Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis
(2023) In Histochemistry and Cell Biology 160(5). p.377-389- Abstract
X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.
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https://lup.lub.lu.se/record/7b8acf9c-185d-4496-86ae-485ea2724a33
- author
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Needle biopsies, Pathology, X-ray phase contrast imaging
- in
- Histochemistry and Cell Biology
- volume
- 160
- issue
- 5
- pages
- 13 pages
- publisher
- Springer
- external identifiers
-
- scopus:85166263026
- pmid:37523091
- ISSN
- 0948-6143
- DOI
- 10.1007/s00418-023-02220-6
- language
- English
- LU publication?
- yes
- id
- 7b8acf9c-185d-4496-86ae-485ea2724a33
- date added to LUP
- 2023-11-21 14:45:12
- date last changed
- 2024-06-13 23:09:59
@article{7b8acf9c-185d-4496-86ae-485ea2724a33, abstract = {{<p>X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.</p>}}, author = {{Li, Kan Yan Chloe and Dejea, Hector and De Winne, Koen and Bonnin, Anne and D’Onofrio, Valentino and Cox, Janneke A. and Garcia-Canadilla, Patricia and Lammens, Martin and Cook, Andrew C. and Bijnens, Bart and Dendooven, Amélie}}, issn = {{0948-6143}}, keywords = {{Needle biopsies; Pathology; X-ray phase contrast imaging}}, language = {{eng}}, number = {{5}}, pages = {{377--389}}, publisher = {{Springer}}, series = {{Histochemistry and Cell Biology}}, title = {{Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis}}, url = {{http://dx.doi.org/10.1007/s00418-023-02220-6}}, doi = {{10.1007/s00418-023-02220-6}}, volume = {{160}}, year = {{2023}}, }