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A direct screen for secreted proteins in Xenopus embryos identifies distinct activities for the Wnt antagonists Crescent and Frzb-1

Pera, Edgar LU and De Robertis, E M (2000) In Mechanisms of Development 96(2). p.183-195
Abstract
To determine the spectrum of secreted proteins that are present in the extracellular space of early Xenopus embryos, a direct secretion screen was performed. Surprisingly, 24% of previously identified bona fide secretory proteins corresponded to four secreted Wnt antagonists of the same family: frzb-1, sizzled, sfrp-2 and crescent. sfrp-2 and crescent are novel components of the growing cocktail of growth factor antagonists secreted by Spemann organizer cells in Xenopus. Crescent is first expressed at blastula, defining a deep endodermal region that may be homologous to the avian hypoblast. Unlike other members of this family of inhibitors, microinjection of crescent mRNA causes the development of cyclopic embryos, even though the amount... (More)
To determine the spectrum of secreted proteins that are present in the extracellular space of early Xenopus embryos, a direct secretion screen was performed. Surprisingly, 24% of previously identified bona fide secretory proteins corresponded to four secreted Wnt antagonists of the same family: frzb-1, sizzled, sfrp-2 and crescent. sfrp-2 and crescent are novel components of the growing cocktail of growth factor antagonists secreted by Spemann organizer cells in Xenopus. Crescent is first expressed at blastula, defining a deep endodermal region that may be homologous to the avian hypoblast. Unlike other members of this family of inhibitors, microinjection of crescent mRNA causes the development of cyclopic embryos, even though the amount of anterior neural tissue is normal. In crescent-injected embryos, studies with specific markers indicate that morphogenetic movements of the anterior midline are abnormal, resulting in a more posterior location of prechordal plate and ventral forebrain markers with respect to the developing eye field. The results are discussed in light of recent findings in zebrafish and Xenopus that suggest that Wnt signaling through non-canonical (non-beta-catenin dependent) pathways plays a pivotal role in the regulation of morphogenetic movements. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
in
Mechanisms of Development
volume
96
issue
2
pages
183 - 195
publisher
Elsevier
external identifiers
  • scopus:0034705993
ISSN
0925-4773
DOI
10.1016/S0925-4773(00)00394-4
language
English
LU publication?
no
id
7bbd5f1e-cacb-427a-acf3-6ae7891b84cf
date added to LUP
2018-02-20 14:57:12
date last changed
2018-12-16 05:06:05
@article{7bbd5f1e-cacb-427a-acf3-6ae7891b84cf,
  abstract     = {To determine the spectrum of secreted proteins that are present in the extracellular space of early Xenopus embryos, a direct secretion screen was performed. Surprisingly, 24% of previously identified bona fide secretory proteins corresponded to four secreted Wnt antagonists of the same family: frzb-1, sizzled, sfrp-2 and crescent. sfrp-2 and crescent are novel components of the growing cocktail of growth factor antagonists secreted by Spemann organizer cells in Xenopus. Crescent is first expressed at blastula, defining a deep endodermal region that may be homologous to the avian hypoblast. Unlike other members of this family of inhibitors, microinjection of crescent mRNA causes the development of cyclopic embryos, even though the amount of anterior neural tissue is normal. In crescent-injected embryos, studies with specific markers indicate that morphogenetic movements of the anterior midline are abnormal, resulting in a more posterior location of prechordal plate and ventral forebrain markers with respect to the developing eye field. The results are discussed in light of recent findings in zebrafish and Xenopus that suggest that Wnt signaling through non-canonical (non-beta-catenin dependent) pathways plays a pivotal role in the regulation of morphogenetic movements.},
  author       = {Pera, Edgar and De Robertis, E M},
  issn         = {0925-4773},
  language     = {eng},
  number       = {2},
  pages        = {183--195},
  publisher    = {Elsevier},
  series       = {Mechanisms of Development},
  title        = {A direct screen for secreted proteins in Xenopus embryos identifies distinct activities for the Wnt antagonists Crescent and Frzb-1},
  url          = {http://dx.doi.org/10.1016/S0925-4773(00)00394-4},
  volume       = {96},
  year         = {2000},
}