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Escherichia coli ccm in-frame mutants can produce periplasmic cytochrome b but not cytochrome c

Throne-Holst, Mimmi LU ; Thöny-Meyer, Linda and Hederstedt, Lars LU (1997) In FEBS Letters 410(2-3).
Abstract
Escherichia coli CcmA, CcmB and CcmC polypeptides are required for cytochrome c synthesis and are thought to constitute the subunits of an ABC-type transporter as judged from sequence data, Using a periplasmic reporter system based on Bacillus subtilis cytochrome c-550 and E. coli cytochrome b-562 we show that the synthesis of the b-type cytochrome in the periplasm is normal in E, coli ccmA and ccmC in-frame deletion mutants, Mutants deleted for ccmF or ccmG encoding a component of a putative cytochrome c-heme lyase and a membrane bound thioredoxin-like protein, respectively, have the same phenotype, The ccm mutants produce cytochrome c-550 polypeptide, but not holocytochrome c, Taken together the results demonstrate that heme can be... (More)
Escherichia coli CcmA, CcmB and CcmC polypeptides are required for cytochrome c synthesis and are thought to constitute the subunits of an ABC-type transporter as judged from sequence data, Using a periplasmic reporter system based on Bacillus subtilis cytochrome c-550 and E. coli cytochrome b-562 we show that the synthesis of the b-type cytochrome in the periplasm is normal in E, coli ccmA and ccmC in-frame deletion mutants, Mutants deleted for ccmF or ccmG encoding a component of a putative cytochrome c-heme lyase and a membrane bound thioredoxin-like protein, respectively, have the same phenotype, The ccm mutants produce cytochrome c-550 polypeptide, but not holocytochrome c, Taken together the results demonstrate that heme can be transported to the periplasm by a ccm-independent mechanism. (C) 1997 Federation of European Biochemical Societies. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
410
issue
2-3
publisher
Wiley-Blackwell
external identifiers
  • scopus:17044454865
ISSN
1873-3468
DOI
10.1016/S0014-5793(97)00656-X
language
English
LU publication?
yes
id
7b905fe6-cfc7-48a0-99ee-8a7bfcb54b74
date added to LUP
2017-07-18 09:59:43
date last changed
2017-11-12 04:33:47
@article{7b905fe6-cfc7-48a0-99ee-8a7bfcb54b74,
  abstract     = {Escherichia coli CcmA, CcmB and CcmC polypeptides are required for cytochrome c synthesis and are thought to constitute the subunits of an ABC-type transporter as judged from sequence data, Using a periplasmic reporter system based on Bacillus subtilis cytochrome c-550 and E. coli cytochrome b-562 we show that the synthesis of the b-type cytochrome in the periplasm is normal in E, coli ccmA and ccmC in-frame deletion mutants, Mutants deleted for ccmF or ccmG encoding a component of a putative cytochrome c-heme lyase and a membrane bound thioredoxin-like protein, respectively, have the same phenotype, The ccm mutants produce cytochrome c-550 polypeptide, but not holocytochrome c, Taken together the results demonstrate that heme can be transported to the periplasm by a ccm-independent mechanism. (C) 1997 Federation of European Biochemical Societies.},
  author       = {Throne-Holst, Mimmi and Thöny-Meyer, Linda and Hederstedt, Lars},
  issn         = {1873-3468},
  language     = {eng},
  number       = {2-3},
  publisher    = {Wiley-Blackwell},
  series       = {FEBS Letters},
  title        = {<em>Escherichia coli ccm</em> in-frame mutants can produce periplasmic cytochrome b but not cytochrome c},
  url          = {http://dx.doi.org/10.1016/S0014-5793(97)00656-X},
  volume       = {410},
  year         = {1997},
}