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Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis

Li, Kan Yan Chloe ; Dejea, Hector LU ; De Winne, Koen ; Bonnin, Anne ; D’Onofrio, Valentino ; Cox, Janneke A. ; Garcia-Canadilla, Patricia ; Lammens, Martin ; Cook, Andrew C. and Bijnens, Bart , et al. (2023) In Histochemistry and Cell Biology 160(5). p.377-389
Abstract

X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Needle biopsies, Pathology, X-ray phase contrast imaging
in
Histochemistry and Cell Biology
volume
160
issue
5
pages
13 pages
publisher
Springer
external identifiers
  • scopus:85166263026
  • pmid:37523091
ISSN
0948-6143
DOI
10.1007/s00418-023-02220-6
language
English
LU publication?
yes
id
7b8acf9c-185d-4496-86ae-485ea2724a33
date added to LUP
2023-11-21 14:45:12
date last changed
2024-06-13 23:09:59
@article{7b8acf9c-185d-4496-86ae-485ea2724a33,
  abstract     = {{<p>X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.</p>}},
  author       = {{Li, Kan Yan Chloe and Dejea, Hector and De Winne, Koen and Bonnin, Anne and D’Onofrio, Valentino and Cox, Janneke A. and Garcia-Canadilla, Patricia and Lammens, Martin and Cook, Andrew C. and Bijnens, Bart and Dendooven, Amélie}},
  issn         = {{0948-6143}},
  keywords     = {{Needle biopsies; Pathology; X-ray phase contrast imaging}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{377--389}},
  publisher    = {{Springer}},
  series       = {{Histochemistry and Cell Biology}},
  title        = {{Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis}},
  url          = {{http://dx.doi.org/10.1007/s00418-023-02220-6}},
  doi          = {{10.1007/s00418-023-02220-6}},
  volume       = {{160}},
  year         = {{2023}},
}