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Drosophila stardust is a partner of Crumbs in the control of epithelial cell polarity

Bachmann, A.; Schneider, M. LU ; Theilenberg, E.; Grawe, F. and Knust, E. (2001) In Nature 414(6864). p.638-643
Abstract

The polarized architecture of epithelial cells depends on the highly stereotypic distribution of cellular junctions and other membrane-associated protein complexes. In epithelial cells of the Drosophila embryo, three distinct domains subdivide the lateral plasma membrane. The most apical one comprises the subapical complex (SAC). It is followed by the zonula adherens (ZA) and, further basally, by the septate junction1. A core component of the SAC is the transmembrane protein Crumbs, the cytoplasmic domain of which recruits the PDZ-protein Discs Lost into the complex2,3. Cells lacking crumbs or the functionally related gene stardust fail to organize a continuous ZA and to maintain cell polarity2,3. Here... (More)

The polarized architecture of epithelial cells depends on the highly stereotypic distribution of cellular junctions and other membrane-associated protein complexes. In epithelial cells of the Drosophila embryo, three distinct domains subdivide the lateral plasma membrane. The most apical one comprises the subapical complex (SAC). It is followed by the zonula adherens (ZA) and, further basally, by the septate junction1. A core component of the SAC is the transmembrane protein Crumbs, the cytoplasmic domain of which recruits the PDZ-protein Discs Lost into the complex2,3. Cells lacking crumbs or the functionally related gene stardust fail to organize a continuous ZA and to maintain cell polarity2,3. Here we show that stardust provides an essential component of the SAC. Stardust proteins colocalize with Crumbs and bind to the carboxy-terminal amino acids of its cytoplasmic tail. We introduce two different Stardust proteins here: one MAGUK protein, characterized by a PDZ domain, an SH3 domain and a guanylate kinase domain; and a second isoform comprising only the guanylate kinase domain. The Stardust proteins represent versatile candidates as structural and possibly regulatory constituents of the SAC, a crucial element in the control of epithelial cell polarity.

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author
publishing date
type
Contribution to journal
publication status
published
in
Nature
volume
414
issue
6864
pages
638 - 643
publisher
Nature Publishing Group
external identifiers
  • scopus:0035818984
ISSN
0028-0836
DOI
10.1038/414638a
language
English
LU publication?
no
id
7b4c7a99-c5ed-4522-95d7-d84ad8980595
date added to LUP
2017-01-12 11:16:00
date last changed
2018-08-12 04:32:42
@article{7b4c7a99-c5ed-4522-95d7-d84ad8980595,
  abstract     = {<p>The polarized architecture of epithelial cells depends on the highly stereotypic distribution of cellular junctions and other membrane-associated protein complexes. In epithelial cells of the Drosophila embryo, three distinct domains subdivide the lateral plasma membrane. The most apical one comprises the subapical complex (SAC). It is followed by the zonula adherens (ZA) and, further basally, by the septate junction<sup>1</sup>. A core component of the SAC is the transmembrane protein Crumbs, the cytoplasmic domain of which recruits the PDZ-protein Discs Lost into the complex<sup>2,3</sup>. Cells lacking crumbs or the functionally related gene stardust fail to organize a continuous ZA and to maintain cell polarity<sup>2,3</sup>. Here we show that stardust provides an essential component of the SAC. Stardust proteins colocalize with Crumbs and bind to the carboxy-terminal amino acids of its cytoplasmic tail. We introduce two different Stardust proteins here: one MAGUK protein, characterized by a PDZ domain, an SH3 domain and a guanylate kinase domain; and a second isoform comprising only the guanylate kinase domain. The Stardust proteins represent versatile candidates as structural and possibly regulatory constituents of the SAC, a crucial element in the control of epithelial cell polarity.</p>},
  author       = {Bachmann, A. and Schneider, M. and Theilenberg, E. and Grawe, F. and Knust, E.},
  issn         = {0028-0836},
  language     = {eng},
  month        = {12},
  number       = {6864},
  pages        = {638--643},
  publisher    = {Nature Publishing Group},
  series       = {Nature},
  title        = {Drosophila stardust is a partner of Crumbs in the control of epithelial cell polarity},
  url          = {http://dx.doi.org/10.1038/414638a},
  volume       = {414},
  year         = {2001},
}