Serum cystatin C measured by automated immunoassay : a more sensitive marker of changes in GFR than serum creatinine
Newman, D J ; Thakkar, H ; Edwards, R G ; Wilkie, M ; White, T ; Grubb, Anders O. LU
and Price, C P
(1995)
In Kidney International
47.
p.312-318
- Abstract
Serum cystatin C has been suggested as a new marker of GFR. For the introduction of this marker into clinical use a rapid and automated method is required. We have developed and validated an assay for serum cystatin C using latex particle-enhanced immunoturbidimetry. Intra- and inter-assay precision were < 3% and < 5% across the assay range. Analytical recovery was 93 +/- 3.8% and no lack of parallelism was demonstrated. Regression analysis of a method comparison with an enzyme-enhanced radial-immunodiffusion method, gave PETIA = 0.074 + 0.93 x SRID, r = 0.98, N = 100. Inter-assay precision profiles showed cystatin C was measured with two-fold better precision than creatinine on the same analyzer. Cystatin C measurement was... (More)
Serum cystatin C has been suggested as a new marker of GFR. For the introduction of this marker into clinical use a rapid and automated method is required. We have developed and validated an assay for serum cystatin C using latex particle-enhanced immunoturbidimetry. Intra- and inter-assay precision were < 3% and < 5% across the assay range. Analytical recovery was 93 +/- 3.8% and no lack of parallelism was demonstrated. Regression analysis of a method comparison with an enzyme-enhanced radial-immunodiffusion method, gave PETIA = 0.074 + 0.93 x SRID, r = 0.98, N = 100. Inter-assay precision profiles showed cystatin C was measured with two-fold better precision than creatinine on the same analyzer. Cystatin C measurement was neither interfered with by icterus nor by hemolysis. 1/cystatin C versus 1/creatinine concentrations gave r = 0.67, N = 469. Comparison of Cr EDTA GFR with 1/cystatin C and 1/creatinine gave r = 0.81 and 0.50, respectively, N = 206. Calculating diagnostic sensitivity for abnormal GFR showed cystatin C to be significantly (P < 0.05) more sensitive than creatinine (71.4 vs. 52.4%). Cystatin C measurement using PETIA technology can be automated on the same instruments used routinely for the measurement of creatinine and offers better analytical performance and probably improved clinical sensitivity as a screening test for early renal damage.
(Less)
- author
- Newman, D J
; Thakkar, H
; Edwards, R G
; Wilkie, M
; White, T
; Grubb, Anders O.
LU
and Price, C P
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biomarkers, Creatinine/blood, Cystatin C, Cystatins/blood, Cysteine Proteinase Inhibitors/blood, Glomerular Filtration Rate, Humans, Immunoassay/methods, Kidney Diseases/blood, Latex, Microspheres, Nephelometry and Turbidimetry/methods, Reproducibility of Results, Sensitivity and Specificity
- in
- Kidney International
- volume
- 47
- pages
- 312 - 318
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:7731163
- scopus:0028934503
- ISSN
- 0085-2538
- DOI
- 10.1038/ki.1995.40
- language
- English
- LU publication?
- yes
- id
- 7b53bb9d-19b1-4dc4-b98d-abe9eb78eae3
- date added to LUP
- 2021-10-28 14:40:23
- date last changed
- 2024-06-02 22:22:39
@article{7b53bb9d-19b1-4dc4-b98d-abe9eb78eae3,
abstract = {{<p>Serum cystatin C has been suggested as a new marker of GFR. For the introduction of this marker into clinical use a rapid and automated method is required. We have developed and validated an assay for serum cystatin C using latex particle-enhanced immunoturbidimetry. Intra- and inter-assay precision were < 3% and < 5% across the assay range. Analytical recovery was 93 +/- 3.8% and no lack of parallelism was demonstrated. Regression analysis of a method comparison with an enzyme-enhanced radial-immunodiffusion method, gave PETIA = 0.074 + 0.93 x SRID, r = 0.98, N = 100. Inter-assay precision profiles showed cystatin C was measured with two-fold better precision than creatinine on the same analyzer. Cystatin C measurement was neither interfered with by icterus nor by hemolysis. 1/cystatin C versus 1/creatinine concentrations gave r = 0.67, N = 469. Comparison of Cr EDTA GFR with 1/cystatin C and 1/creatinine gave r = 0.81 and 0.50, respectively, N = 206. Calculating diagnostic sensitivity for abnormal GFR showed cystatin C to be significantly (P < 0.05) more sensitive than creatinine (71.4 vs. 52.4%). Cystatin C measurement using PETIA technology can be automated on the same instruments used routinely for the measurement of creatinine and offers better analytical performance and probably improved clinical sensitivity as a screening test for early renal damage.</p>}},
author = {{Newman, D J and Thakkar, H and Edwards, R G and Wilkie, M and White, T and Grubb, Anders O. and Price, C P}},
issn = {{0085-2538}},
keywords = {{Biomarkers; Creatinine/blood; Cystatin C; Cystatins/blood; Cysteine Proteinase Inhibitors/blood; Glomerular Filtration Rate; Humans; Immunoassay/methods; Kidney Diseases/blood; Latex; Microspheres; Nephelometry and Turbidimetry/methods; Reproducibility of Results; Sensitivity and Specificity}},
language = {{eng}},
pages = {{312--318}},
publisher = {{Nature Publishing Group}},
series = {{Kidney International}},
title = {{Serum cystatin C measured by automated immunoassay : a more sensitive marker of changes in GFR than serum creatinine}},
url = {{http://dx.doi.org/10.1038/ki.1995.40}},
doi = {{10.1038/ki.1995.40}},
volume = {{47}},
year = {{1995}},
}