting clinical disease in malaria, yet the longitudinal B cell response to infection remains unclear. We performed a 1-year prospective study in patients treated for acute Plasmodium falciparum malaria for the first time or with previous exposure to the disease. Using an unbiased exploratory approach with mass cytometry, followed by targeted flow cytometry, we found that approximately 80% of mature B cells that proliferated in response to acute infection expressed CD11c. Only approximately 40% of CD11c+ B cells displayed an atypical B cell phenotype, with the remaining cells primarily made up of activated and resting memory B cells. The CD11c+ B cells expanded rapidly following... (More)

Humoral immunity is important in limiting clinical disease in malaria, yet the longitudinal B cell response to infection remains unclear. We performed a 1-year prospective study in patients treated for acute Plasmodium falciparum malaria for the first time or with previous exposure to the disease. Using an unbiased exploratory approach with mass cytometry, followed by targeted flow cytometry, we found that approximately 80% of mature B cells that proliferated in response to acute infection expressed CD11c. Only approximately 40% of CD11c+ B cells displayed an atypical B cell phenotype, with the remaining cells primarily made up of activated and resting memory B cells. The CD11c+ B cells expanded rapidly following infection, with previous exposure to malaria resulting in a significantly larger increase compared with individuals with primary infection. This was attributed to an expansion of switched CD11c+ B cells that was absent in primary infected individuals. The rate of contraction of the CD11c+ B cell compartment was independent of previous exposure to malaria and displayed a slow decay, with a half-life of approximately 300 days. Collectively, these results identify CD11c as a marker of B cells responding to malaria and further highlight differences in primary and secondary B cell responses during infection.

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JCI Insight
volume
4
issue
9
article number
e126492
publisher
The American Society for Clinical Investigation
external identifiers
  • scopus:85070659038
  • pmid:30939125
ISSN
2379-3708
DOI
10.1172/jci.insight.126492
language
English
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yes
id
7b10b7b3-0c55-4a58-aa5b-1dd4cec97b4a
date added to LUP
2019-08-27 11:57:24
date last changed
2020-04-05 06:11:41
@article{7b10b7b3-0c55-4a58-aa5b-1dd4cec97b4a,
  abstract     = {<p>Humoral immunity is important in limiting clinical disease in malaria, yet the longitudinal B cell response to infection remains unclear. We performed a 1-year prospective study in patients treated for acute Plasmodium falciparum malaria for the first time or with previous exposure to the disease. Using an unbiased exploratory approach with mass cytometry, followed by targeted flow cytometry, we found that approximately 80% of mature B cells that proliferated in response to acute infection expressed CD11c. Only approximately 40% of CD11c<sup>+</sup> B cells displayed an atypical B cell phenotype, with the remaining cells primarily made up of activated and resting memory B cells. The CD11c<sup>+</sup> B cells expanded rapidly following infection, with previous exposure to malaria resulting in a significantly larger increase compared with individuals with primary infection. This was attributed to an expansion of switched CD11c<sup>+</sup> B cells that was absent in primary infected individuals. The rate of contraction of the CD11c<sup>+</sup> B cell compartment was independent of previous exposure to malaria and displayed a slow decay, with a half-life of approximately 300 days. Collectively, these results identify CD11c as a marker of B cells responding to malaria and further highlight differences in primary and secondary B cell responses during infection.</p>},
  author       = {Sundling, Christopher and Rönnberg, Caroline and Yman, Victor and Asghar, Muhammad and Jahnmatz, Peter and Lakshmikanth, Tadepally and Chen, Yang and Mikes, Jaromir and Forsell, Mattias N. and Sondén, Klara and Achour, Adnane and Brodin, Petter and Persson, Kristina E.M. and Färnert, Anna},
  issn         = {2379-3708},
  language     = {eng},
  number       = {9},
  publisher    = {The American Society for Clinical Investigation},
  series       = {JCI Insight},
  title        = {B cell profiling in malaria reveals expansion and remodeling of CD11c<sup>+</sup> B cell subsets},
  url          = {http://dx.doi.org/10.1172/jci.insight.126492},
  doi          = {10.1172/jci.insight.126492},
  volume       = {4},
  year         = {2019},
}