Polyethyleneimine-coated reticulated vitreous carbon electrode with immobilized enzymes as a substrate detector
(1998) In Analytica Chimica Acta 374(2-3). p.185-190- Abstract
Polyethyleneimine (PEI) was covalently coupled to carbodiimide-activated reticulated vitreous carbon (RVC). The PEI-coated RVC was activated with glutaraldehyde, and glycerol dehydrogenase and diaphorase were then immobilized. The PEI-coated RVC with immobilized enzymes functioned both as an enzyme reactor and a working electrode in an amperometric detection system where NAD+/NADH was recycled by the immobilized enzymes. The coated RVC electrode showed good properties compared to uncoated RVC, such as a long lifetime and a constant response to a series of injections in a flow-injection system, resulting in a relative standard deviation of 1.4%. The calibration graph was linear from the detection limit 0.1μM to 2mM NADH in the... (More)
Polyethyleneimine (PEI) was covalently coupled to carbodiimide-activated reticulated vitreous carbon (RVC). The PEI-coated RVC was activated with glutaraldehyde, and glycerol dehydrogenase and diaphorase were then immobilized. The PEI-coated RVC with immobilized enzymes functioned both as an enzyme reactor and a working electrode in an amperometric detection system where NAD+/NADH was recycled by the immobilized enzymes. The coated RVC electrode showed good properties compared to uncoated RVC, such as a long lifetime and a constant response to a series of injections in a flow-injection system, resulting in a relative standard deviation of 1.4%. The calibration graph was linear from the detection limit 0.1μM to 2mM NADH in the absence of recycling.
(Less)
- author
- Tang, Xiao-Jing LU ; Xie, Bin LU ; Larsson, Per-Olof LU ; Danielsson, Bengt LU ; Khayyami, Masoud and Johansson, Gillis LU
- organization
- publishing date
- 1998-11-23
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Diaphorase, Flow injection, Glycerol dehydrogenase, Polyethyleneimine, Reticulated vitreous carbon
- in
- Analytica Chimica Acta
- volume
- 374
- issue
- 2-3
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0031733892
- ISSN
- 0003-2670
- DOI
- 10.1016/S0003-2670(98)00255-4
- language
- English
- LU publication?
- yes
- id
- 7b81c96d-f7e2-4ec2-ad71-23a31dc39df3
- date added to LUP
- 2021-10-27 13:06:28
- date last changed
- 2025-10-14 10:37:38
@article{7b81c96d-f7e2-4ec2-ad71-23a31dc39df3,
abstract = {{<p>Polyethyleneimine (PEI) was covalently coupled to carbodiimide-activated reticulated vitreous carbon (RVC). The PEI-coated RVC was activated with glutaraldehyde, and glycerol dehydrogenase and diaphorase were then immobilized. The PEI-coated RVC with immobilized enzymes functioned both as an enzyme reactor and a working electrode in an amperometric detection system where NAD<sup>+</sup>/NADH was recycled by the immobilized enzymes. The coated RVC electrode showed good properties compared to uncoated RVC, such as a long lifetime and a constant response to a series of injections in a flow-injection system, resulting in a relative standard deviation of 1.4%. The calibration graph was linear from the detection limit 0.1μM to 2mM NADH in the absence of recycling.<br/></p>}},
author = {{Tang, Xiao-Jing and Xie, Bin and Larsson, Per-Olof and Danielsson, Bengt and Khayyami, Masoud and Johansson, Gillis}},
issn = {{0003-2670}},
keywords = {{Diaphorase; Flow injection; Glycerol dehydrogenase; Polyethyleneimine; Reticulated vitreous carbon}},
language = {{eng}},
month = {{11}},
number = {{2-3}},
pages = {{185--190}},
publisher = {{Elsevier}},
series = {{Analytica Chimica Acta}},
title = {{Polyethyleneimine-coated reticulated vitreous carbon electrode with immobilized enzymes as a substrate detector}},
url = {{http://dx.doi.org/10.1016/S0003-2670(98)00255-4}},
doi = {{10.1016/S0003-2670(98)00255-4}},
volume = {{374}},
year = {{1998}},
}